What does the susceptible-dose dependent interpretive category for cefepime in ESBL-producing bacteria?

Menegucci, Thatiany Cevallos; Bastos, Marina de Souza; Viana, Giselle Fukita; Moreira, Rafael Renato Brondani; Garcia, Lourdes Botelho; Cardoso, Celso Luíz; Tognim, Maria Cristina Bronharo

doi:10.1080/1120009x.2015.1120407

Cited by 1 publication

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“…The finding that one K. pneumoniae SDD to cefepime strain was diagnosed as sensitive using the LC-MS/MS test could represent a limitation especially because it is known that a high proportion of K. pneumoniae strains fall into SDD category. 27 Therefore, we consider that further research should be done using several intermediate and SDD strains in combination with incubation in nutrient media to confirm if this is a limitation. This could provide more consistent results that meet the CLSI criteria-compliant MIC testing.…”

Section: Dovepressmentioning

confidence: 99%

<p>Detection of Extended-Spectrum β-Lactamase and Carbapenemase Activity in Gram-Negative Bacilli Using Liquid Chromatography – Tandem Mass Spectrometry</p>

Serafim¹,

Shah²,

Licker³

et al. 2020

IDR

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Purpose Several mass spectrometry-based methods for antimicrobial sensitivity testing have been described in recent years. They offer an alternative to commercially available testing systems which were considered to have disadvantages in terms of cost- and time-efficiency. The aim of this study was to develop an LC-MS/MS-based antibiotic hydrolysis assay for evaluating antimicrobial resistance (AMR) of Gram-negative bacteria. Materials and Methods Four species of Gram-negative bacilli ( Klebsiella pneumoniae, Escherichia coli, Providencia stuartii and Acinetobacter baumannii ) were tested against six antibiotics from three different classes: ampicillin, meropenem, imipenem, ceftazidime, ceftriaxone and cefepime. Bacterial suspensions from each species were incubated with a mixture of the six antibiotics. Any remaining antibiotic following incubation was measured using LC-MS/MS. The results were interpreted using measurements obtained for an E. coli strain sensitive to all antibiotics and expressed as percentage of hydrolyzed antibiotic. These were subsequently compared to commercially-available system for the bacteria identification and susceptibility testing. Results Overall, LC-MS/MS assay and commercial antimicrobial susceptibility platform results showed good agreement in terms of an organism being resistant/sensitive to an antibiotic. The time required to complete the LC-MS/MS-based hydrolysis test was under 5 h, significantly shorter that commercially available susceptibility testing platforms. Conclusion By using a sensitive strain for results interpretation and simultaneous use of multiple antibiotics, the proposed protocol offers improved robustness and multiplexing over previously described methods for antibiotic sensitivity testing. Nevertheless, further research is needed before routine assimilation of the method, especially for strains with intermediate resistance.

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Section: Dovepressmentioning

confidence: 99%