What is the most reliable solid culture medium for tuberculosis treatment trials?

Joloba, Moses; Johnson, John L.; Feng, Pei Jean I; Bozeman, Lorna; Goldberg, Stefan; Morgan, Kàren; Gitta, Phineas; Boom, Henry W.; Heilig, Charles M.; Mayanja‐Kizza, Harriet; Eisenach, Kathleen D.

doi:10.1016/j.tube.2014.03.002

Cited by 22 publications

(21 citation statements)

References 10 publications

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“…Our comparison included commercially prepared LJ with and without antibiotics. Given the differences in LJ media formulations and sterilization techniques used in the preparation of LJ, performance characteristics for locally prepared media [18] , specifically LJ containing antibiotics, may differ from what we have reported here. Patient samples used for our evaluation were collected prior to initiation of anti-TB therapy, and at a single time point.…”

Section: Discussioncontrasting

confidence: 67%

“…In the few facilities where culture is routinely performed, maintaining acceptable contamination rates remains a challenge and could potentially diminish the utility of culture in this setting. Antibiotic-containing solid media could theoretically reduce contamination rates; however studies describing the potential impact on the yield of MTBC [18,19] , specifically on LJ solid media [20] are limited.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Mycobacterium tuberculosis complex Yield and Contamination Rates using Lowenstein-Jensen with and without Antibiotics in Western Kenya

Okumu¹

2017

JMSCR

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Section: Discussioncontrasting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Comparison of Mycobacterium tuberculosis complex Yield and Contamination Rates using Lowenstein-Jensen with and without Antibiotics in Western Kenya

Okumu¹

2017

JMSCR

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“…However, some researchers have suggested that the supplementation with casein pancreatic digest makes no difference to the growth of M. tuberculosis. Moreover, studies evaluating different media for M. tuberculosis detection in clinical samples have produced contradictory results regarding whether 7H10 or 7H11 agar medium is more reliable (26,27). Hence, there is no consensus among laboratories for preference between these two agar media for growth of M. tuberculosis, with use largely dictated by historical preference.…”

Section: Discussionmentioning

confidence: 99%

A Multilaboratory, Multicountry Study To Determine Bedaquiline MIC Quality Control Ranges for Phenotypic Drug Susceptibility Testing

et al. 2016

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jThe aim of this study was to establish standardized drug susceptibility testing (DST) methodologies and reference MIC quality control (QC) ranges for bedaquiline, a diarylquinoline antimycobacterial, used in the treatment of adults with multidrug-resistant tuberculosis. Two tier-2 QC reproducibility studies of bedaquiline DST were conducted in eight laboratories using Clinical Laboratory and Standards Institute (CLSI) guidelines. Agar dilution and broth microdilution methods were evaluated. Mycobacterium tuberculosis H37Rv was used as the QC reference strain. Bedaquiline MIC frequency, mode, and geometric mean were calculated. When resulting data occurred outside predefined CLSI criteria, the entire laboratory data set was excluded. N ewer drugs are being developed to counter the growing problem of multidrug-resistant (MDR) strains of Mycobacterium tuberculosis. Of the 9.6 million estimated new M. tuberculosis cases occurring globally in 2014, 3.3% were MDR, in addition to 20% of previously treated tuberculosis (TB) cases estimated to have MDR; this equates to an overall estimate of 480,000 people with MDR-TB annually. Furthermore, current treatment outcome data for patients started on MDR-TB treatment in 2015 suggest a success rate of only 50% (1). The approval of new antimycobacterials effective against MDR-TB strains has highlighted the need for validated and standardized drug susceptibility testing (DST) methods to enhance patient care and for facilitating drug resistance surveillance.Bedaquiline, a diarylquinoline antimycobacterial (2), has received accelerated/conditional approval for use based on phase II trials in the United States (2012), the European Union (2014), and 7 countries with high MDR-TB burden (3-8). Interim policy guidance for the use of bedaquiline as part of combination therapy for adults who have pulmonary MDR-TB has been issued by the World Health Organization (WHO) and the U.S. Centers for Disease Control and Prevention (CDC) (9, 10).Preliminary DST methodology for bedaquiline was previously piloted and then used in two phase II clinical studies (3-6). In these studies, bedaquiline DST was performed by 7H11 agar dilution and 7H9 broth microdilution methods using the resazurin microtiter assay (REMA) (11,12). For bedaquiline DST, the standard quality control (QC) strain M. tuberculosis H37Rv should be used under the same conditions as the clinical M. tuberculosis isolates to ensure that the bedaquiline MIC of the reference falls within a predefined QC range. For QC purposes when testing clinical isolates prior to the present study, the provisional bedaquiline MIC ranges against strain H37Rv were 0.03 to 0.12 g/ml for 7H11 agar and 0.03 to 0.12 g/ml for REMA (7H9

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“…Таким образом, данная среда хорошо подходит для культивирования M. tuberculosis в условиях in vitro и позволяет получать данные по чувствительности к антибиотикам, сопоставимые с результатами, полученными на среде Левенштейна-Йенсена [8][9][10]. Однако в ряде работ показано, что культуральные свойства микобактерий зависят от состава питательной среды [11,12]. В частности, после инокуляции микобактерий на чашку со средой Миддлбрук 7H11 видимые колонии появляются значительно раньше, чем на чашке со средой Левенштейна-Йенсена [13].…”

Section: Introductionunclassified

Influence of cultivation conditions on the proteomic profile of Mycobacterium tuberculosis H37RV

et al. 2017

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Comparative proteomic profiling of M. tuberculosis H37Rv strains cultured on two different nutrient media, Levenstein-Jensen and Middlebrook 7H11, was performed using a label-free LC-MS/MS approach. It was shown that results obtained from two media possessed high convergence. The only difference was observed in the representation of fumarate reductase FrdB, its abundance was higher in the mycobacterial cells cultured on Levenstein-Jensen medium. The correlation analysis of biological repeats revealed the high convergence of the results obtained from Middlebrook 7H11 medium. Thus, we can conclude that the use of the Middlebrook 7H11 medium is most appropriate in the scientific laboratory.

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What is the most reliable solid culture medium for tuberculosis treatment trials?

Cited by 22 publications

References 10 publications

Comparison of Mycobacterium tuberculosis complex Yield and Contamination Rates using Lowenstein-Jensen with and without Antibiotics in Western Kenya

Comparison of Mycobacterium tuberculosis complex Yield and Contamination Rates using Lowenstein-Jensen with and without Antibiotics in Western Kenya

A Multilaboratory, Multicountry Study To Determine Bedaquiline MIC Quality Control Ranges for Phenotypic Drug Susceptibility Testing

Influence of cultivation conditions on the proteomic profile of Mycobacterium tuberculosis H37RV

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