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M, Vergine; Frati, Maurizio; Pozzo, Sergio; Bertazzolo, Walter

doi:10.1111/j.1748-5827.2006.00023.x

Cited by 1 publication

(1 citation statement)

References 2 publications

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“…Finally, the sample was stored at -80°C for later analysis. The methods of the skin lesion morbidity scoring system were from a previous study (40). For the determination of physical and immune barrier-related parameters, 10% (w/v) of skin tissue homogenates were prepared with saline (4°C) and centrifuged (6,000 g, 20 min).…”

Section: Sampling and Biochemical Parameter Analysismentioning

confidence: 99%

Mannan Oligosaccharides Application: Multipath Restriction From Aeromonas hydrophila Infection in the Skin Barrier of Grass Carp (Ctenopharyngodon idella)

et al. 2021

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The objective of this study was to evaluate the efficacy of dietary Mannan oligosaccharides (MOS) supplementation on skin barrier function and the mechanism of on-growing grass carp (Ctenopharyngodon idella). Five hundred forty grass carp were fed for 60 days from the growing stage with six different levels of MOS diets (0, 200, 400, 600, 800, and 1,000 mg kg-1). At the end of the growth trial, the 14-day Aeromonas hydrophila challenge experiment has proceeded. The obtained data indicate that MOS could (1) decline skin lesion morbidity after being challenged by the pathogenic bacteria; (2) maintain physical barrier function via improving antioxidant ability, inhibiting excessive apoptosis, and strengthening the tight junction between the epithelial cell and the related signaling pathway (Nrf2/Keap1, p38MAPK, and MLCK); and (3) regulate immune barrier function by modulating the production of antimicrobial compound and expression of involved cytokines and the related signaling pathway (TOR and NFκB). Finally, we concluded that MOS supplementation reinforced the disease resistance and protected the fish skin barrier function from Aeromonas hydrophila infection.

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Section: Sampling and Biochemical Parameter Analysismentioning

confidence: 99%