Wheat Cultivar Identification by Gliadin Electrophoregrams. I. Apparatus, Method and Nomenclature

Bushuk, W.; Zillman, R. R.

doi:10.4141/cjps78-076

Cited by 262 publications

(120 citation statements)

References 7 publications

(3 reference statements)

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“…The wheat samples used in this study were Electrophoregrams were determined as described provided by the Research Branch ofAgriculture previously (Bushuk and Zillman 1978). Three Canada.…”

mentioning

confidence: 99%

Wheat Cultivar Identification by Gliadin Electrophoregrams. Iii. Catalogue of Electrophoregram Formulas of Canadian Wheat Cultivars

Zillman¹,

Bushuk²

1979

Can. J. Plant Sci.

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Gliadin electrophoregram formulas of 88 common and durum wheat cultivars which had been licenced far production in Canada were determined and catalogued. The relative band mobilities, used to derive the formula, were calculated from actual band mobilities obtained from a photographic negative of a stained gel with a microcomparator. It was shown that band mobility was essentially constant across any one gel (i.e. gel polymerization was uniform) and reproducible to within ± 0.5 relative mobility unit from one gel to another (i.e. gel polymerization and electrophoresis were highly reproducible). A subjective quantification of band intensities is described. For the 88 cultivars examined. 85 distinct electrophoregram formulas were obtained. Three pairs of formulas were essentially identical. Each pair represented cultivars that were very closely related genetically. The use of the electrophoretic test and the catalogue of cultivar formulas for identifying unknown grain samples is discussed.

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“…The wheat samples used in this study were Electrophoregrams were determined as described provided by the Research Branch ofAgriculture previously (Bushuk and Zillman 1978). Three Canada.…”

mentioning

confidence: 99%

Wheat Cultivar Identification by Gliadin Electrophoregrams. Iii. Catalogue of Electrophoregram Formulas of Canadian Wheat Cultivars

Zillman¹,

Bushuk²

1979

Can. J. Plant Sci.

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“…Similar results (using the same solvents) were obtained in a previous study using barley cultivars (De Villiers & Laubscher, 1989). It was also found (results not shown) that extraction of gliadin proteins for only 1 h as used by Bushuk & Zillman (1978), Lookhart et al, (1982) and Khan, Hamada & Patek (1985) resulted in a loss of intensity of bands as well as a loss of faint 6 7 8 9…”

Section: Resultsmentioning

confidence: 62%

Wheat cultivar identification by electrophoretic analysis of gliadin proteins

Villiers

Bosman

1993

South African Journal of Plant and Soil

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Polyacrylamide gel electrophoresis of gliadin proteins in an acid medium was used to identify different spring wheat cultivars. Gliadins were extracted from single kernels or corresponding flour samples using different solvents. Gliadin electrophoretogram formulae using relative band mobilities and staining intensities were determined for each cultivar. Distinctive band patterns were obtained enabling the cultivars to be identified. Single kernels and flour samples gave identical band patterns differing only in the intensity of the staining of the bands.Poliakrielamiedgel-elektroforese van gliadienprote'iene in 'n suurmedium is gebruik om verskillende lentekoringcultivars te identifiseer. Gliadiene is uit enkel korrels of ooreenstemmende meelmonsters met verskillende oplosmiddels geekstraheer. Gliadien-elektroforetogram formules is vir elke cultivar bereken deur die relatiewe bandmobiliteit en kleurintensiteit van gliadienbande te gebruik. Kenmerkende bandpatrone is verkry waardeur cultivars ge"identifiseer kon word. Identiese bandpatrone is met korrels en meelmonsters verkry wat slegs in die intensiteit van die kleuring van die bande verskil het.

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“…ld) were inferior to those obtained for unalkylated hordein extracts (Fig. la) Bushuk and Zillman (1978) …”

Section: Alkylation Of Hordein Proteinsmentioning

confidence: 80%

Barley Cultivar Identification by Electrophoretic Analysis of Hordein Proteins: I. Extraction and Separation of Hordein Proteins and Environmental Effects on the Hordein Electrophoregram

Marchylo¹,

LaBerge²

1980

Can. J. Plant Sci.

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Wheat Cultivar Identification by Gliadin Electrophoregrams. I. Apparatus, Method and Nomenclature

Cited by 262 publications

References 7 publications

Wheat Cultivar Identification by Gliadin Electrophoregrams. Iii. Catalogue of Electrophoregram Formulas of Canadian Wheat Cultivars

Wheat Cultivar Identification by Gliadin Electrophoregrams. Iii. Catalogue of Electrophoregram Formulas of Canadian Wheat Cultivars

Wheat cultivar identification by electrophoretic analysis of gliadin proteins

Barley Cultivar Identification by Electrophoretic Analysis of Hordein Proteins: I. Extraction and Separation of Hordein Proteins and Environmental Effects on the Hordein Electrophoregram

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