Wheat CBL-interacting protein kinase 23 positively regulates drought stress and ABA responses

Abstract

BackgroundThe calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) signaling pathway responds to various abiotic stresses in plants.ResultsWheat CIPK23, isolated from wheat drought transcriptome data set, was induced by multiple abiotic stresses, including drought, salt, and abscisic acid (ABA). Compared with wild-type plants, TaCIPK23-overexpression wheat and Arabidopsis showed an higher survival rate under drought conditions with enhanced germination rate, developed root system, increased accumulation of osmolytes, and reduced water loss rate. Over-expression of TaCIPK23 rendered transgenic plants ABA sensitivity, as evidenced by delayed seed germination and the induction of stomatal closure. Consistent with the ABA-sensitive phenotype, the expression level of drought- and ABA-responsive genes were increased under drought conditions in the transgenic plants. In addition, using yeast two-hybrid system, pull-down and bimolecular fluorescence complementation (BiFc) assays, TaCIPK23 was found to interact with TaCBL1 on the plasma membrane.ConclusionsThese results suggest that TaCIPK23 plays important roles in ABA and drought stress responses, and mediates crosstalk between the ABA signaling pathway and drought stress responses in wheat.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1306-5) contains supplementary material, which is available to authorized users.

Integrated mRNA and miRNA transcriptome analysis reveals a regulatory network for tuber expansion in Chinese yam (Dioscorea opposita)

“…Interesting, CBLs were down-regulated (Additional file 3: Table S2). The role of CBL in root development is characterized by a robust gene expression during cell proliferation in the root, and its expression in root is regulated by auxin and cytokinin [47,48]. These results demonstrated that calcium signal is regulated during tuber expansion stage.…”

The CBL–CIPK Pathway in Plant Response to Stress Signals

“…Similar studies have not been reported in other species. In rice (Oryza sativa), wheat (Triticum aestivum), and poplar (Populus trichocarpa), OsCBL4, OsCBL8, and TaCBL1 are plasma membrane-localized proteins, while OsCBL2, OsCBL3, PtCBL10A, and PtCBL10B are localized to the tonoplast [83][84][85][86]. The localizations of most CBLs occur regularly, but some CBLs exhibit specificity that suggests localization but needs to be demonstrated in vivo.…”

“…Compared with CBLs, CIPKs are usually localized throughout the cytoplasmic and nuclear compartments without recognizable localization signals [13]. In wheat and pepper (Capsicum annuum), TaCIPK14, TaCIPK23, and CaCIPK1 are localized throughout the cell, and CaCIPK5 and CaCIPK20 are only found in the plasma membrane [34,84,87]. In most cases, the subcellular localizations of CBL-CIPK have been demonstrated by bimolecular fluorescence complementation, revealing that CBLs target CIPKs at the plasma membrane or tonoplast to fulfill their functions.…”

Genetic components of root architecture and anatomy adjustments to water‐deficit stress in spring barley

“…Reports have also shown that the overexpression of SAUR under salt and drought results in higher root length, survival rate, and improved drought/salt tolerance in Arabidopsis plants (Guo et al, ). The PBS gene families positively regulate drought stress in plants via ABA pathways, stomatal responses, and root growth (Wang et al, ; Cui et al, ). Two loci at 132.58 cM on 2H coding for RBG1 and at 69.11 cM on 7H interacted epistatically with each other.…”

“…ABA pathways, stomatal responses, and root growth (Wang et al, 2016;Cui et al, 2018). Two loci at 132.58 cM on 2H coding for RBG1 and at 69.11 cM on 7H interacted epistatically with each other.…”