What is inside the jar? Forensically informative nucleotide sequencing (FINS) of a short mitochondrial COI gene fragment reveals a high percentage of mislabeling in jellyfish food products

Armani, Andrea; Tinacci, Lara; Castigliego, Lorenzo; Gianfaldoni, Daniela; Гуиди, Алессандра

doi:10.1016/j.foodres.2013.10.003

Cited by 58 publications

(65 citation statements)

References 24 publications

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“…Thermal treatments, ingredients and storage conditions are among the most important factors that can induce DNA degradation (Armani et al, 2013;Rodriguez-Ezpeleta et al, 2013). In fact, even though the cooking procedure used in this study was not comparable to that caused by canning processes, the amplificability was strongly affected.…”

Section: Factors Affecting Pcr Amplification When Using Full (Fdb) Anmentioning

confidence: 99%

“…Moreover, considering that a single commercial name can be used at the international level for different species, unscrupulous traders could take profit from this confusion by selling illegal products. Recent surveys showed that frauds are becoming widespread and seafood mislabelling has reached alarming levels (Armani et al, 2013;Carvalho, Neto, Brasil, & Oliveira, 2011;Wong & Hanner, 2008).…”

Section: Introductionmentioning

confidence: 99%

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DNA and Mini-DNA barcoding for the identification of Porgies species (family Sparidae) of commercial interest on the international market

et al. 2015

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a b s t r a c tThe morphological similarity among Sparidae species, which are characterized by a different market price, represents a serious problem for their trade and for stock management, since it encourages frauds for substitution. The most accredited morphological method for their identification is based on the dental-plate, but this approach is not simple and cannot be used for prepared products. When molecular methods are used the DNA degradation induced by cooking is the main drawback. In this work, we collected 314 reference tissues belonging to 75 Sparidae species and we produced a dataset of full (FDB) and mini-barcode (MDB) reference sequences starting from DNA extracted from fresh and ethanolpreserved tissues using universal primes. Moreover, some fresh samples were cooked. The FDB was successfully amplified in 91% (fresh), 50% (cooked) and 81% (ethanol-preserved) samples, while the amplification rates of the MDB were considerably higher in case of cooked (100%) and ethanol-preserved (94%) samples. The same primers were used for the amplification of the DNA obtained from 58 market samples (MS). All the DNA barcodes were compared with BOLD and GenBank using IDs and BLAST analysis. FDB was able to provide unambiguous species-level identifications for 53 (78%) and 44 (64.7%) reference samples analyzed on BOLD and GenBank, respectively. The Mini-DNA barcode (MDB) showed a lower discriminating power with 32 (45.7%) and 29 (41.4%) sequences unambiguously matched to a species on BOLD and GenBank. However, the MDB allowed to identify all the reference sequences as belonging to the Sparidae family. FDB and MDB showed a similar performance in analyzing the MS, allowing to highlight 21 (38%) mislabeled MS. Our study, while confirming the FDB as a reliable tool for fish authentication, proposes the MDB as a promising tool to recover molecular information in case of cooked products.

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Section: Factors Affecting Pcr Amplification When Using Full (Fdb) Anmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

DNA and Mini-DNA barcoding for the identification of Porgies species (family Sparidae) of commercial interest on the international market

et al. 2015

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“…The cost for these products are on an average two or three times higher than the popular ones (author's note). Considering that the use of valuable fish species directly affects the cost of the products, it is plausible that the practice of misleading labeling, widely reported for products intended for human consumption (Armani et al, 2013;Di Pinto et al, 2013;Pepe et al, 2007), could be also applied in the pet food sector to obtain a greater market appeal and an economic gain.…”

Section: Introductionmentioning

confidence: 99%

“…Such limitations emphasize the need of physical checks (Regulation (EC) 882/20024) that, in case of seafood, often rely on the utilization of analytical methods capable to provide species identification . The DNA-based techniques are routinely applied for the identification of processed fish products and feedstuffs, and shows greater efficiency than protein-based techniques in heat processed products (Ardura et al, 2012;Armani et al, 2013;Pepe et al, 2007). Among the several PCR-based methods, sequencing, namely Forensically Informative Nucleotide Sequencing (FINS) and DNA Barcoding, is the most frequently applied to fish and seafood species identification (Hellberg & Morrissey, 2011).…”

Section: Introductionmentioning

confidence: 99%

Fish species identification in canned pet food by BLAST and Forensically Informative Nucleotide Sequencing (FINS) analysis of short fragments of the mitochondrial 16s ribosomal RNA gene (16S rRNA)

et al. 2015

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“…Large quantities of effluent are generated as a byproduct of jellyfish processing, and need to be dealt with in a responsible way. Edible jellyfish may contain concerning amounts of aluminum (Wong et al 2010;Ogimoto et al 2012;Armani et al 2013;Zhang et al 2016), the consumption of which is linked to a number of negative health effects, including Alzheimer's disease (Perl and Brody 1980;Nayak 2002). The development of new processing technologies that either reduce the aluminum content in the edible products (e.g., Chen et al 2016) or eliminate the use of alum altogether is desirable (Hsieh and Rudloe 1994).…”

Section: Conclusion and Recommendationsmentioning

confidence: 99%

Jellyfish fisheries in the Americas: origin, state of the art, and perspectives on new fishing grounds

Brotz

Schiariti

López‐Martínez³

et al. 2016

Rev Fish Biol Fisheries

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Jellyfish (primarily scyphomedusae) fisheries have a long history in Asia, where jellyfish have been caught and processed as food for centuries. More recently, jellyfish fisheries have expanded to the Western Hemisphere, often driven by demand from Asian buyers and collapses of more traditional local fish stocks. Jellyfish fisheries have been attempted in numerous countries in North, Central, and South America, with varying degrees of success. Here, we chronicle the arrival of jellyfish fisheries in the Americas and summarize relevant information on jellyfish fishing, processing, and management. Processing technology for edible jellyfish has not advanced, and presents major concerns for environmental and human health. The development of alternative processing technologies would help to eliminate these concerns and may open up new opportunities for markets and species. We also examine the biodiversity of jellyfish species that are targeted for fisheries in the Americas. Establishment of new jellyfish fisheries appears possible, but requires a specific combination of factors including high 123Rev Fish Biol Fisheries DOI 10.1007/s11160-016-9445-y abundances of particular species, processing knowledge dictated by the target market, and either inexpensive labor or industrialized processing facilities. More often than not, these factors are not altogether evaluated prior to attempting a new jellyfish fishery. As such, jellyfish fisheries are currently expanding much more rapidly than research on the subject, thereby putting ecosystems and stakeholders' livelihoods at risk.

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What is inside the jar? Forensically informative nucleotide sequencing (FINS) of a short mitochondrial COI gene fragment reveals a high percentage of mislabeling in jellyfish food products

Cited by 58 publications

References 24 publications

DNA and Mini-DNA barcoding for the identification of Porgies species (family Sparidae) of commercial interest on the international market

DNA and Mini-DNA barcoding for the identification of Porgies species (family Sparidae) of commercial interest on the international market

Fish species identification in canned pet food by BLAST and Forensically Informative Nucleotide Sequencing (FINS) analysis of short fragments of the mitochondrial 16s ribosomal RNA gene (16S rRNA)

Jellyfish fisheries in the Americas: origin, state of the art, and perspectives on new fishing grounds

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