What’s in the box? Authentication of Echinacea herbal products using DNA metabarcoding and HPTLC

Raclariu, Ancuta Cristina; Ţebrencu, Carmen Elena; Ichim, Mihael Cristin; Ciupercǎ, Oana Teodora; Brysting, Anne K.; Boer, Hugo de

doi:10.1016/j.phymed.2018.03.058

Cited by 51 publications

(49 citation statements)

References 35 publications

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“…Next-Generation Sequencing (NGS) technologies have been applied to herbal drug authentication using genome skimming or amplicon metabaroding approaches [ 1 , 8 , 87 , 88 , 89 , 90 , 91 , 92 ], including H. perforatum commercial products [ 16 ]. The quality of a herbal product is determined by the correct identity of its ingredients and its purity, measured by % contamination by a range of inorganic and biological contaminants.…”

Section: Discussionmentioning

confidence: 99%

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Howard

Lockie-Williams

Slater

2020

Plants

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DNA barcoding is a widely accepted technique for the identification of plant materials, and its application to the authentication of commercial medicinal plants has attracted significant attention. The incorporation of DNA-based technologies into the quality testing protocols of international pharmacopoeias represents a step-change in status, requiring the establishment of standardized, reliable and reproducible methods. The process by which this can be achieved for any herbal medicine is described, using Hypericum perforatum L. (St John’s Wort) and potential adulterant Hypericum species as a case study. A range of practical issues are considered including quality control of DNA sequences from public repositories and the construction of individual curated databases, choice of DNA barcode region(s) and the identification of informative polymorphic nucleotide sequences. A decision tree informs the structure of the manuscript and provides a template to guide the development of future DNA barcode tests for herbals.

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Section: Discussionmentioning

confidence: 99%

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Howard

Lockie-Williams

Slater

2020

Plants

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“…However, there are considerable limitations with NGS regarding its ability to generate scientifically valid test results. Due to its exceptional sensitivity, NGS detects the presence of DNA of minor, often inconsequential, contaminants, such as from pollen or a blade of grass (Raclariu et al 2018). Some industry analysts attempt to use NGS to provide quantitative data regarding the amount of DNA present in a sample with the premise that the majority of the DNA present will represent the majority of the sample.…”

Section: Genetic Assaysmentioning

confidence: 99%

Botanical ingredient identification and quality assessment: strengths and limitations of analytical techniques

Upton¹,

David

Gafner

et al. 2019

Phytochem Rev

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Interest in botanical medicines is increasing worldwide with current global market values estimated to be greater than US $72 billion. To ensure ingredients used in botanical preparations are appropriately identified and will deliver the intended benefit while minimizing potential for risk, good manufacturing practices (GMPs) are required. GMPs require that scientifically valid analytical techniques be used. This review discusses a variety of techniques of quality assessment taking into account the different challenges between academicians and manufacturers with the basic premise that all techniques are scientifically valid if used appropriately, and conversely, are limited if not used in a scientifically valid manner. The strengths and limitations, applications and inapplicability, of some of these techniques will be discussed, as will differences between traditional and more modern analytical techniques. A strong emphasis is placed on classical botanical and traditional macromorphological assessment techniques that represent the basis of identification and quality assessment upstream of other techniques such as analytical chemistry and genetics. It will be shown that it is the suite of botanical and chemical techniques that provides the greatest confidence for ensuring the identity and quality of botanical medicines.

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“…DNA metabarcoding was used to authenticate herbal products containing Echinacea (Raclariu et al 2018b), Hypericum (Raclariu et al 2017a) and Veronica (Raclariu et al 2017a) DNA metabarcoding of 78 herbal products containing Hypericum perforatum L. (St. John's wort) detected the target species in only 68% of products (Raclariu et al 2017b). Authentication of 16 herbal products containing Veronica officinalis L. (common speedwell) though DNA metabarcoding detected the target species in only 15% of the products, but detected a related species, V. chamaedrys (germander speedwell) as an undeclared ingredient in 62% of the products (Raclariu et al 2017a).…”

Section: Descriptionmentioning

confidence: 99%

“…Authentication of 16 herbal products containing Veronica officinalis L. (common speedwell) though DNA metabarcoding detected the target species in only 15% of the products, but detected a related species, V. chamaedrys (germander speedwell) as an undeclared ingredient in 62% of the products (Raclariu et al 2017a). Three common species of Echinacea were targeted in 53 herbal products and using DNA metabarcoding, Echinacea species were detected in 34 out of the 38 retained products (89%), but with a lack of discriminatory resolution at the species level (Raclariu et al 2018b). It should be noted that DNA barcoding and metabarcoding detected inconsistency between reported constituents and identified species in all analysed products.…”

Section: Descriptionmentioning

confidence: 99%

PhytoAuthent: Molecular authentication of complex herbal food supplements for safety and efficacy

Ichim¹,

Crișan²,

Ţebrencu³

et al. 2018

RIO

Self Cite

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The PhytoAuthent project was structured to gather, test, develop and apply, in real life case scenarios, molecular techniques, such as biochemical fingerprinting and DNA sequencebased methods, for plant identification of constituents in complex herbal products. The project had a strong focus on applied aspects like protecting consumers from health risks associated with product substitution and contamination of herbal products.

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What’s in the box? Authentication of Echinacea herbal products using DNA metabarcoding and HPTLC

Abstract: The results confirm that DNA metabarcoding can be used to test for the presence of Echinacea species and simultaneously to detect other species present in even highly processed and multi-ingredient herbal products.

Cited by 51 publications

References 35 publications

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Applied Barcoding: The Practicalities of DNA Testing for Herbals

Botanical ingredient identification and quality assessment: strengths and limitations of analytical techniques

PhytoAuthent: Molecular authentication of complex herbal food supplements for safety and efficacy

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