Background This study was to explore the knowledge, attitudes, and behaviors (KAB) regarding brushing teeth with powered toothbrush among doctors. Methods This population-based, cross-sectional study was conducted by using a self-administered questionnaire regarding powered toothbrush based on the KAB model. Multistage cluster sampling was used to enrol doctors. Results A total of 403 doctors were included in the study. The proportion of “need to be strengthen” on knowledge, attitude and behaviors was 68.0 percent, 71.0 percent and 80.4 percent, respectively. The usage rate of powered toothbrush was low in doctors (12.2 percent). Doctors from dental department had higher knowledge (p<0.001) and attitude (p<0.01) scores than the doctors from non-dental department. Doctors from the district with a higher gross regional product had higher knowledge (p<0.01) and behaviors score (p<0.001), and doctors from higher-level hospitals had higher knowledge (p<0.001). Using powered toothpaste was positively associated with the knowledge (p<0.001), attitude (p<0.05) and behaviors (p<0.001) scores. Conclusions The majority of doctors’ knowledge, attitude and behaviors regarding powered toothbrush were needed to be strengthened. Specific health education is needed to improve the knowledge, attitude and behaviors regarding powered toothbrush.