What we can see from very small size sample of metagenomic sequences

Kwak, Jaesik; Park, Joonhong

doi:10.1186/s12859-018-2431-8

Cited by 6 publications

(4 citation statements)

References 22 publications

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“…Isolation efficiency can be enhanced by the use of microdroplet and microfluidics technologies adapted as high‐throughput culturing platforms (Kaminski et al ., 2016) to allow for large‐scale isolation, genome sequencing and functional screening of microbial isolates. To improve success of culturing previously uncultured microbiome members, single‐cell amplified (SAGs) and metagenome‐assembled (MAGs) genomes together with genome‐based metabolic reconstructions are being used to guide formulation of specific media recipes (Kwak & Park, 2018).…”

Section: Multiomics Approaches In Understanding Plant Microbiomesmentioning

confidence: 99%

Enabling sustainable agriculture through understanding and enhancement of microbiomes

et al. 2021

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Summary Harnessing plant‐associated microbiomes offers an invaluable strategy to help agricultural production become more sustainable while also meeting growing demands for food, feed and fiber. A plethora of interconnected interactions among the host, environment and microbes, occurring both above and below ground, drive recognition, recruitment and colonization of plant‐associated microbes, resulting in activation of downstream host responses and functionality. Dissecting these complex interactions by integrating multiomic approaches, high‐throughput culturing, and computational and synthetic biology advances is providing deeper understanding of the structure and function of native microbial communities. Such insights are paving the way towards development of microbial products as well as microbiomes engineered with synthetic microbial communities capable of delivering agronomic solutions. While there is a growing market for microbial‐based solutions to improve crop productivity, challenges with commercialization of these products remain. The continued translation of plant‐associated microbiome knowledge into real‐world scenarios will require concerted transdisciplinary research, cross‐training of a next generation of scientists, and targeted educational efforts to prime growers and the general public for successful adoption of these innovative technologies.

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Section: Multiomics Approaches In Understanding Plant Microbiomesmentioning

confidence: 99%

Enabling sustainable agriculture through understanding and enhancement of microbiomes

et al. 2021

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“…The cultured members enable validate candidate genes by molecular approaches such as mutagenic and bioreporter expression systems ( Cole et al, 2017 ; Pini et al, 2017 ). In order to improve the culturing of previously uncultured microbes, single-cell amplified and metagenome-assembled genomes integrated with genome-based metabolic constructions are used for the formulation of specific media recipes ( Kwak and Park, 2018 ). Summarized studies ( Figure 2 ) have highlighted the important steps involved in investigations on the role of citrus microbiome influencing both soil health and plant health.…”

Section: Multiomics For Decoding Plant Microbiomesmentioning

confidence: 99%

Bioprospecting Microbiome for Soil and Plant Health Management Amidst Huanglongbing Threat in Citrus: A Review

et al. 2022

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Microorganisms have dynamic and complex interactions with their hosts. Diverse microbial communities residing near, on, and within the plants, called phytobiome, are an essential part of plant health and productivity. Exploiting citrus-associated microbiomes represents a scientific approach toward sustained and environment-friendly module of citrus production, though periodically exposed to several threats, with Huanglongbing (HLB) predominantly being most influential. Exploring the composition and function of the citrus microbiome, and possible microbial redesigning under HLB disease pressure has sparked renewed interest in recent times. A concise account of various achievements in understanding the citrus-associated microbiome, in various niche environments viz., rhizosphere, phyllosphere, endosphere, and core microbiota alongside their functional attributes has been thoroughly reviewed and presented. Efforts were also made to analyze the actual role of the citrus microbiome in soil fertility and resilience, interaction with and suppression of invading pathogens along with native microbial communities and their consequences thereupon. Despite the desired potential of the citrus microbiota to counter different pathogenic diseases, utilizing the citrus microbiome for beneficial applications at the field level is yet to be translated as a commercial product. We anticipate that advancement in multiomics technologies, high-throughput sequencing and culturing, genome editing tools, artificial intelligence, and microbial consortia will provide some exciting avenues for citrus microbiome research and microbial manipulation to improve the health and productivity of citrus plants.

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“…In this study, we will include 10 patients in each group to do the metagenomic analysis. This might be a small sample size compared to the clinical studies, which may influence the differences among the groups, but considerations of cost and reimbursement and small size of sample size for microbial studies may be feasible [73].…”

Section: Discussionmentioning

confidence: 99%

Differences in the individual curative effect of acupuncture for obese women with polycystic ovary syndrome based on metagenomic analysis: study protocol for a randomized controlled trial

Fan

Xia

Zeng

et al. 2021

Trials

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Background Polycystic ovary syndrome is a common cause of infertility and shows a high incidence in women of reproductive age. Acupuncture is an appropriate adjunctive treatment for PCOS. However, the add-on effect of acupuncture as an adjunctive treatment for obese women with PCOS has not been studied, and previous studies indicate that there are individual differences in the curative effect of acupuncture, while deeper research on the mechanism of differences in the individual curative effect of acupuncture for obese women with PCOS is still lacking. This trial aims to assess the add-on treatment efficacy of acupuncture for obese women with PCOS and to explore the role of the gut microbiome on the differences in the individual curative effect of acupuncture based on metagenomic analysis. Methods/design This is an open-label, randomized, controlled trial. A total of 86 obese women with PCOS will be recruited. Subjects will be randomly assigned to a study group and a control group in a 1:1 ratio, with 43 subjects in each group (10 patients from each group who meet the study criteria will participate in the metagenomic analysis). An additional 10 subjects who meet the study criteria will be recruited to a healthy control group. The study group will receive acupuncture and clomiphene citrate treatment; the control group will only receive clomiphene citrate. Acupuncture treatment will be conducted three times a week from the fifth day of menstruation or withdrawal bleeding until the start of the next menstruation, for up to three menstrual cycles. The primary outcome will be LH/FSH. The secondary outcomes will comprise biometric features, hormone biomarkers, metabolic biomarkers, inflammatory biomarkers, Self-Rating Anxiety Scale, Self-Rating Depression Scale, and metagenomic analysis. The outcomes will be measured at baseline and post-intervention. Data will be analyzed using SPSS 19.0, and the gut microbiome will be analyzed using metagenomic analysis. Discussion In this study, we are evaluating the add-on effects of acupuncture and exploring the mechanism of the differences in the individual curative effect of acupuncture based on the gut microbiome, which may provide evidence to explain the different outcomes of different trials on acupuncture for PCOS and hopefully to provide a new aspect to study the mechanism of acupuncture’s treatment effect. Trial registration Chinese Clinical Trial Registry ChiCTR2000029882. Registered on 16 February 2020

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What we can see from very small size sample of metagenomic sequences

Cited by 6 publications

References 22 publications

Enabling sustainable agriculture through understanding and enhancement of microbiomes

Enabling sustainable agriculture through understanding and enhancement of microbiomes

Bioprospecting Microbiome for Soil and Plant Health Management Amidst Huanglongbing Threat in Citrus: A Review

Differences in the individual curative effect of acupuncture for obese women with polycystic ovary syndrome based on metagenomic analysis: study protocol for a randomized controlled trial

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