What is the significance of CD34 immunostaining in the extraglomerular and intraglomerular mesangium?

Gluhovschi, Cristina; Gluhovschi, Gheorghe; Potencz, Elena; Herman, Diana; Petrică, Ligia; Velciov, Silvia; Bozdog, Gheorghe; Bob, Flaviu; Vernic, Corina; Cioca, Daniel

doi:10.1007/s00428-008-0647-0

Cited by 9 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8A and 8B) [64]. Alternatively, Gluhovschi et al indicated CD34 does not significantly correlate with mesangial α-SMA [19]. Swimming exercise at 60 min program restored its level to normal (Fig.…”

Section: Discussionmentioning

confidence: 90%

“…At the level of extraglomerular or intraglomerular mesangium, CD34 may signal either the presence of HSCs, or conversely, may be a marker of transdifferentiation [19]. Immunohistochemical co-staining of CD34 and α-SMA has been used to study various glomerulonephritis (GN) as a transformed mesangial cell marker [64].…”

Section: Discussionmentioning

confidence: 99%

“…CD34, a sialomucin-type glycophosphoprotein acting as an adhesion molecule, is a marker of haematopoietic stem cells (HSCs) and leukemic cells [16], [17], [18], [19]. CD34 is concentrated mostly in mesangial area and endothelial surfaces in glomeruli [18].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Swimming Exercise Prevents Fibrogenesis in Chronic Kidney Disease by Inhibiting the Myofibroblast Transdifferentiation

et al. 2012

View full text Add to dashboard Cite

Background The renal function of chronic kidney disease (CKD) patients may be improved by a number of rehabilitative mechanisms. Swimming exercise training was supposed to be beneficial to its recovery. Methodology/Principal Findings Doxorubicin-induced CKD (DRCKD) rat model was performed. Swimming training was programmed three days per week, 30 or 60 min per day for a total period of 11 weeks. Serum biochemical and pathological parameters were examined. In DRCKD, hyperlipidemia was observed. Active mesangial cell activation was evidenced by overexpression of PDGFR, P-PDGFR, MMP-2, MMP-9, α-SMA, and CD34 with a huge amount collagen deposition. Apparent myofibroblast transdifferentiation implicating fibrogenesis in the glomerular mesangium, glomerulonephritis and glomeruloscelorosis was observed with highly elevated proteinuria and urinary BUN excretion. The 60-min swimming exercise but not the 30 min equivalent rescued most of the symptoms. To quantify the effectiveness of exercise training, a physical parameter, i.e. “the strenuosity coefficient” or “the myokine releasing coefficient”, was estimated to be 7.154×10 −3 pg/mL-J. Conclusions The 60-min swimming exercise may ameliorate DRCKD by inhibiting the transdifferentiation of myofibroblasts in the glomerular mesangium. Moreover, rehabilitative exercise training to rescue CKD is a personalized remedy. Benefits depend on the duration and strength of exercise, and more importantly, on the individual physiological condition.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Swimming Exercise Prevents Fibrogenesis in Chronic Kidney Disease by Inhibiting the Myofibroblast Transdifferentiation

et al. 2012

View full text Add to dashboard Cite

show abstract

“…To better quantify the histological lesions, a scoring system adapted from Neumann [15] was employed on kidney biopsies. We used this scoring system in two previous reports [24,25].…”

Section: Discussionmentioning

confidence: 99%

The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity.

Gluhovschi

Gluhovschi²,

Potencz³

et al. 2010

Folia Histochem Cytobiol.

Self Cite

View full text Add to dashboard Cite

Endothelial cells (ECs) are active participants of an inflammatory process in glomeruli. EC damage has been shown to play an important role in the progression of glomerulonephritis (GN). The degree of glomerular and peritubular capillary loss in models of progressive renal disease correlates with the severity of glomerulosclerosis and interstitial fibrosis. The aim of our study was to analyze the association of vWF, CD31 and CD34 immunoreactivity with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity in GN. A cross-sectional study of 22 patients with GN was conducted. Conventional stains (hematoxylin-eosin, periodic acid Schiff and Trichrome Gömöri stains) and immunohistochemistry (vWF, CD31 and CD34) were employed on kidney biopsies. Activity and chronicity of GN, as well as glomerular segmental sclerosis and interstitial fibrosis, were evaluated according to a scoring system initially used for lupus nephritis and antineutrophil-cytoplasmic-antibody-associated vasculitis. Immunohistochemistry was assessed using a semi-quantitative score. Statistical analysis was performed using EpiInfo 6.04. The mean patient age was 46.68±14.09; 14 patients were male, and eight were female. Performing Spearman`s rank correlation test, no correlation was found between each marker and glomerular segmental sclerosis, interstitial fibrosis, activity and chronicity, which suggests a loss of these markers and microvasculature involvement.

show abstract

“…In one of our previous studies we have analyzed the presence of CD34 at the level of the extraglomerular mesangium, concluding that it could be either a marker of stem cells entering the glomerulus at this level or a marker of mesangial activation [9].…”

Section: Cd34-positive Fibroblast-like Cells In Glomerulonephritismentioning

confidence: 99%

CD34+ fibroblast-like cells in the interstitial infiltrates in glomerulonephritis – an immunohistochemical observation

Gluhovschi¹,

Potencz²,

Lazăr³

et al. 2012

pjp

Self Cite

View full text Add to dashboard Cite

CD34 cells in the interstitial infiltrates in glomerulonephritis (GN) could be the turning point between regenerative processes and interstitial fibrosis. The aim of our study was to assess the presence of CD34+ cells in the interstitial infiltrates in GN. A cross-sectional study of 33 patients with glomerulonephritis, mean age: 43.3 ±11.31 years, 20 male and 13 female, was conducted. Conventional stains, as well as immunohistochemistry for the CD34 antigen were employed on kidney biopsies. Strength of immunohistochemical reaction was assessed semi-quantitatively. Regarding the percentage of cases with CD34+ cells in the interstitial infiltrates out of 33 patients: cells of interstitial infiltrates were 27.3% positive. The percentage of cases showing CD34+ cells at the level of interstitial infiltrates was: 44.4% in FSGS, 14.3% in membranoproliferative GN, 28.6% in membranous nephropathy, 20% in mesangial proliferative GN, 0% in minimal change disease, and 50% in crescentic GN. With the exception of minimal change disease, CD34+ cells were found in the interstitial infiltrates in all histopathological forms of GN. Some of these cells were spindle-shaped fibroblast-like cells. As inflammation in the tubulointerstitial compartment either resolves or proceeds to fibrosis, aims at reversing this process will benefit from analyses of the interstitial infiltrates harboring CD34+ cells.

show abstract

What is the significance of CD34 immunostaining in the extraglomerular and intraglomerular mesangium?

Cited by 9 publications

References 35 publications

Swimming Exercise Prevents Fibrogenesis in Chronic Kidney Disease by Inhibiting the Myofibroblast Transdifferentiation

Swimming Exercise Prevents Fibrogenesis in Chronic Kidney Disease by Inhibiting the Myofibroblast Transdifferentiation

The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity.

CD34+ fibroblast-like cells in the interstitial infiltrates in glomerulonephritis – an immunohistochemical observation

Contact Info

Product

Resources

About