What to do in the event of a suspicion of analytical interference during an immunoassay?

Lauro, Cindy; Corcuff, Jean-Benoı̂t; Brossaud, Julie; Georges, A.

doi:10.1684/abc.2020.1514

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Their impacts in immunoassays are diverse, either giving high, or falsely low results, depending on the assay design and epitopes recognition [7]. Dilution tests, HBT pretreatment and comparison with alternative assays, are common tools to evidence an erroneous result, provided that a clinical suspicion is raised [8].…”

mentioning

confidence: 99%

FGF23 measurement in burosumab-treated patients: an emerging treatment may induce a new analytical interference

Piketty

Souberbielle

Maruani

et al. 2020

Clinical Chemistry and Laboratory Medicine (CCLM)

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mentioning

confidence: 99%

FGF23 measurement in burosumab-treated patients: an emerging treatment may induce a new analytical interference

Piketty

Souberbielle

Maruani

et al. 2020

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Strategies and algorithms have been defined, either in general or dedicated to thyroid function, Tg, or PTH assays [2, 31, 75, [31,170]. Each laboratory must validate the interference testing protocol for each immunoassay, as some methods are not valid after HBT or PEG pretreatment [173].…”

Section: Solutions In the Face Of Suspected Interferencementioning

confidence: 99%

Hormone Immunoassay Interference: A 2021 Update

et al. 2022

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Immunoassays are powerful qualitative and quantitative analytical techniques. Since the first description of an immunoassay method in 1959, advances have been made in assay designs and analytical characteristics, opening the door for their widespread implementation in clinical laboratories. Clinical endocrinology is closely linked to laboratory medicine because hormone quantification is important for the diagnosis, treatment, and prognosis of endocrine disorders. Several interferences in immunoassays have been identified through the years; although some are no longer encountered in daily practice, cross-reaction, heterophile antibodies, biotin, and anti-analyte antibodies still cause problems. Newer interferences are also emerging with the development of new therapies. The interfering substance may be exogenous (e.g., a drug or substance absorbed by the patient) or endogenous (e.g., antibodies produced by the patient), and the bias caused by interference can be positive or negative. The consequences of interference can be deleterious when clinicians consider erroneous results to establish a diagnosis, leading to unnecessary explorations or inappropriate treatments. Clinical laboratories and manufacturers continue to investigate methods for the detection, elimination, and prevention of interferences. However, no system is completely devoid of such incidents. In this review, we focus on the analytical interferences encountered in daily practice and possible solutions for their detection or elimination.

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Interferences in immunoassays: review and practical algorithm

Wauthier

Plebani

Favresse

2022

Clinical Chemistry and Laboratory Medicine (CCLM)

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Immunoassays are currently the methods of choice for the measurement of a large panel of complex and heterogenous molecules owing to full automation, short turnaround time, high specificity and sensitivity. Despite remarkable performances, immunoassays are prone to several types of interferences that may lead to harmful consequences for the patient (e.g., prescription of an inadequate treatment, delayed diagnosis, unnecessary invasive investigations). A systematic search is only performed for some interferences because of its impracticality in clinical laboratories as it would notably impact budget, turnaround time, and human resources. Therefore, a case-by-case approach is generally preferred when facing an aberrant result. Hereby, we review the current knowledge on immunoassay interferences and present an algorithm for interference workup in clinical laboratories, from suspecting their presence to using the appropriate tests to identify them. We propose an approach to rationalize the attitude of laboratory specialists when faced with a potential interference and emphasize the importance of their collaboration with clinicians and manufacturers to ensure future improvements.

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What to do in the event of a suspicion of analytical interference during an immunoassay?

Abstract: Conduite à tenir devant une suspicion d'interférence analytique lors d'un immunodosageWhat to do in the event of a suspicion of analytical interference during an immunoassay?

Cited by 3 publications

References 5 publications

FGF23 measurement in burosumab-treated patients: an emerging treatment may induce a new analytical interference

FGF23 measurement in burosumab-treated patients: an emerging treatment may induce a new analytical interference

Hormone Immunoassay Interference: A 2021 Update

Interferences in immunoassays: review and practical algorithm

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