What's New in the Ultrastructure of Tumor Invasion in vivo?

Dingemans, K. P.

doi:10.1016/s0344-0338(88)80071-2

Cited by 22 publications

(7 citation statements)

References 109 publications

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“…Conversely, traces of basement membrane could be found even in the most irregular, apparently invasive tumor-cell groups. We have previously reported that in human material tumor cells, while penetrating, eg., arterial walls, may have a basement membrane (Dingemans, 1988). Such observations lend further support to the growing body of evidence that the relation between basement-membrane loss and invasiveness is not a straightforward one (Gusterson et al, 1984;Dingemans, 1988).…”

Section: Discussionsupporting

confidence: 60%

Transplantation of colon carcinoma into granulation tissue induces an invasive morphotype

Dingemans

Zeeman-Boeschoten

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et al. 1993

Intl Journal of Cancer

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The stroma surrounding many malignant tumors resembles granulation tissue. To test the hypothesis that such stroma stimulates tumor invasiveness, we compared, by electron microscopy and immunohistochemistry, the growth patterns of CC531 rat colon adenocarcinoma in 2 experimental situations: (i) after transplantation into the undisturbed subcutaneous connective tissue of rats, and (ii) after transplantation into experimentally induced subcutaneous granulation tissue in rats. For the latter experimental situation, a subcutaneous "tissue chamber" was designed allowing fragments of tumor tissue to be transplanted into the very center of developing granulation tissue. In the undisturbed subcutaneous tissue, the whole tumor was generally encapsulated, and the tumor cells were arranged in compact groups with a strong tendency to form acini. In the pre-formed granulation tissue, on the other hand, the tumor tissue closely matched descriptions of invasive colon carcinomas in the literature and met the criteria for the "invasive morphotype". In this situation, the tumor consisted of thin, unorganized, widely dispersed strands of irregular tumor cells with numerous protrusions that deeply penetrated the surrounding matrix. Our results show that an invasive morphotype can be evoked by pre-inducing granulation tissue at the transplantation site.

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Section: Discussionsupporting

confidence: 60%

Transplantation of colon carcinoma into granulation tissue induces an invasive morphotype

Dingemans

Zeeman-Boeschoten

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et al. 1993

Intl Journal of Cancer

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“…Many evidence indicated that actin filaments played an important role in promoting cell invasion [27, 28]. The activation of JNK pathway was known to be involved in modulating cytoskeleton like actin filaments [29, 30].…”

Section: Resultsmentioning

confidence: 99%

Dickkopf-4 is frequently overexpressed in epithelial ovarian carcinoma and promotes tumor invasion

Wang¹,

Wei²,

Zhang³

2017

BMC Cancer

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BackgroundDickkopf-4 (DKK4), a member of DKK family, appears to be a divergent protein. It remained multi-biological functions in carcinogenesis. The effect of DKK4 on the ovarian cancer cells remains unclear. This study detected the clinical significance of DKK4 in epithelial ovarian cancer (EOC) patients and its role in invasion.MethodsQRT-PCR and western blot analysis were used to examine the levels of DKK4 mRNA and protein in 33 EOC tissues and 33 benign ovarian tumors. Immunohistochemical analysis was performed to assess DKK4 expression in 239 EOC samples. siRNA-mediated DKK4 silence was conducted. Transwell assay was used to detect the invasive ability. Phalloidin was used to stain the formations of actin filaments.ResultsThe expressions of DKK4 mRNA and protein were elevated in EOC tissues as compared with those in benign ovarian tumors (p = 0.001 and <0.0001 respectively). Immunohistochemical results showed the strong expression of DKK4 protein was positively associated with late FIGO stage (p = 0.005) and poor disease free survival in univariate and multivariate analysis (p < 0.0001 and p = 0.001, respectively). SiRNA-mediated DKK4 knockdown inhibited cell invasive ability (all p < 0.0001) and the formations of actin filaments. DKK4 could promote the phosphration of c-JUN and JNK (p < 0.0001 and p = 0.001, respectively).ConclusionsOur results indicated that DKK4 might be contributed to predicting EOC progression and prognosis. DKK4 could promote the invasion of EOC through JNK activation.

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“…It is assumed that the BM around cancer cells is broken down in the active invasion stages and is reconstructed in a phase of temporary standstill of the invasive process. 21 In the well-differentiated carcinomas, cancer cell nests were frequently surrounded by continuous BM components, whereas a progressive lack of these components paralleled a decreasing degree of tumor differentiation. On the other hand, partial or widespread loss of BM components around carcinoma cell nests was frequently found in T1 or T2, as well as T3 or T4 tumors.…”

Section: Discussionmentioning

confidence: 94%

Immunohistologic distribution of basement membrane in oral squamous cell carcinoma

et al. 1994

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The distribution pattern of the basement membrane (BM) around tumor cells was determined in 72 oral squamous cell carcinomas by immunohistochemical staining with antibodies against human type IV collagen, laminin, and heparan sulfate proteoglycan. An intact continuous BM was found in 29 cases, whereas partial or widespread loss of the BM was detected in the other 43 cases (59.7%). Statistical analysis showed that the degree of BM loss was correlated with the degree of differentiation of tumor cells, but not with tumor size, and, most significantly, with the mode of cancer invasion and the incidence of lymph node metastasis. Carcinoma with a well-defined tumor-stromal boundary generally expressed an intact continuous BM. In contrast, the majority of diffusely invasive carcinomas lacked a continuous BM. Carcinomas with a widespread loss of BM structures showed a high frequency of regional lymph node metastasis (16 of 18 cases, 88.9%).

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What's New in the Ultrastructure of Tumor Invasion in vivo?

Cited by 22 publications

References 109 publications

Transplantation of colon carcinoma into granulation tissue induces an invasive morphotype

Transplantation of colon carcinoma into granulation tissue induces an invasive morphotype

Dickkopf-4 is frequently overexpressed in epithelial ovarian carcinoma and promotes tumor invasion

Immunohistologic distribution of basement membrane in oral squamous cell carcinoma

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