What can the Allium cepa test say about pesticide safety? A review

Cotrim, Carlos Filipe Camilo; Bailão, Elisa Flávia Luiz Cardoso; Ondei, Luciana Souza; Carneiro, Fernanda Melo; Almeida, Luciane Madureira

doi:10.1007/s11356-022-20695-z

Cited by 16 publications

(7 citation statements)

References 92 publications

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“…No obstante, esta especie se encuentra prohibida por el ANMAT (2001) ya que presenta actividad genotóxica, al igual que Dysphania ambrosioides (L.) Mosyakin & Clemants, utilizada con fines similares, para el sistema digestivo, por ambas comunidades originarias y también como antiparasitario en La Represita. En relación con el uso de especies que pueden ocasionar daños a la salud, durante uno de los talleres se informó a los pobladores sobre sus efectos nocivos, particularmente aquellos genotóxicos, mediante la visualización de imágenes de células meristemáticas de ápices de raíces adventicias de Allium cepa L., "cebolla", a las que se les realizó el test de Allium cepa L. (Camilo-Cotrim et al, 2022). Esta información fue incluida, junto con todas las actividades y otros aspectos etnobotánicos desarrollados en los talleres, en un documento didáctico que se entregó a los docentes de la escuela local.…”

Section: íNdices Etnobotánicos (Tabla 1)unclassified

El acervo etnobotánico medicinal del Pueblo Nación Huarpe de San Luis (La Represita, San Luis, Argentina)

Moglia,

Isaguirre,

Cangiano

2023

Bol. Soc. Argent. Bot.

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Introducción y objetivos: El patrimonio etnobotánico de la comunidad Huarpe aún no ha sido documentado y podría llegar a perderse. El objetivo del trabajo fue recopilar el conocimiento etnobotánico medicinal del Pueblo Nación Huarpe de San Luis (Argentina) del paraje La Represita, a través de encuentros dialógicos entre los integrantes de la comunidad y la universidad. M&M: El trabajo de campo incluyó entrevistas semi-estructuradas y encuestas a 31 pobladores locales, tres talleres con la comunidad educativa local y dos encuentros abiertos a toda la población. Se realizó un análisis de los usos medicinales de los recursos vegetales locales, que incluyó el cálculo de índices etnobotánicos: Frecuencia relativa de citación, Nivel de Fidelidad, Valor de Uso e Índice de Novedad. Resultados: El número de taxones utilizados por los pobladores con fines medicinales fue de 48 y las partes más utilizadas de las plantas fueron las hojas y tallos, principalmente en forma de infusión. A través del cálculo de los índices etnobotánicos, se obtuvo que las plantas aromáticas fueron particularmente importantes como recursos medicinales. Se registraron 14 categorías etic medicinales y tres veterinarias, las más destacadas fueron las relacionadas con trastornos digestivos y respiratorios humanos. Conclusiones: Los pobladores huarpes de San Luis, particularmente las mujeres, atesoran importantes conocimientos sobre plantas medicinales que forman parte de las acciones de su vida diaria. Por esta razón, es importante seguir investigando a través de nuevos estudios que incluyan a los pobladores de la comunidad Huarpe Pinkanta de San Luis.

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Section: íNdices Etnobotánicos (Tabla 1)unclassified

El acervo etnobotánico medicinal del Pueblo Nación Huarpe de San Luis (La Represita, San Luis, Argentina)

Moglia,

Isaguirre,

Cangiano

2023

Bol. Soc. Argent. Bot.

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“…The Allium cepa model stands out from the other models used in toxicology because of its specific characteristics, which enhance the general advantages of the plant models themselves. The dimension and number of its chromosomes (2 n = 16) allow the analysis of cytotoxicological (mitotic index) and genotoxicological parameters (chromosomal aberrations, micronuclei, DNA damage) (Camilo‐Cotrim et al, 2022; Felisbino et al, 2018; Gupta et al, 2022; Ragazzo et al, 2017; Tedesco & Laughinghouse IV, 2012). In addition, Allium cepa is readily available as seeds and bulbs, making it possible to evaluate the efficacy of samples on two vegetative forms of the same organism.…”

Section: Introductionmentioning

confidence: 99%

A scoping review of recent advances in the application of comet assay to Allium cepa roots

Alias

Zerbini

Feretti

2023

Environ and Mol Mutagen

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The comet assay is a sensitive method for the evaluation of DNA damages and DNA repair capacity at single‐cell level. Allium cepa is a well‐established plant model for toxicological studies. The aim of this scoping review was to investigate the recent application of the comet assay in Allium cepa root cells to assess the genotoxicity. To explore the literature a search was performed selecting articles published between January 2015 and February 2023 from Web of Science, PubMed, and Scopus databases using the combined search terms “Comet assay” and “Allium cepa”. All the original articles that applied the comet assay to Allium cepa root cells were included. Of the 334 records initially found, 79 articles were identified as meeting the inclusion criteria. Some studies reported results for two or more toxicants. In these cases, the data for each toxicant were treated separately. Thus, the number of analyzed toxicants (such as chemicals, new materials, and environmental matrices) was higher than the number of selected papers and reached 90. The current use of the Allium‐comet assay seems to be directed towards two types of approach: the direct study of the genotoxicity of compounds, mainly biocides (20% of analyzed compounds) and nano‐ and microparticles (17%), and assessing a treatment's ability to reduce or eliminate genotoxicity of known genotoxicants (19%). Although the genotoxicity identified by the Allium‐comet assay is only one piece of a larger puzzle, this method could be considered a useful tool for screening the genotoxic potential of compounds released into the environment.

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“…One of the possibilities for studying the genotoxic effect of pesticides is using plants as test objects. Plants are recognized as excellent genetic models in detecting mutagenic environmental agents and are often used for biomonitoring studies [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic Effects of the Neonicotinoid Insecticides Nuprid 200 SL and Calypso 480 SC on Plant Model System

Stoyanov,

Vasileva,

Staykova

et al. 2023

OBM Genet

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Data from the present study indicate that the neonicotinoid insecticides Nuprid 200 SL (with the active substance imidacloprid) and Calypso 480 SC (with the active substance thiacloprid) reduce the mitotic activity by inhibiting cell division and increasing chromosomal aberrations in the cells of the root apical meristem of Allium cepa L. The tested solutions of pesticides cause a wide range of anomalies associated with disorders in forming the cell's division apparatus and the integrity of chromosomes. The comparative analysis of their mutagenic action shows a higher genotoxic potential of Calypso 480 SC. The established indices of chromosomal abnormalities for Calypso 480 SC and Nuprid 200 SL are 1.07 ± 0.38% and 0.70 ± 0.41% respectively. The mitosodepressant effect and a large number of chromosomal aberrations and mitotic abnormalities in Allium cepa cells when treated with Nuprid 200 SL and Calypso 480 SC are evidence of their high toxic potential and the significant risk of environmental pollution by their use in agriculture. Parts of this work was published in abstract form and presented as an oral presentation at the IV International Agricultural, Biological & Life Science conference, Edirne, Turkey, 2022 August 29-31, [1] and as a poster presentation at the 5th Balkan Scientific Conference on Biology, Plovdiv, Bulgaria, 2021 April 15-16 [2].

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What can the Allium cepa test say about pesticide safety? A review

Cited by 16 publications

References 92 publications

El acervo etnobotánico medicinal del Pueblo Nación Huarpe de San Luis (La Represita, San Luis, Argentina)

El acervo etnobotánico medicinal del Pueblo Nación Huarpe de San Luis (La Represita, San Luis, Argentina)

A scoping review of recent advances in the application of comet assay to Allium cepa roots

Cytogenetic Effects of the Neonicotinoid Insecticides Nuprid 200 SL and Calypso 480 SC on Plant Model System

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