What can be measured with rast?

Knowledge of specific IgE bound on immunosorbent as assayed by RAST is useful but cannot give a complete description of the biological states of patients concerning IgE antibodies. Using a computer program based on a least squares error method and knowing the amount of bound specific IgE antibodies to grass pollen antigens in a serum at each of the four tested dilutions it is possible to calculate the absolute value of specific IgE antibody level, the relative value of affinity constant and the relative proportion of IgE antibodies to the total amount of specific (IgE plus non-IgE) antibodies. It is found that specific IgE antibodies account for high percentages of the total IgE protein (mean of 20%). High differences in affinity constant and in the proportion of IgE antibodies to the total amount of antibodies could be found between sera. Severity of grass pollen allergy could be related to the absolute level of IgE antibody, but no definite conclusion could be made on the importance of affinity constant and the proportion of IgE antibodies to the total amount of antibodies.

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“…Levels of IgE antibodies in patients were assayed, and Table 2 shows the different (10) 45.6 (11) 58.8 (9) 64.8 (8) 67.2 (7) 76.8 (5)…”

Section: Resultsmentioning

confidence: 99%

“…The basic principles of RAST have been studied in previous papers (5,21) in order to provide a method which takes into account most of the equilibrium factors and uses more refined assumptions for the study and quantification of specific IgE andbodies.…”

mentioning

confidence: 99%

Absolute Determination of IgE Antibodies to Grass Pollen Allergens

Vervloët

Bongrand

Charpin

1978

Allergy

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“…The antigen may have many different determinants, each with its own specific antibody population and antibodies of low affinity may be present in large amounts (Kim et al, 1974). Even the simplest approach to the quantitation of one antibody (IgE) in a serum requires several measurements to be made to take account of the equilibrium between free antibody and that bound to allergen (Bongrand et al, 1976). The amount of one antibody bound to an insoluble antigen is thus influenced by the experimental conditions chosen and is not determined solely by the total amount present.…”

Section: Discussionmentioning

confidence: 99%

Estimation of IgM rheumatoid factors by fluorimetry.

Lea¹,

Ward²

1978

Annals of the Rheumatic Diseases

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Stock borate saline buffer (Kotoulas and Moroz' 1971) containing 0O15 M sodium chloride and O-15 M boric acid was adjusted to pH 8 6 with sodium hydroxide and used as a 10% solution. Stock potassium phosphate buffer pH 7, 1 mol/l containing sodium azide (2 g/l) was diluted to 0-1 mol/l for use and Tween 20 (0 05%) was added to reduce nonspecific binding effects (Engvall and Perlmann, 1972). A stock solution (10 mg/ml) of rabbit FIl in phosphate buffer was prepared and filtered for use. It was stored at -20°C. Alkaline sodium dodecyl sulphate (SDS) was prepared using 2 g/100 ml 0 -1 N NaOH. MethodUsing a 1 cm diameter quartz cell in a Locarte digital fluorimeter with 13 stage PM tube and zinc lamp, the optimum sensitivity was achieved with a primary filter combination 805 Q (Ilford) and BG 12/2 mm (Schott) and secondary filters 805 Q, OG 515/2 mm, and Wratten 55 (Kodak). The instrument response was linear, turbidity interference minimal, and stability within experiments and between days high after a few minutes' warm-up. After treatment with ethanol and hot 40 % caustic soda solution, the plastic discs were rinsed 247 on 6 April 2019 by guest. Protected by copyright.

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