What's in a name? Aschersonia insperata: a new pleoanamorphic fungus with characteristics of Aschersonia and Hirsutella

Abstract

A new anamorphic species from a Philippine tropical forest occurs as reddish-orange to orange, tuberculate stromata on unidentified homopteran larvae, and produces both Aschersonia and Hirsutella-like synanamorphs. A molecular phylogenetic analysis was conducted to determine the most appropriate generic placement for this fungus. Based on its phylogenetic relationships, a comparison of the complexity and persistence of each anamorph, and the speculated relevance of each synanamorph to survival, we describe the new fungus as Aschersonia insperata sp. nov.

A taxonomic revision of the genus Conidiobolus (Ancylistaceae, Entomophthorales): four clades including three new genera

“…The nucLSU region was amplified with the primers LR0R and LR5 (Vilgalys and Hester 1990), the nucSSU region with nucSSU-0021-5' (Gargas and DePriest 1996) and nucSSU-1780-3' (DePriest 1993) and EF-1α region with the primers EF983 and EF1aZ-1R (http://www.aftol.org/primers.php). These PCR reactions have been described by Liu et al (2005), Jensen et al (1998) and Nie et al (2012). The primers used for the mtSSU region were mtSSU1 and mtSSU2R and the PCR reaction was performed using the following cycling parameters: denaturation at 94 °C for 3 min, followed by 35 cycles of denaturation at 94 °C for 1 min, annealing at 52 °C for 1 min, extension at 72 °C for 1 min and finalised with an extra extension at 72 °C for 7 min (Zoller et al 1999).…”

Three new species of Conidiobolus sensu stricto from plant debris in eastern China

“…Universal primer pairs LR0R (5'-ACC CGC TGA ACT TAA GC-3') and LR5 (5'-TCC TGA GGG AAA CTT CG-3') (Vilgalys and Hester 1990), mtSSU1 (5'-GCW GCA GTG RGG AAT NTT GGR CAA T-3') and mtSSU2R (5'-GTR GAC TAM TSR GGT ATC TAA TC-3') (Zoller et al 1999) and EF983 (5'-GCY CCY GGH CAY CGT GAY TTY AT-3') and EF1aZ-1R (5'-ACA TCW CCG ACA CCC TTG ATC TTG -3') (Nie et al 2012) were used for the amplification of the partial region of nucLSU, mtSSU and TEF1, respectively. The PCR reactions followed those in Liu et al (2005) and Nie et al (2012Nie et al ( , 2020. A 50 μl mixture contained 200 μM dNTPs each, 1 × Mgfree buffer, 2.5 mM MgCl 2 , 0.5 μM primers each, 50 ng genomic DNA and 2 U Taq polymerase (Super Pfx DNA Polymerase, Cowinbioscience Co. Ltd., Shanghai, China).…”

Moelleriella zhongdongii: stroma development and identification of hirsutella-like and Aschersonia synanamorphs

“…Hirsutella-like anamorphs are rarely described in Moelleriella , apparently because of their fragile, ephemeral nature (Chaverri et al . 2008; Liu et al . 2005).…”

“…Hodge (Chaverri et al . 2008; Liu et al 2005). Although not well described, Petch (1921) observed a surface layer of scattered conidiophores and conidia on stromata of Moelleriella reineckiana Henn.…”