Wheat AGAMOUS LIKE 6 transcription factors function in stamen development by regulating the expression of <i>Ta APETALA3</i>

Su, Yali; Liu, Jin-Xing; Liang, Wanqi; Dou, Yanhua; Fu, Ruifeng; Li, Wenqiang; Feng, Cui-Zhu; Gao, Caixia; Zhang, Dabing; Kang, Zhensheng; Li, Haifeng

doi:10.1242/dev.177527

Cited by 21 publications

(26 citation statements)

References 55 publications

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“…While the tissue expression specificity between branch meristems and inflorescence meristems was not established in these experiments, the dynamic gene expression profile in inflorescence and developing floral tissues support its role in Tripidium floral determinism [ 55 , 83 , 84 ]. In wheat, the TaAGL6 acts on staminate floral development by working on TaAPETALA3 [ 85 ]. In Tripidium , the homeotic activity of staminate floral development could be identified by the dramatic increase in transcript abundance of floral homeotic protein AG -like in samples at 80 cm of growth.…”

Section: Discussionmentioning

confidence: 99%

Reproductive developmental transcriptome analysis of Tripidium ravennae (Poaceae)

et al. 2021

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Background Tripidium ravennae is a cold-hardy, diploid species in the sugarcane complex (Poaceae subtribe Saccharinae) with considerable potential as a genetic resource for developing improved bioenergy and ornamental grasses. An improved understanding of the genetic regulation of reproductive processes (e.g., floral induction, inflorescence development, and seed development) will enable future applications of precision breeding and gene editing of floral and seed development. In particular, the ability to silence reproductive processes would allow for developing seedless forms of valuable but potentially invasive plants. The objective of this research was to characterize the gene expression environment of reproductive development in T. ravennae. Results During the early phases of inflorescence development, multiple key canonical floral integrators and pathways were identified. Annotations of type II subfamily of MADS-box transcription factors, in particular, were over-represented in the GO enrichment analyses and tests for differential expression (FDR p-value < 0.05). The differential expression of floral integrators observed in the early phases of inflorescence development diminished prior to inflorescence determinacy regulation. Differential expression analysis did not identify many unique genes at mid-inflorescence development stages, though typical biological processes involved in plant growth and development expressed abundantly. The increase in inflorescence determinacy regulatory elements and putative homeotic floral development unigenes at mid-inflorescence development coincided with the expression of multiple meiosis annotations and multicellular organism developmental processes. Analysis of seed development identified multiple unigenes involved in oxidative-reductive processes. Conclusion Reproduction in grasses is a dynamic system involving the sequential coordination of complex gene regulatory networks and developmental processes. This research identified differentially expressed transcripts associated with floral induction, inflorescence development, and seed development in T. ravennae. These results provide insights into the molecular regulation of reproductive development and provide a foundation for future investigations and analyses, including genome annotation, functional genomics characterization, gene family evolutionary studies, comparative genomics, and precision breeding.

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Section: Discussionmentioning

confidence: 99%

Reproductive developmental transcriptome analysis of Tripidium ravennae (Poaceae)

et al. 2021

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“…Transcription factors play an important role in regulating the development of plants. Some previous studies have reported that MADS-box [9,14,16,17,26,27,28] and DROOPING LEAF gene of YABBY family [6,15,16] associated with the development of wheat pistillody-stamen. We identify MADS-box and YABBY transcription factors from wheat flower substructure tissues (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Co-expression network analysis of the genes associated with pistillody-stamen development in wheat

Liao¹,

Chen²,

Yang³

et al. 2020

Preprint

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Background: Crop male sterility has great values in both theoretical research and breeding application. Wheat pistillody-stamen is an important male sterility phenomenon, and HTS-1 is an important pistillody-stamen material. However the molecular mechanism of HTS-1 stamens transformed into pistils or pistil-like structures remains a mystery. Weighted gene co-expression network analysis (WGCNA) are widely used to explore hub genes and gene interaction networks from high throughput data in various plants. Results: In the present study, for exploring gene networks associated with wheat pistillody-stamen development, WGCNA was employed to analyze 11 RNA-sequencing (RNA-seq) data of wheat tissues, including stamens of CSTP, pistils and pistillody-stamen of HTS-1. 19 out of 25 merged modules were highly associated with specific wheat tissues, and the MEdarkseagreen1 module was highly related to wheat pistillody-stamen (correlation with weight r =0.7, correlation p-value p =0.02). Then 180 genes about wheat flower development were identified from the MEdarkseagreen1 module by GO term analysis. Among 180 genes, the hub gene number associated with anther, filament, style, and ovary development were 12, 3, 3, and 10, respectively. We compared the published pistillody related proteins with proteins of HTS-1 by BLAST. A total of 58 pistillody-stamen development associated proteins were validated by BLAST. MADS-box and YABBY transcription factor about pistillody-stamen development were also analyzed in wheat flower. There were 47 of MADS-box and 17 of YABBY transcription factors were identified. BLAST program was used to align the published pistillody associated MADS-box and YABBY transcription factors with transcription factors identified in wheat flower. Totally, 36 of 47 MADS-box and 14 of 17 YABBY transcription factors were considered to regulate the development of pistillody-stamen, which had never been reported yet. Conclusion: These results have systematically identified the key candidate genes about the development of HTS-1 substructures flower. The tissue-specific correlation network analyses provide important insights into the molecular interactions underlying psitillody-stamen development.

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“…Mutation of any of the key genes will lead to male sterility. Some reports have demonstrated that mutations of the genes critical for anther and pollen development will result in male sterility [30,31,[34][35][36][37][38]67]. In this study, the metabolic pathways related to male sterility, such as phenylalanine metabolism (ko00360), phenylpropanoid biosynthesis (ko00940), and glutathione metabolism (ko00480), were identified via K-means analysis.…”

Section: Complex Network Regulating Anther Development Of Nwms1mentioning

confidence: 97%

“…Wheat AGL6 transcription factors interact with TaAP3, TaAGAMOUS, and TaMADS13. TaAGL6 plays an essential role in stamen development through transcriptional regulation of TaAP3 and other related genes [38]. These researches have enriched the mechanism study of wheat male sterility.Although plant male development mechanisms are similar, because of the diverse floral phenotypes of various species, the molecular mechanism of wheat male fertility is unique and complicated, and there are many issues that need to be elucidated.…”

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confidence: 98%

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The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

Zhang

et al. 2019

IJMS

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Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.which is one of the key ways to improve wheat yield [6]. Male sterility permits the production of hybrids on a commercial scale, relying on heterosis in crops, and can greatly increase selection efficiency of the yield [7]. In-depth understanding of the molecular mechanism of male sterility can help us to use it more effectively.Plant male sterility was first reported in the 18th century [8]. A large number of plant male sterility mutants have been reported over the past few decades. Male sterility includes cytoplasmic male sterility (CMS) controlled by mitochondrial genes coupled with nuclear genes, and genic male sterility (GMS) controlled by nuclear genes alone [9][10][11]. The pistils of the cytoplasmic male sterile lines and genic male sterile lines are normal, but their stamens are abnormal and cannot pollinate normally, which is due to stamen degeneration, pollen abortion, or functional sterility. GMS occurs in 216 species and 17 species crosses, while CMS occurs in more than 150 species and 271 species crosses [11][12][13]. Plant male sterility lines have been widely studied and applied successfully in many crops, such as maize (Zea mays), rice (Oryza sativa), soybean (Glycine max), and barley (Hordeum vulgare). Several wheat CMS lines have been studied and applied in production; however, commercial application is limited [14][15][16]. Possible applications of GMS in plant breeding have been reviewed and discussed for at least 30 years [17]. Up to now, only five GMS genes have been...

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Wheat AGAMOUS LIKE 6 transcription factors function in stamen development by regulating the expression of Ta APETALA3

Cited by 21 publications

References 55 publications

Reproductive developmental transcriptome analysis of Tripidium ravennae (Poaceae)

Reproductive developmental transcriptome analysis of Tripidium ravennae (Poaceae)

Co-expression network analysis of the genes associated with pistillody-stamen development in wheat

The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

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