What's new in chitinase research?

Flach, Jean; Pilet, Paul‐Emile; Jollès, Pierre

doi:10.1007/bf02124285

Cited by 324 publications

(213 citation statements)

References 159 publications

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“…30,31 The microfilarial chitinase is an immunodominant antigen and target of current strategies to develop candidate vaccines. [32][33][34] The functional contribution of the microfilarial chitinase to infectious pathophysiology is still not well understood.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro, human chitotriosidase is capable of degrading chitincontaining pathogens. 26,30 Some authors have suggested that, on the basis of in vitro chitinase activity, it could play a role in microbicidal activity against complex pathogens, such as the filarial parasite whose microfilarial sheath contains chitin. 35 Recently, a mouse chitinaselike molecule (YM-1) has been shown to be highly expressed by filarial exposed alternatively activated macrophages (JE Allen, personal communication).…”

Section: Discussionmentioning

confidence: 99%

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Genetic polymorphisms in molecules of innate immunity and susceptibility to infection with Wuchereria bancrofti in South India

et al. 2001

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A pilot study was conducted to determine if host genetic factors influence susceptibility and outcomes in human filariasis.Using the candidate gene approach, a well-characterized population in South India was studied using common polymorphisms in six genes (CHIT1, MPO, NRAMP, CYBA, NCF2, and MBL2) . A total of 216 individuals from South India were genotyped; 67 normal (N), 63 asymptomatic microfilaria positive (MF+), 50 with chronic lymphatic dysfunction/elephantiasis (CP), and 36 tropical pulmonary eosinophilia (TPE). An association was observed between the HH variant CHIT1 genotype, which correlates with decreased activity and levels of chitotriosidase and susceptibility to filarial infection (MF+ and CP; P = 0.013). The heterozygosity of CHIT1 gene was over-represented in the normal individuals (P = 0.034). The XX genotype of the promoter region in MBL2 was associated with susceptibility to filariasis (P = 0.0093). Since analysis for MBL-sufficient vs insufficient haplotypes was not informative, it is possible the

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genetic polymorphisms in molecules of innate immunity and susceptibility to infection with Wuchereria bancrofti in South India

et al. 2001

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“…Chitinases are ubiquitous chitin-fragmenting hydrolases and produced by a vast array of organisms [5]. Recently, two functional chitinases, chitotriosidase and acidic mammalian chitinase (AMCase), have been identified in mammalian species [2,3,15].…”

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confidence: 99%

Quantitative Changes in Serum Concentration of Bovine Gut Chitinase in Theileria Infection

Fujimoto

Kimura

Suzuki

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. Bovine gut chitinase is exclusively produced in the liver and secreted into the blood. In the present study, we established a semi-quantitative method by Western blot analysis for measurement of the chitinase content in blood and examined its alteration during postnatal development and experimental infection with hemoprotozoan parasite in cattle. Its serum levels from 1 week to 1 year of age showed a significant increase only in 3-4-month-old group. The plasma concentration of the gut chitinase was not changed during acute inflammation caused by lipopolysaccharide but increased gradually after a Theileria injection and peaked at 52 days post-infection. It appears that the increase in the blood chitinase levels might be a defensive response in cattle against protozoan infection. KEY WORDS: bovine serum, chitinase, Theileria sergenti.

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“…A knowledge of parasite metabolism and its cytopathic effects are therefore of interest for the design of new drugs to control trichomoniasis. Chitinases are enzymes found in plants, insects and parasites (Flach et al, 1992). These enzymes cleave β-l->4 linkages between N-acetylglucosamine (GlcNAc) residues, releasing oligomers.…”

Section: Introductionmentioning

confidence: 99%

Chitinolytic activities inTrichomonas vaginalis

1998

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Summary :Chitinolytic activities were identified in the Protozoa Trichomonas vaginalis. Overall chitinase activity assessed using chitine-azure as substrate was 10.93 ± 1.21 nmoles/min/mg prot. End nonreducing chitobiosidase (exochitinase) and chitotriosidase (endochitinase) activities were shown using p-nitrophenyl-substrates and had specific activities of 4.55 ± 0.53 and 0.47 ± 0.06 nmol/min/mg prot, K m app.= 1.32 mM and 5 μM and pH optimum = 7.0 and 6.1 respectively. ß-N-acetylhexosaminidase (NAHase) activity was also detected with a specific activity of 5.40 ± 0.65 nmol/min/mg prot., K m app = 0.656 mM and pH optimum at 7.0. No release ol these enzymes into the culture medium was found. The possible role of chitinases in T. vaginalis remains to be investigated.

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What's new in chitinase research?

Cited by 324 publications

References 159 publications

Genetic polymorphisms in molecules of innate immunity and susceptibility to infection with Wuchereria bancrofti in South India

Genetic polymorphisms in molecules of innate immunity and susceptibility to infection with Wuchereria bancrofti in South India

Quantitative Changes in Serum Concentration of Bovine Gut Chitinase in Theileria Infection

Chitinolytic activities inTrichomonas vaginalis

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