Wheat-germ agglutinin binding in four types of mouse peritoneal macrophage

Abstract

After stimulation of the mouse peritoneal cavity with newborn calf serum (NBCS), four types of monocyte and macrophage were distinguished on the basis of peroxidase (PO) patterns. These cell types showed heterogeneity in their binding of the lectin wheat-germ agglutinin (WGA). At 16 h after stimulation, monocytes and monocyte-derived macrophages (with PO activity in granules) had a high level of WGA binding; PO-negative macrophages showed moderate WGA binding, and resident macrophages (with PO activity in the RER and nuclear envelope) had low WGA binding. At later time-points after stimulation, each of these cell types lost WGA binding sites. This decrease was related to a process of differentiation and to a modulation, affected by environmental factors. The present results also indicated that PO-negative macrophages can give rise to resident macrophages. Whether these PO-negative cells are monocyte derived or originate otherwise needs further investigation. The fourth type of macrophage, the exudate-resident cell (with PO activity both in granules and in the RER and nuclear envelope), with a WGA binding pattern similar to that of monocytes and monocyte-derived macrophages, was considered not to be a resident precursor cell.

Expression of 5′Nucleotidase Activity and Wheat-Germ Agglutinin Binding Sites in Mononuclear Phagocytes From Bone Marrow Cultures

“…These cells subsequently acquired PO activity in the RER and NE. The assumption that PO-negative macrophages are precursor cells of resident macrophages is in line with previous data, from which it could be deduced that the PO-negative macrophages are related to resident macrophages [9][10][11][12]17). An interesting question, then, is whether these PO-negative macrophages are monocytederived.…”

“…Thus, the expression of WGA binding sites seems to diminish during the differentia tion of promonocytes to exudate macrophages. This assumption is supported by results of a previous study, which showed that blood monocytes express more WGA binding sites than monocytes and macrophages from the peritoneal cavity and that peritoneal exudate macrophages lose WGA binding sites with increasing time after intraperitoneal injection of a stimulus [12]. Under the latter experimental conditions,…”

“…Another population that might represent transitional forms between monocytes and resident macrophages concerns the PO-negative macrophages. The nature of the latter cell type was previously not clear [12,13,18]. In the present study the POnegative macrophages occurred in large numbers at a time when the numbers of resident macrophages had started to increase significantly.…”

“…In previous studies [2,[11][12][13] on monocytes and macrophages from the perito neal cavity, use was made of two cytochemical techniques, each combined with the demonstration of PO activity. One of these, a gold labeling procedure, offers a quantitative method for the assessment of binding sites for the lectin wheat-germ agglutinin (WGA): the other concerns the ectoenzyme 5 'nucleotidase (5'N).…”

Invasion and survival ofFusarium solaniin the dexamethasone-treated cornea of rabbits

“…The specimens were incubated in 0-1 M cacodylate buffer (pH 7.4) with 0-5 mg m1-1 3,3'-diaminobenzidine (DAB, Sigma) and 0.01% H202 for 4 h at 37°C to demonstrate peroxidase activity of the neutrophils [4]. For examination of catalase activity, the samples were incubated in 2 mg ml -~ DAB and 0.03% H202 in 0.05 M propandiol buffer (pH 9.5) for 4 h at 37°C.…”