What is metabolomics all about?

Roessner, Ute; Bowne, Jairus

doi:10.2144/000113133

Cited by 163 publications

(120 citation statements)

References 14 publications

Supporting

Mentioning

105

Contrasting

Unclassified

Order By: Relevance

“…A principle of metabolomics is that many human diseases are caused or result from an abnormal metabolic state (25). Metabolites represent a reading of the functional state of the physiological environment, and metabolomics is a unique tool of discovery because it identifies multiple endogenous markers from a single sample without preconception or selection bias (31). Initial differences in metabolites may be predictive of disease severity or clinical outcome, and changes over time may be useful in characterizing therapeutic response.…”

mentioning

confidence: 99%

Metabolic consequences of sepsis-induced acute lung injury revealed by plasma ¹H-nuclear magnetic resonance quantitative metabolomics and computational analysis

Stringer

Serkova

Karnovsky

et al. 2011

American Journal of Physiology-Lung Cellular and Molecular Phys

141

136

View full text Add to dashboard Cite

Metabolomics is an emerging component of systems biology that may be a viable strategy for the identification and validation of physiologically relevant biomarkers. Nuclear magnetic resonance (NMR) spectroscopy allows for establishing quantitative data sets for multiple endogenous metabolites without preconception. Sepsis-induced acute lung injury (ALI) is a complex and serious illness associated with high morbidity and mortality for which there is presently no effective pharmacotherapy. The goal of this study was to apply ¹H-NMR based quantitative metabolomics with subsequent computational analysis to begin working towards elucidating the plasma metabolic changes associated with sepsis-induced ALI. To this end, this pilot study generated quantitative data sets that revealed differences between patients with ALI and healthy subjects in the level of the following metabolites: total glutathione, adenosine, phosphatidylserine, and sphingomyelin. Moreover, myoinositol levels were associated with acute physiology scores (APS) (ρ = -0.53, P = 0.05, q = 0.25) and ventilator-free days (ρ = -0.73, P = 0.005, q = 0.01). There was also an association between total glutathione and APS (ρ = 0.56, P = 0.04, q = 0.25). Computational network analysis revealed a distinct metabolic pathway for each metabolite. In summary, this pilot study demonstrated the feasibility of plasma ¹H-NMR quantitative metabolomics because it yielded a physiologically relevant metabolite data set that distinguished sepsis-induced ALI from health. In addition, it justifies the continued study of this approach to determine whether sepsis-induced ALI has a distinct metabolic phenotype and whether there are predictive biomarkers of severity and outcome in these patients.

show abstract

mentioning

confidence: 99%

Metabolic consequences of sepsis-induced acute lung injury revealed by plasma ¹H-nuclear magnetic resonance quantitative metabolomics and computational analysis

Stringer

Serkova

Karnovsky

et al. 2011

American Journal of Physiology-Lung Cellular and Molecular Phys

141

136

View full text Add to dashboard Cite

show abstract

“…10 All omics fields measure the complete complement of biological features, and metabolomics specifically measures intracellular and extracellular metabolites in an unbiased and quantitative manner. 11 This of course is impossible due to the large range of concentrations, polarities, and stabilities of metabolites present in living cells.…”

Section: Metabolomics and Metabolite Profilingmentioning

confidence: 99%

The Uses and Future Prospects of Metabolomics and Targeted Metabolite Profiling in Cell Factory Development

Harrison

2013

Industrial Biotechnology

View full text Add to dashboard Cite

The development of cell factories for the production of chemicals has traditionally relied on measurements of product metabolite titers to assess the performance of genetically manipulated strains. With the development of improved metabolomics and targeted metabolite profiling methods, these broader measurements of the cellular metabolic state are now becoming part of the toolbox used to characterize cell factories. In this review we briefly summarize the benefits and challenges of global metabolomics and targeted metabolite profiling methods and discuss the application of these methods in both pathway discovery and cell factory engineering. We focus particularly on exploring the potential of global metabolomics to complement more traditional targeted methods. We conclude the review by discussing emerging trends in metabolomics and how these developments can aid the engineering of better cell factories in the future.

show abstract

“…In fact, metabolomics not only defines the relationship between genotype and its resulting phenotype, but also clarifies the influence that stressful environmental conditions have on metabolism itself (Roessner and Bowne, 2009). The quantitative measurement of metabolites could thus reflect the status of a cellular system and its interaction with the surrounding environment.…”

Section: General Principles Of Metabolomicsmentioning

confidence: 99%

“…The metabolomic approach has been utilized to investigate the physiological status of cells, tissues and organisms, to diagnose diseases, measure response to treatments, or to identify perturbed pathways due to disease or treatment (Roessner and Bowne, 2009;Nordstrom and Lewensohn, 2010). However, the most valuable and promising clinical application of metabolomics is the discovery of biomarkers, i.e.…”

Section: General Principles Of Metabolomicsmentioning

confidence: 99%

Metabolomics in Reproductive Medicine: General Principles and Applications to the Study of Gametes, Embryos and Follicular Fluid

Piane

Molinari

Salvagno

et al. 2011

Journal of Reproductive and Stem Cell Biotechnology

View full text Add to dashboard Cite

After more than 30 years following the birth of Louise Brown, the field of assisted reproduction techniques (ART) is still burdened by low pregnancy rates and relatively high multiple pregnancy rates. Embryo selection is still based on morphological criteria and any new technologies that would increase the ability to identify the most viable gametes and embryos would certainly improve ART outcome. Metabolomic profiling of both gametes and embryos appears to be highly promising to achieve this goal. The review will focus on the several applications of metabolomics in ART, where this quantitative, non-invasive, rapid approach could result in the generation of metabolite markers with high clinical impact. Nevertheless, extensive validation studies are needed before embryo screening tests based on metabolomic profiling become available for clinical use.No author has any potential conflicts of interest of any nature.

show abstract

What is metabolomics all about?

Cited by 163 publications

References 14 publications

Metabolic consequences of sepsis-induced acute lung injury revealed by plasma ¹H-nuclear magnetic resonance quantitative metabolomics and computational analysis

Metabolic consequences of sepsis-induced acute lung injury revealed by plasma ¹H-nuclear magnetic resonance quantitative metabolomics and computational analysis

The Uses and Future Prospects of Metabolomics and Targeted Metabolite Profiling in Cell Factory Development

Metabolomics in Reproductive Medicine: General Principles and Applications to the Study of Gametes, Embryos and Follicular Fluid

Contact Info

Product

Resources

About

What is metabolomics all about?

Cited by 163 publications

References 14 publications

Metabolic consequences of sepsis-induced acute lung injury revealed by plasma 1H-nuclear magnetic resonance quantitative metabolomics and computational analysis

Metabolic consequences of sepsis-induced acute lung injury revealed by plasma 1H-nuclear magnetic resonance quantitative metabolomics and computational analysis

The Uses and Future Prospects of Metabolomics and Targeted Metabolite Profiling in Cell Factory Development

Metabolomics in Reproductive Medicine: General Principles and Applications to the Study of Gametes, Embryos and Follicular Fluid

Contact Info

Product

Resources

About

Metabolic consequences of sepsis-induced acute lung injury revealed by plasma ¹H-nuclear magnetic resonance quantitative metabolomics and computational analysis

Metabolic consequences of sepsis-induced acute lung injury revealed by plasma ¹H-nuclear magnetic resonance quantitative metabolomics and computational analysis