What is CRISPR/Cas9?

Redman, Melody; King, Andrew; Watson, Caroline J.; King, David C.

doi:10.1136/archdischild-2016-310459

Cited by 112 publications

(79 citation statements)

References 13 publications

(18 reference statements)

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“…The use of site-speciic DNA endonucleases including Zinc inger nucleases (ZFNs), [161] transcription activator-like efector nucleases (TALENs) [162] and now clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 [163] have equipped researchers with the ability to speciically inactivate genes and target genetic regions for homologous recombination of input DNA. In general, double-stranded breaks introduced by nucleases activates DNA repair mechanisms which generate mutations in the target sequence conferring a loss of expression i.e., gene editing.…”

Section: Host Resistancementioning

confidence: 99%

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

Bernard¹,

Egnin²,

Bonsi³

2017

Nematology - Concepts, Diagnosis and Control

123

101

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Plant-parasitic nematodes are costly burdens of crop production. Ubiquitous in nature, phytoparasitic nematodes are associated with nearly every important agricultural crop and represent a signiicant constraint on global food security. Root-knot nematodes (Meloidogyne spp.) cyst nematodes (Heterodera and Globodera spp.) and lesion nematodes (Pratylenchus spp.) rank at the top of list of the most economically and scientiically important species due to their intricate relationship with the host plants, wide host range, and the level of damage ensued by infection. Limitations on the use of chemical pesticides have brought increasing interest in studies on alternative methods of nematode control. Among these strategies of nonchemical nematode management is the identiication and implementation of host resistance. In addition, nematode genes involved in parasitism represent key targets for the development of control through gene silencing methods such as RNA interference. Recently, transcriptome proiling analyses has been used to distinguish nematode resistant and susceptible genotypes and identify the speciic molecular components and pathways triggered during the plant immune response to nematode invasion. This summary highlights the importance of plant-parasitic nematodes in agriculture and the molecular events involved in plant-nematode interactions.

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Section: Host Resistancementioning

confidence: 99%

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

Bernard¹,

Egnin²,

Bonsi³

2017

Nematology - Concepts, Diagnosis and Control

123

101

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“…Die HK ist somit eine seltene Erbkrankheit, die allerdings für diese Krankheitskategorie vergleichsweise viele Menschen betrifft. Da immer mehr Deutsche ein hohes Lebensalter erreichen und somit lange genug leben, um eine klinische Manifestation der HK auch bei geringen CAG-Triplett-Wiederholungen (36)(37)(38)(39) im HTT-Gen zu erlauben, ist eine Zunahme der Prävalenz wahrscheinlich [4].…”

Section: Hintergrundunclassified

Genselektive Therapieansätze bei der Huntington-Krankheit

et al. 2020

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ZusammenfassungIn Deutschland leiden derzeit mindestens 8000, vermutlich aber sogar bis zu ca. 14.000 Menschen an einer klinisch apparenten („manifesten“) Huntington-Krankheit (HK). Zudem tragen schätzungsweise 24.000 Deutsche die der HK zugrunde liegende Mutation im Huntingtin-(HTT)-Gen und werden im Laufe ihres Lebens an der HK erkranken. Obwohl die HK eine seltene neurodegenerative Erkrankung ist, steht sie gegenwärtig im Fokus eines allgemeinen medizinischen Interesses: Klinische Studien, die eine rationale Basis für die Hoffnung bilden, das bislang unaufhaltsame, schicksalhafte Fortschreiten der Erkrankung bis zur vollständigen Pflegebedürftigkeit bremsen und – bei rechtzeitigem Behandlungsbeginn – eventuell sogar die klinische Manifestation der HK mitigieren zu können, haben begonnen. Diese innovativen Therapieansätze sind darauf ausgerichtet, die Nachbildung mutierter HTT-Gen-Produkte zu hemmen. Eine erste klinische Arzneimittelprüfung zum Nachweis der Wirksamkeit (Phase III) intrathekaler Antisense-Oligonukleotide (ASO, Wirkstoff RG6042) hat 2019 begonnen. Klinische Studien zu weiteren, alternativen Behandlungsansätze mit allelselektiven ASOs sowie zu gentherapeutischen Ansätzen mit RNA-Molekülen und Zinkfinger-Repressor-Komplexen stehen kurz bevor. In dem vorliegenden Artikel geben wir einen Überblick über die gegenwärtig diskutierten genselektiven Therapieansätze bei der HK.

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“…In 2005, various research groups reported that CRISPR was observed in prokaryotes and supported the acquired immunity to viruses (Bolotin et al, 2005;Pourcel et al, The CRISPR-Cas system, originally described as an adaptive immune system in bacteria and archaea, is now designed as RNA-directed endonucleases for genome regulation, enabling rapid, inexpensive and relatively easy correction of errors in the genome. (Ma et al, 2014;Redman et al, 2016). In this technology, programmable nucleases, similar to DNA restriction enzymes, cuts DNA double strands in the region where genome editing is desired.…”

Section: Genome Editing and Crispr-cas9mentioning

confidence: 99%

“…Recently, significant progress has been made in this area. One of the most exciting applications of CRISPR-Cas9 is its use for the treatment of genetic diseases caused by single gene mutations such as Duchenne muscle dystrophy (DMD), cystic fibrosis (CF) and hemoglobinopathies (Dai et al, 2016;Redman et al, 2016). There are several studies related to the in vivo genome editing for the repair of DMD-causing mutations in the dystrophic mouse model published in Science.…”

Section: Genome Editing and Crispr-cas9mentioning

confidence: 99%

İndüklenmiş pluripotent kök hücrelerde CRISPR / Cas9 tabanlı gen düzenleme teknolojileri

Nozhatzadeh¹

2020

genediting

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What is CRISPR/Cas9?

Cited by 112 publications

References 13 publications

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

The Impact of Plant-Parasitic Nematodes on Agriculture and Methods of Control

Genselektive Therapieansätze bei der Huntington-Krankheit

İndüklenmiş pluripotent kök hücrelerde CRISPR / Cas9 tabanlı gen düzenleme teknolojileri

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