Wheat expansin gene TaEXPA2 is involved in conferring plant tolerance to Cd toxicity

Ren, Yuanqing; Chen, Yanhui; An, Jie; Zhao, Zhongxian; Zhang, Guangqiang; Wang, Yong; Wang, Wei

doi:10.1016/j.plantsci.2018.02.022

Cited by 45 publications

(40 citation statements)

References 51 publications

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“…Under Cd stress, TaEXPA2 conferred improved germination rate, root elongation, biomass accumulation, and photosynthesis when overexpressed in tobacco. The transgenic lines exhibited an efficient efflux of Cd, lower Cd accumulation, and enhanced antioxidant enzyme activities in comparison with the wild type [68]. These results suggested that TaEXPA2 may regulate plant Cd tolerance, probably through the activation of Cd efflux and antioxidation.…”

Section: Other Mechanismsmentioning

confidence: 77%

See 1 more Smart Citation

Breeding Low-Cadmium Wheat: Progress and Perspectives

Zaid

Zheng

2018

Agronomy

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Farmland cadmium (Cd) contamination has adverse impacts on both wheat grain yield and people’s well-being through food consumption. Safe farming using low-Cd cultivars has been proposed as a promising approach to address the farmland Cd pollution problem. To date, several dozen low-Cd wheat cultivars have been screened worldwide based on a Cd inhibition test, representing candidates for wheat Cd minimization. Unfortunately, the breeding of low-Cd wheat cultivars with desired traits or enhanced Cd exclusion has not been extensively explored. Moreover, the wheat Cd inhibition test for variety screening and conventional breeding is expensive and time-consuming. As an alternative, low-Cd wheat cultivars that were developed with molecular genetics and breeding approaches can be promising, typically by the association of marker-assisted selection (MAS) with conventional breeding practices. In this review, we provide a synthetics view of the background and knowledge basis for the breeding of low-Cd wheat cultivars.

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Section: Other Mechanismsmentioning

confidence: 77%

“…Ren et al reported a wheat expansin gene, TaEXPA2, which was upregulated in leaves when subjected to Cd stress [68]. Expansins are cell wall-located proteins that regulate plant development and response to various abiotic stresses [69][70][71][72].…”

Section: Other Mechanismsmentioning

confidence: 99%

Breeding Low-Cadmium Wheat: Progress and Perspectives

Zaid

Zheng

2018

Agronomy

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“…EXPAs may work in conjunction with cellulase and pectin lyase to break down CWs (Medina-Escobar et al, 1997;Llop-Tous et al, 1999;Harrison et al, 2001), although the exact mechanism(s) whereby EXPAs act on Cd is unclear. Previous findings showed that PtoEXPA12 over-expression (OX) increased Cd accumulation in tobacco plants (Zhang H. et al, 2018), although another study showed that TaEXPA2 OX is involved in tobacco resistance to Cd (Ren et al, 2018). After EXPA10 was knocked out, aluminum accumulation in the root hair region of rice plants decreased (Che et al, 2016).…”

Section: Discussionmentioning

confidence: 97%

Cell Wall Polysaccharide-Mediated Cadmium Tolerance Between Two Arabidopsis thaliana Ecotypes

Xiao

Liu

et al. 2020

Front. Plant Sci.

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Cadmium (Cd) is a toxic metal element and the mechanism(s) underlying Cd tolerance in plants are still unclear. Increasingly more studies have been conducted on Cd binding to plant cell walls (CW) but most of them have focused on Cd fixation by CW pectin, and few studies have examined Cd binding to cellulose and hemicellulose. Here we found that Cd binding to CW pectin, cellulose, and hemicellulose was significantly higher in Tor-1, a Cd tolerant A. thaliana ecotype, than in Ph2-23, a sensitive ecotype, as were the concentrations of pectin, cellulose, and hemicellulose. Transcriptome analysis revealed that the genes regulating CW pectin, cellulose, and hemicellulose polysaccharide concentrations in Tor-1 differed significantly from those in Ph2-23. The expressions of most genes such as pectin methyl esterase inhibitors (PMEIs), pectin lyases, xyloglucan endotransglucosylase/hydrolase, expansins (EXPAs), and cellulose hydrolase were higher in Ph2-23, while the expressions of cellulose synthase-like glycosyltransferase 3 (CSLG3) and pectin ethyl esterase 4 (PAE4) were higher in Tor-1. The candidate genes identified here seem to regulate CW Cd fixation by polysaccharides. In conclusion, an increase in pectin demethylation activity, the higher concentration of cellulose and hemicellulose, regulated by related genes, in Tor-1 than in Ph2-23 are likely involved in enhanced Cd CW retention and reduce Cd toxicity.

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“…Common wheat ( Triticum aestivum L.) is one of the most important food crops around the world, and the growth and yield of wheat are affected by various environmental factors, such as heavy metals [ 37 ]. To date, some genes related to heavy metal tolerance have been reported in wheat, including TaVP1 [ 38 ], TaGolS3 [ 37 ], TaEXPA2 [ 39 ], TaHMA2 [ 40 ], TaPCS1 [ 41 ], TaCNR2 [ 42 ], and TaPCS1 [ 43 ]. In this study, genome-wide identification of the HIPP gene family in Triticeae species was performed by searching the published sequences.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Heavy-Metal-Associated Isoprenylated Plant Protein (HIPP) Gene Family from Triticeae Species

Zhang

Liu

et al. 2020

IJMS

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Heavy-metal-associated (HMA) isoprenylated plant proteins (HIPPs) only exist in vascular plants. They play important roles in responses to biotic/abiotic stresses, heavy-metal homeostasis, and detoxification. However, research on the distribution, diversification, and function of HIPPs in Triticeae species is limited. In this study, a total of 278 HIPPs were identified from a database from five Triticeae species, and 13 were cloned from Haynaldia villosa. These genes were classified into five groups by phylogenetic analysis. Most HIPPs had one HMA domain, while 51 from Clade I had two, and all HIPPs had good collinear relationships between species or subgenomes. In silico expression profiling revealed that 44 of the 114 wheat HIPPs were dominantly expressed in roots, 43 were upregulated under biotic stresses, and 29 were upregulated upon drought or heat treatment. Subcellular localization analysis of the cloned HIPPs from H. villosa showed that they were expressed on the plasma membrane. HIPP1-V was upregulated in H. villosa after Cd treatment, and transgenic wheat plants overexpressing HIPP1-V showed enhanced Cd tolerance, as shown by the recovery of seed-germination and root-growth inhibition by supplementary Cd. This research provides a genome-wide overview of the Triticeae HIPP genes and proved that HIPP1-V positively regulates Cd tolerance in common wheat.

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Wheat expansin gene TaEXPA2 is involved in conferring plant tolerance to Cd toxicity

Cited by 45 publications

References 51 publications

Breeding Low-Cadmium Wheat: Progress and Perspectives

Breeding Low-Cadmium Wheat: Progress and Perspectives

Cell Wall Polysaccharide-Mediated Cadmium Tolerance Between Two Arabidopsis thaliana Ecotypes

Characterization of the Heavy-Metal-Associated Isoprenylated Plant Protein (HIPP) Gene Family from Triticeae Species

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