What Is the Best Technique for the Detection of Red Cell Alloantibodies?

Engelfriet, C. P.; Reesink, H. W.; Barbolla, L; Carbonell, F; Mitchell, R.; Contreras, M.; Knight, R.; Goldman, Michel; Décary, Francine; Garratty, George; Gerber, H.; Hustinx, Hein; Kornstad, L.; Moulds, John J.; Overbeeke, M. A. M.; Pirkola, Anna; Voak, D.; Ouwehand, Willem H.

doi:10.1159/000462857

Cited by 4 publications

(2 citation statements)

References 1 publication

(7 reference statements)

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“…It is important to emphasize that no single technique will detect all clinically significant antibodies (9,11,12). Interestingly, regardless of technique, survey participants had difficulties in identifying combined Rh antibodies, for instance anti-c with anti-E, because anti-c often masks the presence of anti-E. Anti-c with anti-K also proved problematic, with anti-c masking antibodies anti-K.…”

Section: Discussionmentioning

confidence: 99%

Twelve years of the Brazilian External Quality Assessment Program in Immunohematology: benefits of the program

Melo

Pellegrino

Bianco³

et al. 2005

J. Clin. Lab. Anal.

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The Brazilian External Quality Assessment Program in Immunohematology (BEQAPI) was introduced with the objective of evaluating the quality of diagnosis in immunohematology. From 1992 to 2003, proficiency tests for ABO grouping, Rh (D, C, c, E, e), K phenotyping, direct antiglobulin testing (DAT), antibody screening (AS), and antibody identification (AI) were performed. A total of 41 evaluations were carried out in 223 institutions. Over the period of 12 years, the program included 8,014 ABO typing, 8,000 RhD typing, 5,193 Rh typing (C, c, E, e), 5,101 K phenotyping, 7,939 AS, 4,533 AI, and 7,912 DATs. Erroneous responses were classified as clerical, technical, or undetermined. A substantial proportion of erroneous responses due to clerical errors occurred in ABO typing (76/76 errors), RhD typing (34/58 errors), and Rh phenotyping (50/73 errors). Technical errors occurred predominantly for weak D (91/95 errors), AS (252/301 errors), and AI (321/335 errors). Based on these results, since 1996, participants have received "Questions and Case Studies" in Immunohematology as an incentive for training and education. The results of the present study show an improvement in the performance of participants in the course of the program. We found that a well-organized external proficiency program can contribute to the improvement of quality of testing in Immunohematology.

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Section: Discussionmentioning

confidence: 99%

Twelve years of the Brazilian External Quality Assessment Program in Immunohematology: benefits of the program

Melo

Pellegrino

Bianco³

et al. 2005

J. Clin. Lab. Anal.

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“…. tive for antigen detection, alloantibody screening/identification and cross-matching [2][3][4]. The gel test has also appeared to be useful in patients with auto-immune haemolytic anaemia (AIHA), mainly because it detects auto-antibody in so-called 'Coombs-negative' haemolytic anaemia [5].…”

Section: Abstract Background and Objectivesmentioning

confidence: 99%

Gel Test Application for IgG Subclass Detection in Auto-Immune Haemolytic Anaemia

1997

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Background and objectives: Commercially available gel test microtubes are not available with antisera for the determination of IgG subclasses. The aim of this study was to adapt the gel technique for this purpose and apply it to the investigation of patients with autoimmune haemolytic anaemia (AIHA). Materials and methods: We studied 66 red cell samples from 49 patients with AIHA of the warm-active IgG type. Standard serologic and haematologic methods were used. We adapted the DiaMed gel test by using IgG-subclass antisera. Results: We found the adapted test useful in determining the subclass of autoantibodies in eluates. We could identify the IgG subclass in all the AIHA patients, even those that were ‘Coombs-negative’, with less than 200 IgG molecules bound in vivo per red cell. Comparison of the gel test with the standard spin tube test and the microtiter plate test showed the superiority of the gel test, i.e., detection of IgG subclasses was much better than with the tube test and the results were more clearcut than those of the microplate test. The gel test is also the simplest and least time-consuming and permits a later reading of results. Application of the test in our 66 samples confirmed that IgGl was the most frequent (96%). In 59% of the cases it was accompanied by IgG of other subclasses. Multiple subclasses were most common in the cases with stronger in vivo IgG red cell sensitization and severe haemolysis. Accompanying IgG3 was detected only in patients with obvious haemolysis. Conclusion: The gel test is more sensitive than other procedures for the determination of IgG subclass and has the advantages of simplicity, rapidity, low cost, and stability of the agglutinates.

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Application of noninvasive phagocytic cellular assays using autologous monocytes to assess red cell alloantibodies in sickle cell patients

Fabron

Baleotti

Mello

et al. 2004

Transfusion and Apheresis Science

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What Is the Best Technique for the Detection of Red Cell Alloantibodies?

Cited by 4 publications

References 1 publication

Twelve years of the Brazilian External Quality Assessment Program in Immunohematology: benefits of the program

Twelve years of the Brazilian External Quality Assessment Program in Immunohematology: benefits of the program

Gel Test Application for IgG Subclass Detection in Auto-Immune Haemolytic Anaemia

Application of noninvasive phagocytic cellular assays using autologous monocytes to assess red cell alloantibodies in sickle cell patients

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