What’s in the box? Authentication of<i>Echinacea</i>herbal products using DNA metabarcoding and HPTLC

Raclariu, Ancuta Cristina; Ţebrencu, Carmen Elena; Ichim, Mihael Cristi; Ciupercǎ, Oana Teodora; Brysting, Anne K.; Boer, Hugo de

doi:10.1101/202721

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…Three common species of Echinacea were targeted in 53 herbal products and using DNA metabarcoding, Echinacea species were detected in 34 out of the 38 retained products (89%), but with a lack of discriminatory resolution at the species level (Raclariu et al 2017b). It should be noted that DNA barcoding and metabarcoding detected inconsistency between reported constituents and identified species in all analysed products.…”

Section: Descriptionmentioning

confidence: 99%

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PhytoAuthent: Molecular authentication of complex herbal food supplements for safety and efficacy

Ichim¹,

Crișan²,

Ţebrencu³

et al. 2017

RIO

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The PhytoAuthent project was structured to gather, test, develop and apply, in real life case scenarios, molecular techniques, such as biochemical fingerprinting and DNA sequencebased methods, for plant identification of constituents in complex herbal products. The project had a strong focus on applied aspects like protecting consumers from health risks associated with product substitution and contamination of herbal products.

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Section: Descriptionmentioning

confidence: 99%

“…DNA metabarcoding was used to authenticate herbal products containing Echinacea (Raclariu et al 2017b), Hypericum (Raclariu et al 2017c) and Veronica (Raclariu et al 2017d). DNA metabarcoding of 78 herbal products containing Hypericum perforatum L. (St. John's wort) detected the target species in only 68% of products (Raclariu et al 2017d).…”

Section: Descriptionmentioning

confidence: 99%

PhytoAuthent: Molecular authentication of complex herbal food supplements for safety and efficacy

Ichim¹,

Crișan²,

Ţebrencu³

et al. 2017

RIO

Self Cite

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“…Multi-locus DNA barcoding has been used in many studies for the authentication of species in TCMs and other traditional medicines ( Arulandhu et al, 2017 ; Arulandhu et al, 2019 ; Yao et al, 2022 ; Zhu et al, 2022 ). Chromatographic fingerprints are known to provide a high resolution for the detection of target compounds of known ingredients and are a well-applied, basic authentication tool for herbal remedies such as TCMs ( Liang et al, 2010 ; Booker et al, 2016 ; Raclariu et al, 2017 ; Fitzgerald et al, 2019 ; Heinrich et al, 2022 ). Furthermore, fingerprinting techniques can allow the consideration of the complexity of herbal products by evaluating the whole chemical profile and extracting a common pattern to be used as a criterion for elaborating the individual formulation ( Noviana et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Complementary authentication of Chinese herbal products to treat endometriosis using DNA metabarcoding and HPTLC shows a high level of variability

Mück,

Scotti,

Mauvisseau

et al. 2023

Front. Pharmacol.

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Traditional Chinese Medicine (TCM) is popular for the treatment of endometriosis, a complex gynecological disease that affects 10% of women globally. The growing market for TCMs has yielded a significant incentive for product adulteration, and although emerging technologies show promise to improve their quality control, many challenges remain. We tested the authenticity of two traditional Chinese herbal formulae used in women’s healthcare for the treatment of endometriosis, known as Gui Zhi Fu Ling Wan (FL) and Ge Xia Zhu Yu Tang (GX). Dual-locus DNA metabarcoding analysis coupled with high-performance thin-layer chromatography (HPTLC) were used to authenticate 19 FL and six GX commercial herbal products, as well as three ad hoc prepared artificial mixtures. HPTLC was able to detect most of the expected ingredients via comparative component analysis. DNA metabarcoding was able to detect an unexpected species diversity in the products, including 38 unexpected taxa. Chromatography has a resolution for all species indirectly through the identification of marker compounds for the different species ingredients. Metabarcoding on the other hand yields an overview of species diversity in each sample, but interpretation of the results can be challenging. Detected species might not be present in quantities that matter, and without validated quantification, some detected species can be hard to interpret. Comparative analysis of the two analytical approaches also reveals that DNA for species might be absent or too fragmented to amplify as the relevant chemical marker compounds can be detected but no amplicons are assigned to the same species. Our study emphasizes that integrating DNA metabarcoding with phytochemical analysis brings valuable data for the comprehensive authentication of Traditional Chinese Medicines ensuring their quality and safe use.

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Applied Barcoding: The Practicalities of DNA Testing for Herbals

Howard

Lockie-Williams

Slater

2020

Plants

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DNA barcoding is a widely accepted technique for the identification of plant materials, and its application to the authentication of commercial medicinal plants has attracted significant attention. The incorporation of DNA-based technologies into the quality testing protocols of international pharmacopoeias represents a step-change in status, requiring the establishment of standardized, reliable and reproducible methods. The process by which this can be achieved for any herbal medicine is described, using Hypericum perforatum L. (St John’s Wort) and potential adulterant Hypericum species as a case study. A range of practical issues are considered including quality control of DNA sequences from public repositories and the construction of individual curated databases, choice of DNA barcode region(s) and the identification of informative polymorphic nucleotide sequences. A decision tree informs the structure of the manuscript and provides a template to guide the development of future DNA barcode tests for herbals.

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What’s in the box? Authentication ofEchinaceaherbal products using DNA metabarcoding and HPTLC

Cited by 3 publications

References 33 publications

PhytoAuthent: Molecular authentication of complex herbal food supplements for safety and efficacy

PhytoAuthent: Molecular authentication of complex herbal food supplements for safety and efficacy

Complementary authentication of Chinese herbal products to treat endometriosis using DNA metabarcoding and HPTLC shows a high level of variability

Applied Barcoding: The Practicalities of DNA Testing for Herbals

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