We photoionize laser-cooled atoms with a laser beam possessing spatially periodic intensity modulations to create ultracold neutral plasmas with controlled density perturbations. Laser-induced fluorescence imaging reveals that the density perturbations oscillate in space and time, and the dispersion relation of the oscillations matches that of ion acoustic waves, which are long-wavelength, electrostatic, density waves.Collective wave phenomena are central to the transport and thermodynamic properties of plasmas, and the presence of a rich spectrum of collective modes is a distinctive feature that separates this state of matter from a simple collection of charged particles [1]. In ultracold neutral plasmas (UNPs) [2,3], which are orders of magnitude colder than any other neutral plasma and can be used to explore the physics of strongly coupled systems [4][5][6], little work has been done to study collective modes [7][8][9][10]. Here we employ a new technique for creating controlled density perturbations to excite ion acoustic waves (IAWs) in an UNP and measure their dispersion relation. This flexible technique for sculpting the density distribution will open new areas of plasma dynamics for experimental study, including the effects of strong coupling on dispersion relations [11][12][13][14] and non-linear phenomena [3,10,15,16] in the ultracold regime.UNPs are formed by photoionizing laser-cooled atoms near the ionization threshold. They stretch the boundaries of traditional neutral plasma physics and have extremely clean and controllable initial conditions that make them ideal for studying phenomena seen in more complex systems, such as plasma expansion and equilibration in high-energy-density laser-matter interactions [5] and quark-gluon plasmas [6]. UNPs have shown fascinating dynamics, such as kinetic energy oscillations that directly reflect the strong coupling of ions [5,17]. Strong coupling arises when particle interaction energies exceed the kinetic energy [4]. It is important in many fields of physics spanning classical to quantum behavior [5,6,18,19] and gives rise to phase transitions and the establishment of spatial correlations of particles [4]. These studies complement experiments probing strong coupling in dusty plasmas [18] and non-neutral plasmas of pure ions or electrons [19].Previous experimental studies of collective modes in UNPs were limited to excitations of Langmuir (electron density) oscillations with radio frequency electric fields [7,8] which did not determine a dispersion relation and were relatively insensitive to dynamics of the strongly coupled ions. A high-frequency electron drift instability was observed in an UNP in the presence of crossed electric and magnetic fields [9]. Spherically symmetric ion density modulations were shown to excite IAWs in numerical simulations of UNPs [10]. Here we excite IAWs through direct imprinting of ion density modulations during plasma formation and image them in situ with time resolved laser-induced fluorescence [20].Low frequency electrostatic, o...