Wheat heat tolerance is impaired by heightened deletions in the distal end of 4AL chromosomal arm

Zhai, Huijie; Jiang, Congcong; Zhao, Yue; Yang, Suzhen; Li, Yiwen; Yan, Kunfang; Wu, Shu-Yu; Luo, Bingke; Du, Yi; Jin, Huaibing; Liu, Xin; Zhang, Yanbing; Lü, Fei; Reynolds, Matthew; Ou, Xingqi; Qiao, Wenchen; Jiang, Zhikai; Peng, Tao; Gao, Derong; Hu, Wenjing; Jiang-chun, Wang; Gao, Haitao; Guihong, Yin; Zhang, Kunpu; Li, Guangwei; Wang, Daowen

doi:10.1111/pbi.13529

Cited by 16 publications

(16 citation statements)

References 87 publications

(106 reference statements)

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“…The variation at ELF3 might have occurred because of the positioning of ELF3 within the subtelomeric region (Aguilar and Prieto 2020) which has enhanced levels of recombination which can lead to increased number of sequence and structural variants. This has been observed for other regions, recent studies have reported that a deletion at the distal end of chromosome 4AL is associated with altered heat tolerance (Zhai et al . 2021) and a deletion at the distal end of 5AL encompasses the Rht12 locus which is associated with dwarfing (Sun et al .…”

Section: Discussionsupporting

confidence: 69%

“…The variation at ELF3 might have occurred because of the positioning of ELF3 within the subtelomeric region (56) which has enhanced levels of recombination which can lead to increased number of sequence and structural variants. This has been observed for other regions, recent studies have reported that a deletion at the distal end of chromosome 4AL is associated with altered heat tolerance (57) and a deletion at the distal end of 5AL encompasses the Rht12 locus which is associated with dwarfing (58). Secondly, variation at ELF3 may have been selected by past breeding efforts, leading to a change in allele frequencies, due to advantageous adaptation of flowering time to local climates similar to that seen for selection of Ppd-1 alleles based on latitudinal cline (59) and altitude (60).…”

Section: Wheat Elf3 Might Be a Promising Breeding Targetsupporting

confidence: 67%

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WheatEARLY FLOWERING3is a dawn-expressed circadian oscillator component that regulates heading date

Wittern

Steed

Taylor

et al. 2021

Preprint

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Optimising the seasonal control of flowering in the major crops is an important component of breeding to match crop adaptation to the target environment. Using an eight parent Multiparent Advanced Generation Inter-Cross (MAGIC) population we investigated the contribution of variation at circadian clock-associated genes to the regulation of heading date (flowering) in UK and European winter wheat varieties. We identified homoeologues of EARLY FLOWERING 3 (ELF3) as candidate genes for the Earliness per se (Eps) D1 and B1 loci in field conditions. We confirmed that a SNP within the coding region of TaELF3-B1 is the likely causal polymorphism underlying the Eps-B1 locus. We also identified that a reported deletion at the Eps-D1 locus encompassing TaELF3-D1, is in fact a novel allele that lies within an introgression region that contains an inversion relative to the Chinese Spring D genome. Our findings that ELF3 might be associated with the regulation of heading date prompted us to investigate whether ELF3 is a circadian oscillator gene in wheat, as it is in Arabidopsis. Using T. turgidum cv. Kronos carrying loss of function alleles for both copies of TtELF3 we found that circadian rhythms were severely disrupted. Furthermore, in T. aestivum, we found that loss of functional LUX ARRHYTHMO (LUX), an orthologue of the protein partner of ELF3 in Arabidopsis, also severely disrupted circadian rhythms. Whilst these data suggest a function for both ELF3 and LUX in the wheat circadian oscillator, that oscillator might be structured differently to that of Arabidopsis because wheat ELF3 and LUX transcripts are maximal at the end of the night and day respectively, rather than co-expressed at dusk as they are in Arabidopsis. We conclude that there is sufficient allelic diversity within the three wheat ELF3 homoeologues for selection to delay or advance heading, and that this can be achieved without pleiotropic deleterious alterations to circadian rhythms.

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Section: Discussionsupporting

confidence: 69%

Section: Wheat Elf3 Might Be a Promising Breeding Targetsupporting

confidence: 67%

WheatEARLY FLOWERING3is a dawn-expressed circadian oscillator component that regulates heading date

Wittern

Steed

Taylor

et al. 2021

Preprint

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“…It is very challenging to isolate the genes responsible for HST in wheat by map-based cloning although heat tolerant mutants were identified 20 years ago ( Mullarkey and Jones, 2000 ). TaHST1 is a QTL that is significantly associated with HST in both vegetative and reproductive stages of wheat ( Zhai et al, 2021 ). It was mapped on the distal terminal of chr4A.…”

Section: Introductionmentioning

confidence: 99%

“…According to reference genome sequence of Chinese spring (CS), this 0.949 Mb region has 19 high confidence genes ( IWGSC, 2018 ). Five markers Xhau1-Xhau5 were used for the detection different haplotypes in this region in wheat cultivars ( Zhai et al, 2021 ). Further investigation revealed that an unusual high number of deletion mutation in this region was observed, which was confirmed by using sequencing data from 10+ wheat genome project.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars

et al. 2022

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Increasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance.

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“…Therefore, emphasis should be placed on sustaining the wheat yields through the identification of tolerant genotypes and promotion of breeding strategies and management practices that can help to build HS resilience and safeguard the wheat production from HS [ 22 ]. An improved understanding of morphological and physiological traits associated with HS tolerance has pragmatic implications for devising countermeasures, e.g., to identify various tolerance mechanisms and their use in alleviation strategies [ 23 , 24 , 25 ]. The mapping of genomic regions governing such physiological traits helped to identify genes (or QTLs) conferring HS tolerance, which serve as a strong base for the marker-assisted (MAS) breeding of HS tolerance in wheat [ 24 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Impacts, Tolerance, Adaptation, and Mitigation of Heat Stress on Wheat under Changing Climates

Yadav

Choudhary

Singh

et al. 2022

IJMS

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Heat stress (HS) is one of the major abiotic stresses affecting the production and quality of wheat. Rising temperatures are particularly threatening to wheat production. A detailed overview of morpho-physio-biochemical responses of wheat to HS is critical to identify various tolerance mechanisms and their use in identifying strategies to safeguard wheat production under changing climates. The development of thermotolerant wheat cultivars using conventional or molecular breeding and transgenic approaches is promising. Over the last decade, different omics approaches have revolutionized the way plant breeders and biotechnologists investigate underlying stress tolerance mechanisms and cellular homeostasis. Therefore, developing genomics, transcriptomics, proteomics, and metabolomics data sets and a deeper understanding of HS tolerance mechanisms of different wheat cultivars are needed. The most reliable method to improve plant resilience to HS must include agronomic management strategies, such as the adoption of climate-smart cultivation practices and use of osmoprotectants and cultured soil microbes. However, looking at the complex nature of HS, the adoption of a holistic approach integrating outcomes of breeding, physiological, agronomical, and biotechnological options is required. Our review aims to provide insights concerning morpho-physiological and molecular impacts, tolerance mechanisms, and adaptation strategies of HS in wheat. This review will help scientific communities in the identification, development, and promotion of thermotolerant wheat cultivars and management strategies to minimize negative impacts of HS.

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Wheat heat tolerance is impaired by heightened deletions in the distal end of 4AL chromosomal arm

Cited by 16 publications

References 87 publications

WheatEARLY FLOWERING3is a dawn-expressed circadian oscillator component that regulates heading date

WheatEARLY FLOWERING3is a dawn-expressed circadian oscillator component that regulates heading date

Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars

Impacts, Tolerance, Adaptation, and Mitigation of Heat Stress on Wheat under Changing Climates

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