What happens to abnormally fertilized embryos? A scoping review

Kemper, James M.; Liu, Yanhe; Afnan, Masoud; Mol, Ben Willem J.; Morbeck, Dean E.

doi:10.1016/j.rbmo.2023.02.005

Cited by 5 publications

(3 citation statements)

References 59 publications

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“…To our knowledge, few studies have compared live birth rates after embryo transfer developed from 1PN in fresh and frozen-thawed cycles. Indeed, a recent review authored by Kemper et al [9], confirmed that most publications concern fresh cycles.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Birth of Thirty-One Healthy Babies Following Transfer of Fresh and Frozen-Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study

Labied,

Wenders,

Gaspard

et al. 2023

Preprint

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Fertilized zygotes normally display 2 pronuclei (PN) but abnormal fertilization patterns (0, 1 or &gt;2 PN) are daily observed in IVF labs. Multiple PN zygotes (&gt;2) are generally discarded due to an increased risk of aneuploidy. However, the decision to transfer or not 1PN-derived embryos remains controversial. The aims of our study were to analyze the neonatal outcomes of fresh or frozen-thawed embryos derived from 1PN zygotes, and to evaluate the influence of the fertilization method. Data were retrospectively collected from cycles performed between January 2018 and December 2022. Fresh cycles were analyzed for the comparative fate of 1PN zygotes (n=1234) following conventional in vitro fertilization (cIVF; n=648) or intracytoplasmic sperm injection (ICSI; n=586), as well as the results of the 64 transfers of 1PN derived embryos (pregnancy rate (PR) and neonatal outcomes). This pregnancy follow-up was also applied to 167 transfers of frozen-thawed 1PN derived embryos. In fresh cycles, 46% of the 1PN zygotes in cIVF group gave rise to embryos of sufficient quality to be transferred or frozen (day 3 or 5/6). This rate decreased to 33% in the fresh ICSI cycles. Blastulation rate was also significantly higher in cIVF group (44%) in comparison to ICSI group (20%). The fresh embryo transfers (32 per group) allowed 7 pregnancies in the cIVF group (PR=21.9%) as compared to 4 pregnancies in the ICSI group (PR=12.5%). In the cIVF group, 4 deliveries of healthy newborns were achieved and only one in the ICSI group. In frozen/thawed cycles, 36 pregnancies were obtained out of the 167 transfers. A non-significative difference was observed between embryos derived from cIVF cycles (PR=26%) and ICSI cycles (PR=16%) with respectively 18 and 8 healthy babies born. In conclusion, we observed better outcomes for 1PN zygotes in cIVF cycles in comparison to ICSI cycles. Our center policy to transfer good quality 1PN-derived embryos allowed the birth of 31 healthy babies.

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Section: Discussionmentioning

confidence: 99%

“…The first aim of our study is to share our experience with 1PNderived embryos and to help answer the question posed by several teams and recently reviewed by M Kemper « what happens to abnormally fertilized embryos ? » [9].…”

Section: Introductionmentioning

confidence: 99%

Birth of Thirty-One Healthy Babies Following Transfer of Fresh and Frozen-Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study

Labied,

Wenders,

Gaspard

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Occasionally, only 1PN is seen in the cytoplasm of the oocyte, and this is generally attributed to oocyte parthenogenetic activation, irregular pronuclear formation resulting from asynchrony of pronuclear appearance, or possibly male and female pronuclear fusion. Zygotes with one PN are thought to be at a higher risk of being haploid, and the transfer of these embryos is expected to result in an implantation failure [1]. Even though the zygotes deriving from abnormally fertilized oocytes are capable of normal in vitro development, they are usually discarded because of a higher risk for abnormal ploidy constitution (i.e., haploidy, triploidy, or tetraploidy) [2].…”

Section: Introductionmentioning

confidence: 99%

Perspective Chapter: Application of Abnormally Fertilized Eggs and the Associated Clinical Outcomes

Wu,

Feng,

et al. 2024

Obstetrics and Gynecology

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Human IVF laboratory often shows many abnormal fertilization eggs, such as no pronucleus (0PN), monopronucleus (1PN), three pronuclei (3PN) or multi-pronuclei (mPN) zygotes and these abnormal eggs are usually not used and typically discarded. Recent researches have showed that further evaluation on these abnormal eggs may provide some hope for aged infertile couples to have babies in their family. Our study showed that 0PN eggs may be rescued by introcytoplasmic sperm injection (ICSI). The 1PN zygotes should be cultured to observe their cleavage and blastocyst formation until Day 3 to Day 6. Selecting normal chromosomal embryo transfer may have healthy baby birth. Although most of 3PN embryos are genetically abnormal chromosomal composition, a small portion of 3PN embryos may develop to blastocyst with normal chromosomal composition. Also, those embryos derived from 3PN, especially by ICSI, have more possibility for self-correction to become normal euploid embryos. The microsurgically removing 1PN from 3PN zygotes may artificially correct this abnormal fertilization. After one PN removal, the formed blastocyst may be screened by the PGT for embryo transfer in rare embryo patients to achieve pregnancy and delivery of a healthy newborn. Based on no obvious difference of ooplasm between normal fertilized 2PN and 3PN zygotes, the cytoplasm of 3PN zygotes may be used to supplement the aged woman poor oocytes to improve embryo quality. Transferring partial cytoplasm from 3PN zygote to the fertilized 2PN zygotes of aged woman may promote the receipt embryo to develop blastocysts. This partial ooplasmic transfer does not change the aging woman genetic composition and the woman embryos still keep her with her husband genetic genes in the cell nucleus. However, the baby born with this technique might appear epigenetics because the mixed mitochondrial DNA would be passed on to all future generations.

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Re-evaluating one pronucleus embryos: expanding opportunities for couples in preimplantation genetic testing

Alteri,

Capalbo,

Pagliardini

2024

Fertility and Sterility

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What happens to abnormally fertilized embryos? A scoping review

Cited by 5 publications

References 59 publications

Birth of Thirty-One Healthy Babies Following Transfer of Fresh and Frozen-Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study

Birth of Thirty-One Healthy Babies Following Transfer of Fresh and Frozen-Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study

Perspective Chapter: Application of Abnormally Fertilized Eggs and the Associated Clinical Outcomes

Re-evaluating one pronucleus embryos: expanding opportunities for couples in preimplantation genetic testing

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