As more libraries have made a persistent effort to build Makerspace expecting to proceed to innovate, there have been a growing number of studies accessing this practice. However, few studies have explored gender orientation and preferences produced when Makerspace is used. Through the questionnaire, this study investigates the service condition of Makerspace. The results indicate that the gender colonies' differences and preference exist: male students are more addicted to the 3D service; female students feel more interested in drone service to a huge extent; digital modeling is most welcome by the colony. This provides valuable information for librarians how they could promote the service quality and make their future plan. In addition, implications and recommendations are also addressed.