Wheat Flour Enzymatic Amylolysis Monitored by in Situ<sup>1</sup>H NMR Spectroscopy

Amato, Maria E.; Ansanelli, Giuliana; Fisichella, Salvatore; Lamanna, Raffaele; Scarlata, Giuseppe; Sobolev, Anatoly P.; Segre, Annalaura

doi:10.1021/jf035188v

Cited by 30 publications

(26 citation statements)

References 12 publications

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“…When high-resolution NMR methods are coupled to multivariate statistical analysis, the resulting partitions give unambiguous information about influences of nutritional and genetic backgrounds (Ratcliffe and Shachar-Hill, 2001;Mannina et al, 2003;Amato et al, 2004;Krishnan et al, 2005). Metabolite profiling, metabolomics, offers a powerful approach to monitor complexity of genetically modified crops and document any unintended consequences of a modified gene introduced in crops (Kuiper et al, 2001;Sumner et al, 2003;Tretheway, 2004;Mungur et al, 2005).…”

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confidence: 99%

Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions

et al. 2006

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Polyamines are ubiquitous aliphatic amines that have been implicated in myriad processes, but their precise biochemical roles are not fully understood. We have carried out metabolite profiling analyses of transgenic tomato (Solanum lycopersicum) fruit engineered to accumulate the higher polyamines spermidine (Spd) and spermine (Spm) to bring an insight into the metabolic processes that Spd/Spm regulate in plants. NMR spectroscopic analysis revealed distinct metabolite trends in the transgenic and wild-type/azygous fruits ripened off the vine. Distinct metabolites (glutamine, asparagine, choline, citrate, fumarate, malate, and an unidentified compound A) accumulated in the red transgenic fruit, while the levels of valine, aspartic acid, sucrose, and glucose were significantly lower as compared to the control (wild-type and azygous) red fruit. The levels of isoleucine, glucose, g-aminobutyrate, phenylalanine, and fructose remained similar in the nontransgenic and transgenic fruits. Statistical treatment of the metabolite variables distinguished the control fruits from the transgenic fruit and provided credence to the pronounced, differential metabolite profiles seen during ripening of the transgenic fruits. The pathways involved in the nitrogen sensing/signaling and carbon metabolism seem preferentially activated in the high Spd/Spm transgenics. The metabolite profiling analysis suggests that Spd and Spm are perceived as nitrogenous metabolites by the fruit cells, which in turn results in the stimulation of carbon sequestration. This is seen manifested in higher respiratory activity and up-regulation of phosphoenolpyruvate carboxylase and NADP-dependent isocitrate dehydrogenase transcripts in the transgenic fruit compared to controls, indicating high metabolic status of the transgenics even late in fruit ripening.

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confidence: 99%

Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions

et al. 2006

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“…In particular, for the three selected thresholds, namely 0.32, 0.27, and 0.19%, the number of buckets obtained were 565, 607, and 716, respectively. Assignment of NMR signals to the corresponding chemical compound was made by using the literature data [7,19,23] and confirmed by previous made 2D NMR experiments. [7] Statistical analysis From the bucketing procedure, the whole spectrum was reduced to more than 500 variables, which were still too many compared to the number of samples.…”

Section: Nuclear Magnetic Resonancementioning

confidence: 83%

“…[7] For each sample, 120 mg of whole meal was dissolved in 1.3 ml of buffer. After 60 min, the suspension was centrifuged at 14 000 r.p.m.…”

Section: Samples Preparationmentioning

confidence: 99%

“…Unfortunately, the HR-MAS spectra were dominated, in the high-field region, by the broad signals of lipids which actually did not correlate with the samples origin and masked a significant number of low intensity resonances related to amino and organic acids. [7] For these reasons, in this paper we analyzed, by 1 H-NMR, several aqueous extracts of wheat flours of different cultivars and from different places of Apulia region (Southern Italy) in order to try to establish a clear correlation between 1 H-NMR spectra and geographical or botanical origin of wheat samples.…”

Section: Introductionmentioning

confidence: 99%

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Geographical origin of durum wheat studied by ¹H‐NMR profiling

Lamanna

Cattivelli

Miglietta

et al. 2010

Magnetic Reson in Chemistry

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(1)H-Nuclear magnetic resonance (NMR) spectroscopy was used to detect metabolic profiles of wheat flour samples of different geographical and botanical origin. The NMR profiles were analyzed by multivariate statistical techniques in order to establish the origin of the samples. A linear model, able to discriminate among three different locations, was built achieving a prediction level of about 80% of correctly assigned samples. The principal classes of compounds responsible for the geographic origin discrimination were individuated in aromatic compounds and amino acids. The statistical modeling also indicated that botanical origin information is very poor in the NMR profiles of the analyzed wheat samples.

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“…Spectroscopic methods (NMR and UV) have successfully been applied in many earlier kinetic studies also related to lactone formation reactions within various compounds [11][12][13][14]. In addition, both qualitative and quantitative kinetic studies have been conducted to gain information on complex reacting multicomponent mixtures by utilizing NMR spectroscopy [15].…”

Section: Introductionmentioning

confidence: 99%

Intramolecular Lactonization of Poly(α-hydroxyacrylic acid): Kinetics and Reaction Mechanism

Virkki

Vuori

Vuorinen

2015

Journal of Polymers

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Poly( -hydroxyacrylic acid), PHA, is one of the few polymers with biodegradable properties used in mechanical pulp bleaching to stabilize hydrogen peroxide. A new method for the in situ follow-up of the lactone ring formation of PHA has been developed. The results have further been applied to describe the reaction kinetics of the lactonization and hydrolysis reactions through parameter estimation. In addition, the reaction mechanism is elucidated by multivariate data analysis. Satisfactory identification and semiquantitative separation of the lactone ring as well as the acyclic (carboxyl and hydroxyl groups) forms have been established by 1 H NMR in the pH range of 1-9. The lactonization reaction approaching equilibrium can be described by pseudo-first-order kinetics in the pH range of 1-6. The rate constants of the pseudo-first-order kinetic model have been estimated by nonlinear regression. Due to the very low rates of lactonization as well as the weak pH dependency of the reaction, an addition-elimination mechanism is proposed. Additionally, the presence of a transient reaction intermediate during lactonization reaction could be identified by subjecting the measurement data to multivariate data analysis (PCA, principal component analysis). A good correlation was found between the kinetic and the PCA models in terms of model validity.

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Wheat Flour Enzymatic Amylolysis Monitored by in Situ¹H NMR Spectroscopy

Cited by 30 publications

References 12 publications

Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions

Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions

Geographical origin of durum wheat studied by ¹H‐NMR profiling

Intramolecular Lactonization of Poly(α-hydroxyacrylic acid): Kinetics and Reaction Mechanism

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Wheat Flour Enzymatic Amylolysis Monitored by in Situ1H NMR Spectroscopy

Cited by 30 publications

References 12 publications

Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions

Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon Interactions

Geographical origin of durum wheat studied by 1H‐NMR profiling

Intramolecular Lactonization of Poly(α-hydroxyacrylic acid): Kinetics and Reaction Mechanism

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Wheat Flour Enzymatic Amylolysis Monitored by in Situ¹H NMR Spectroscopy

Geographical origin of durum wheat studied by ¹H‐NMR profiling