What new cell biology findings could bring to therapeutics: is it time for a phenome-project in Toxoplasma gondii?

Meissner, Markus; Klaus, Katrin

doi:10.1590/s0074-02762009000200010

Cited by 4 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years the adaptation of several reverse and forward genetic tools established T. gondii as an attractive model system for apicomplexan parasites and general molecular biology 142 . However, these techniques are not frequently employed for the study of host-pathogen interactions and most of the times, the only in vivo experiments performed to study the function of a gene of interest are virulence studies (survival experiments), without a detailed analysis of the immune response 143 , 144 .…”

Section: Genetic Tools and In Vivo Imaging To Study The Immunopathogementioning

confidence: 99%

Toxoplasma gondiiand the blood-brain barrier

2012

Self Cite

View full text Add to dashboard Cite

Infection with the protozoan parasite Toxoplasma gondii is characterized by asymptomatic latent infection in the central nervous system and skeletal muscle tissue in the majority of immunocompentent individuals. Life-threatening reactivation of the infection in immunocompromized patients originates from rupture of Toxoplasma cysts in the brain. While major progress has been made in our understanding of the immunopathogenesis of infection the mechanism(s) of neuroinvasion of the parasite remains poorly understood. The present review presents the current understanding of blood-brain barrier (patho)physiology and the interaction of Toxoplasma gondii with cells of the blood-brain barrier.

show abstract

Section: Genetic Tools and In Vivo Imaging To Study The Immunopathogementioning

confidence: 99%

Toxoplasma gondiiand the blood-brain barrier

2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Mouse Phenome Project was launched a decade ago to promote new phenotyping initiatives under standardized conditions and to collect the data in a central public database [Maddatu et al, 2012], and similar efforts are underway for the zebrafish [Cheng et al, 2011], the rat [Mashimo et al, 2005; Serikawa et al, 2009], and other organisms such as Toxoplasma gondii [Meissner and Klaus, 2009]. Driving all these projects is the conviction that a precise analysis of the phenotype is needed to take full advantage of findings from genetic and genomic in order to understand biology.…”

Section: Are We Ready For a Human Phenome Project?mentioning

confidence: 99%

Deep phenotyping for precision medicine

2012

View full text Add to dashboard Cite

ABSTRACT:In medical contexts, the word "phenotype" is used to refer to some deviation from normal morphology, physiology, or behavior. The analysis of phenotype plays a key role in clinical practice and medical research, and yet phenotypic descriptions in clinical notes and medical publications are often imprecise. Deep phenotyping can be defined as the precise and comprehensive analysis of phenotypic abnormalities in which the individual components of the phenotype are observed and described. The emerging field of precision medicine aims to provide the best available care for each patient based on stratification into disease subclasses with a common biological basis of disease. The comprehensive discovery of such subclasses, as well as the translation of this knowledge into clinical care, will depend critically upon computational resources to capture, store, and exchange phenotypic data, and upon sophisticated algorithms to integrate it with genomic variation, omics profiles, and other clinical information. This special issue of Human Mutation offers a number

show abstract

“…The gene encoding this antigen has 2122 bp (5,6). Rhoptry protein 1 is expressed in tachyzoite, bradyzoite and sporozoite (7).…”

Section: Introductionmentioning

confidence: 99%

A preliminary Study: Expression of Rhoptry Protein 1 (ROP1) Toxoplasma gondii in Prokaryote System

Eslamirad¹,

Ghaffarifar²,

Shojapour³

et al. 2013

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background:Toxoplasma gondii is an obligatory intracellular protozoan parasite, which infects human beings. Since the current antigens used for diagnosis or vaccination are contaminated with non parasitic material in which the parasite is grown, it is tried to produce recombinant antigens to design vaccines against toxoplasmosis, or make diagnostic kits. Choosing the type of antigen to produce recombinant vaccine or diagnostic kits is considerably important. The rhoptry protein 1 is one of the excretory-secretary antigens of Toxoplasma which seems to be an appropriate candidate in production of recombinant vaccines and diagnostic kits. Objectives: The current study aimed to produce rhoptry protein 1 from Iranian strains of Toxoplasma for further investigations. Materials and Methods: Genomic DNA was isolated from tachyzoite of parasite by phenol chloroform method and gene fragment was amplified by polymerase chain reaction. The polymerase chain reaction products were ligated into restriction enzymes sites of pTZ57R/T cloning vector. The product was sub-cloned into a prokaryotic expression plasmid (pET32a). The recombinant expression vector containing rhoptry protein 1 sequence was transformed into Escherichia coli BL21 pLysS and was induced by isopropyl β-D-1-thiogalactopyranoside. The sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting methods were used to confirm the production of protein. Results:The result showed that the desired molecular weight protein is produced. Recombinant protein was confirmed by western blot using Ni-NTA-conjugate. Conclusions:The result of this study showed that recombinant ROP1 Toxoplasma was produced successfully. In the present study, unlike other studies, the goal was to express full length of ROP1. Also this protein was produced alone not fused with other proteins.

show abstract

What new cell biology findings could bring to therapeutics: is it time for a phenome-project in Toxoplasma gondii?

Cited by 4 publications

References 30 publications

Toxoplasma gondiiand the blood-brain barrier

Toxoplasma gondiiand the blood-brain barrier

Deep phenotyping for precision medicine

A preliminary Study: Expression of Rhoptry Protein 1 (ROP1) Toxoplasma gondii in Prokaryote System

Contact Info

Product

Resources

About