What do we know about plasmids carried by members of the Acinetobacter genus?

Brovedan, Marco; Cameranesi, María Marcela; Limansky, Adriana S.; Morán-Barrio, Jorgelina; Marchiaro, Patricia; Repizo, Guillermo Daniel

doi:10.1007/s11274-020-02890-7

Cited by 25 publications

(34 citation statements)

References 128 publications

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“…Acinetobacter baumannii possesses the ability to develop intrinsic resistance via reducing membrane permeability, efflux pump activity, and the production of wide varieties of ß-lactamases enzymes [11]. However, resistance in this pathogen is frequently associated with mobile genetic elements (MGEs) transferable between bacteria, enabling rapid dissemination and maintenance of resistance genes between different bacterial species [12]. It can also acquire resistance via mutational changes in chromosomal structure and horizontal gene transfer [13], in addition to some different naturally occurring intrinsic resistance genes [14].…”

Section: Introductionmentioning

confidence: 99%

WGS based analysis of acquired antimicrobial resistance in human and non-human Acinetobacter baumannii isolates from a German perspective

et al. 2021

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Background Acinetobacter baumannii ability to develop and acquire resistance makes it one of the most critical nosocomial pathogens globally. Whole-genome sequencing (WGS) was applied to identify the acquired or mutational variants of antimicrobial resistance (AMR) genes in 85 German A. baumannii strains utilizing Illumina technology. Additionally, the whole genome of 104 German isolates deposited in the NCBI database was investigated. Results In-silico analysis of WGS data revealed wide varieties of acquired AMR genes mediating resistance mostly to aminoglycosides, cephalosporins, carbapenems, sulfonamides, tetracyclines and macrolides. In the 189 analyzed genomes, the ant (3″)-IIa conferring resistance to aminoglycosides was the most frequent (55%), followed by blaADC.25 (38.6%) conferring resistance to cephalosporin, blaOXA-23 (29%) and the blaOXA-66 variant of the intrinsic blaOXA-51-likes (26.5%) conferring resistance to carbapenems, the sul2 (26%) conferring resistance to sulfonamides, the tet. B (19.5%) conferring resistance to tetracycline, and mph. E and msr. E (19%) conferring resistance to macrolides. blaTEM variants conferring resistance to cephalosporins were found in 12% of genomes. Thirteen variants of the intrinsic blaOXA-51 carbapenemase gene, blaOXA-510 and blaADC-25 genes were found in isolates obtained from dried milk samples. Conclusion The presence of strains harboring acquired AMR genes in dried milk raises safety concerns and highlights the need for changes in producing dried milk. Acquired resistance genes and chromosomal gene mutation are successful routes for disseminating AMR determinants among A. baumannii. Identification of chromosomal and plasmid-encoded AMR in the genome of A. baumannii may help understand the mechanism behind the genetic mobilization and spread of AMR genes.

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Section: Introductionmentioning

confidence: 99%

WGS based analysis of acquired antimicrobial resistance in human and non-human Acinetobacter baumannii isolates from a German perspective

et al. 2021

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“…A number of complete bacterial plasmid genomes has increased many times in recent years and continues to grow rapidly (Shintani et al, 2015;Lean and Yeo, 2017;Brovedan et al, 2019Brovedan et al, , 2020. Against this background, studies of their structure and functioning are noticeably lagging.…”

Section: Introductionmentioning

confidence: 99%

“…An important feature of Acinetobacter is also the presence of multiple plasmids in the same strain. In an attempt to study the diversity and prevalence of plasmids in different Acinetobacter species, various researchers tried to classify them using approaches based on a comparative analysis of the structure of replication initiator proteins ( Bertini et al, 2010 ; Lean and Yeo, 2017 ; Cameranesi et al, 2017 ; Salto et al, 2018 ; Brovedan et al, 2020 ) or the structure of mobilization proteins (relaxases; Francia et al, 2004 ; Garcillán-Barcia et al, 2009 ; Smillie et al, 2010 ; Garcillán-Barcia and de la Cruz, 2013 ; Shintani et al, 2015 ; Brovedan et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Acinetobacter Plasmids: Diversity and Development of Classification Strategies

et al. 2020

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Bacteria of the genus Acinetobacter , with their numerous species common in various habitats, play a significant role as pathogens. Their ability to adapt to different living conditions is largely due to the presence of numerous plasmids containing the necessary adaptive genes. At the same time the diversity of Acinetobacter plasmids and their evolutionary dynamics have not been sufficiently studied. Here, we characterized 44 plasmids isolated from five permafrost Acinetobacter lwoffii strains, examined their relationship with plasmids of modern Acinetobacter strains and identified groups of related plasmids. For this purpose, we have developed a combined approach for classifying all known Acinetobacter plasmids. The classification took into account the size of plasmids, the presence and structure of the rep and mob genes, as well as the structure of their backbone and accessory regions. Based on the analysis, 19 major groups (lineages) of plasmids were identified, of which more than half were small plasmids. The plasmids of each group have common features of the organization of the backbone region with a DNA identity level of at least 80%. In addition, plasmids of the same group have similarities in the organization of accessory regions. We also described a number of plasmids with a unique structure. The presence of plasmids in clinical strains that are closely related to those of environmental permafrost strains provides evidence of the origin of the former from the latter.

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“…Self-transmissible plasmids by conjugation must encode a complete set of components required for transfer such as a relaxase (Mob protein), and the type IV secretion system (T4SS) ( 15 – 17 ). Here, we show that pHUAV/OXA-24/40 encodes the relaxase protein MobA, but no other conjugation-related genes, and is thus a mobilizable but not self-transmissible plasmid ( Fig.…”

Section: Observationmentioning

confidence: 99%

Carbapenem Resistance in Acinetobacter nosocomialis and Acinetobacter junii Conferred by Acquisition of bla _OXA-24/40 and Genetic Characterization of the Transmission Mechanism between Acinetobacter Genomic Species

et al. 2022

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What do we know about plasmids carried by members of the Acinetobacter genus?

Cited by 25 publications

References 128 publications

WGS based analysis of acquired antimicrobial resistance in human and non-human Acinetobacter baumannii isolates from a German perspective

WGS based analysis of acquired antimicrobial resistance in human and non-human Acinetobacter baumannii isolates from a German perspective

Acinetobacter Plasmids: Diversity and Development of Classification Strategies

Carbapenem Resistance in Acinetobacter nosocomialis and Acinetobacter junii Conferred by Acquisition of bla _OXA-24/40 and Genetic Characterization of the Transmission Mechanism between Acinetobacter Genomic Species

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What do we know about plasmids carried by members of the Acinetobacter genus?

Cited by 25 publications

References 128 publications

WGS based analysis of acquired antimicrobial resistance in human and non-human Acinetobacter baumannii isolates from a German perspective

WGS based analysis of acquired antimicrobial resistance in human and non-human Acinetobacter baumannii isolates from a German perspective

Acinetobacter Plasmids: Diversity and Development of Classification Strategies

Carbapenem Resistance in Acinetobacter nosocomialis and Acinetobacter junii Conferred by Acquisition of bla OXA-24/40 and Genetic Characterization of the Transmission Mechanism between Acinetobacter Genomic Species

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Carbapenem Resistance in Acinetobacter nosocomialis and Acinetobacter junii Conferred by Acquisition of bla _OXA-24/40 and Genetic Characterization of the Transmission Mechanism between Acinetobacter Genomic Species