Wheat Germ Agglutinin-Binding Glycoprotein Extract from Shells of Conspecifics Induces Settlement of Larvae of the Pacific Oyster<i>Crassostrea gigas</i>(Thunberg)

Vásquez, Hebert Ely; Hashimoto, Kyotaro; Kitamura, Hitoshi; Satuito, Cyril Glenn

doi:10.2983/035.033.0210

Cited by 10 publications

(26 citation statements)

References 34 publications

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“…The adsorption of CgSE on GF/C, Polystyrene, and PVDF substrates resulted in similar larval settlement responses, which indicate that any of these substrates are suitable for use. Relatively similar results were also reported by Vazquez et al (2014) , where the highest number of post-larvae were recorded in GF/C and glass substrates [ 9 ]. Therefore, the direct application of the CgSE on polystyrene wells was used in the sugar screening and time exposure assays, while the GF/C filter papers were used in the lectin-sugar interaction assays.…”

Section: Discussionsupporting

confidence: 89%

“…Although the active search for suitable substrate or settlement sites, in response to a wide range of variables and cues, has been well-documented in oyster larvae [ 1 , 2 , 8 , 9 , 11 , 16 , 23 , 32 ], the exact mechanism(s) of how larval settlements are induced is not yet fully understood. Understanding these processes and cues can help us understand the underlying mechanisms that shape population structures and chemical signaling.…”

Section: Discussionmentioning

confidence: 99%

“…A wide array of waterborne or surface-bound cues associated with conspecific adults have been reported to induce larval settlement. These include shells in C. gigas [ 8 , 9 ], C. virginica [ 10 , 11 ], C. ariakensis [ 12 ]; soft tissue homogenates in Ostrea edulis [ 13 ]; water pre-conditioned by adults of C. ariakensis [ 12 ], C. gigas [ 14 ], C. virginica [ 15 , 16 , 17 ], and O. puelchana [ 18 ]; oyster shell liquor or extrapallial fluid of C. virginica [ 19 ]; and ammonia [ 6 ]. Another source of such cues are chemicals bound to or released from bacterial biofilms in the form of extracellular polymeric substances [ 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Earlier works in other marine invertebrates, such as barnacles have shown that the presence of dissolved sugars influences the exploration, identification of conspecific cues, and larval metamorphosis [ 29 , 30 , 31 ]. Also, Vasquez et al (2014) have provided evidence that a WGA-binding sugar chain in shells of conspecifics may mediate the settlement of C. gigas larvae on conspecifics, thus, highlighting the role played by lectin-glycan in biorecognition [ 8 , 9 ]. However, the nature of the chemical cue from shells of C. gigas is not yet fully characterized and elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas

Sedanza

Kim

Seposo

et al. 2021

IJMS

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This study evaluated the larval settlement inducing effect of sugars and a conspecific cue from adult shell extract of Crassostrea gigas. To understand how the presence of different chemical cues regulate settlement behavior, oyster larvae were exposed to 12 types of sugars, shell extract-coated and non-coated surfaces, and under varied sugar exposure times. Lectin-glycan interaction effects on settlement and its localization on oyster larval tissues were investigated. The results showed that the conspecific cue elicited a positive concentration dependent settlement inducing trend. Sugars in the absence of a conspecific cue, C. gigas adult shell extract, did not promote settlement. Whereas, in the presence of the cue, showed varied effects, most of which were found inhibitory at different concentrations. Sugar treated larvae exposed for 2 h showed significant settlement inhibition in the presence of a conspecific cue. Neu5Ac, as well as GlcNAc sugars, showed a similar interaction trend with wheat germ agglutinin (WGA) lectin. WGA-FITC conjugate showed positive binding on the foot, velum, and mantle when exposed to GlcNAc sugars. This study suggests that a WGA lectin-like receptor and its endogenous ligand are both found in the larval chemoreceptors and the shell Ethylenediaminetetraacetic acid (EDTA) extract that may complementarily work together to allow the oyster larva greater selectivity during site selection.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas

Sedanza

Kim

Seposo

et al. 2021

IJMS

Self Cite

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“…Such species‐specific adaptations to micro‐environmental conditions have been reported for other species and are thought to be crucial for survival and growth. For example, oyster larvae settle and metamorphose preferentially on conspecific adult shells, which contain glycoprotein‐inducing substances within the organic shell matrix (Vasquez, Hashimoto, Yoshida, Hara, Imai, Kitamura, & Satuito ; Vasquez, Hashimoto, Kitamura, & Satuito ). Similarly, abalone larvae may settle as a response to mucus trails of conspecific juveniles, which may signal suitable settlement substrates (Gallardo & Buen ; Laimek, Clark, Stewart, Pfeffer, Wanichanon, Hanna, & Sobhon ).…”

Section: Discussionmentioning

confidence: 99%

Effect of neuroactive compounds on larval metamorphosis of New Zealand geoduck (Panopea zelandica )

Young

Watts

et al. 2016

Aquac Res

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Please cite this article as: Young, Tim, Alfaro, Andrea C., Robertson, John, Effect of neuroactive compounds on the settlement of mussel (Perna canaliculus) larvae, Aquaculture (2011), doi: 10.1016/j.aquaculture.2011.06.050 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractHerein, we present the first laboratory study on the effects of pharmacologically active compounds on larval settlement of the green-lipped mussel, Perna canaliculus.Competent hatchery-reared larvae were exposed to seawater containing excess K + in the form of KCl and K 2 SO 4 and the neurotransmitters γ-aminobutyric acid (GABA) and acetylcholine.Both KCl and K 2 SO 4 were identified as active inducers of larval settlement with maximum inductions occurring after exposures to 10 and 7.5 mM, respectively. Peak settlement response to KCl was higher (>64%) than that achieved with K 2 SO 4 (>41%). GABA did not induce larval settlement and displayed toxic and settlement inhibitive effects at 10 -4 and 10 -3M. Acetylcholine induced larval settlement (>49%) at 10 -4 M with minimal acute toxic effects (LC < 10%). To gain insight into the class of acetylcholine receptors involved, atropine was used to block the muscarinic-type receptors. Atropine treatment alone did not inhibit settlement compared to control assays, indicating that muscarinic-type receptors are not involved in settlement behavior. Furthermore, results showed that atropine did not significantly decrease acetylcholine induced settlement responses, which suggests an active role of the nicotinic-type receptors in the biochemical pathways of mussel settlement.Results of this study provide new insights on the mechanism of settlement behavior in P.canaliculus, which may have direct application to the growing New Zealand aquaculture industry.

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Conservation and restoration of a keystone species: Understanding the settlement preferences of the European oyster (Ostrea edulis)

Rodríguez-Pérez

James

Donnan

et al. 2019

Marine Pollution Bulletin

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Wheat Germ Agglutinin-Binding Glycoprotein Extract from Shells of Conspecifics Induces Settlement of Larvae of the Pacific OysterCrassostrea gigas(Thunberg)

Cited by 10 publications

References 34 publications

Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas

Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas

Effect of neuroactive compounds on larval metamorphosis of New Zealand geoduck (Panopea zelandica )

Conservation and restoration of a keystone species: Understanding the settlement preferences of the European oyster (Ostrea edulis)

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