Wharton jelly stem cells inhibits AGS gastric cancer cells through induction of apoptosis and modification of MAPK and NF-κB signaling pathways

Abbasi, Samaneh; Bazyar, Reza; Saremi, Mohammad Ali; Alishiri, Gholamhoseen; Seyyedsani, Nasrin; Jadid, Mahdieh Farhoudi Sefidan; Khorrami, Afshin; Golmarz, Parisa Emami; Jahangirzadeh, Gholamreza; Bedoustani, Ahmad Babazadeh; Isazadeh, Alireza; Hajazimian, Saba; Amoodizaj, Fatemeh Firouzi

doi:10.1016/j.tice.2021.101597

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…A higher expression of BCL2, on the other hand, inhibits apoptosis and causes cell survival (4). Therefore, the regulation of BAX and BCL2 gene expression plays a strategic role in cancer therapy (31,32). A study by Esmaeili and Farimani suggested that the S. sahandica extraction significantly contributed to the upregulation in the expression of BAX as well as to the downregulation in the expression of BCL2 in the treated breast cancer cell (33).…”

Section: Discussionmentioning

confidence: 99%

Anti-proliferative Effect of Satureja sahandica Extraction by Co-administration of Layered Double Hydroxide (LDH) Nanosheets on HepG2 Hepatocellular Carcinoma Cell Line

Abdol,

Taghipour,

Raeghi

et al. 2023

Gene Cell Tissue

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Background: Hepatocellular carcinoma (HCC) is a common solid tumor and the third cause of cancer death worldwide. Resistance to chemotherapeutic drugs is a serious challenge in the treatment of HCC. To address this challenge, therefore, attempts have been made to develop novel anticancer agents by using medicinal plants and producing novel natural chemical compounds. Objectives: This study aimed to investigate the anti-proliferative effect of the Satureja sahandica extraction combined with layered double hydroxide (LDH) nanosheets on the HepG2 HCC cell line. Methods: The anti-proliferative effects of S. sahandica extraction and LDH nanosheets on HCC cell line HepG2 were investigated by adopting the MTT method. The morphological alterations, migration, and apoptosis rates were also evaluated in the treated cancer cells. The apoptosis-related genes mRNA expressions (BAX and BCL2) were examined by performing real-time PCR. Results: According to the study results, the S. sahandica extraction treatments significantly decreased the viability and proliferation of the HCC cell line HepG2. However, the anticancer activity of the S. sahandica extraction and LDH nanosheet combination was significantly less than its monotherapy. Various morphological alterations and apoptosis rates increased in the cancer cells. However, the migration rate of the cancer cells significantly decreased. Furthermore, the expressions of BAX and BCL2 genes were upregulated and downregulated, respectively. Conclusions: In sum, the significant anti-proliferative activity of the S. sahandica extraction against the HCC cells was documented, which may have been due to the induction of apoptosis. However, LDH nanosheets decreased the anti-proliferative activity of S. sahandica extraction.

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Section: Discussionmentioning

confidence: 99%

Anti-proliferative Effect of Satureja sahandica Extraction by Co-administration of Layered Double Hydroxide (LDH) Nanosheets on HepG2 Hepatocellular Carcinoma Cell Line

Abdol,

Taghipour,

Raeghi

et al. 2023

Gene Cell Tissue

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“…Moreover, many studies have been performed to introduce novel natural compounds and derivatives as potential chemotherapeutic agents. In particular, pharmacological studies have significantly focused on various herbal medicine and bioactive compounds over the past two decades (24,25). This special attention to the natural compounds and herbal medicine is due to their low side effects, high efficiency, low costs, as well as targeting of cancer-related signaling pathways (26, Hydroxytyrosol is one of the most notable natural compounds which has been introduced in recent years due to its beneficial properties.…”

Section: Discussionmentioning

confidence: 99%

Anti-proliferative Effects of Hydroxytyrosol Against Breast Cancer Cell Lines Through Induction of Apoptosis

et al. 2022

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Background: Due to high mortality of patients with metastatic breast cancer and limited strategies for management of this malignancy, development of novel therapeutic approaches and anti-cancer agents is essential. Objectives: In the present study, we investigated in vitro anti-proliferative effects of hydroxytyrosol, as a natural chemotherapeutic agent, against breast cancer cells MDA-MB-231 and MCF-7. Methods: The anti-proliferation activity of hydroxytyrosol on both MDA-MB-231 and MCF-7 breast cancer cells was evaluated by MTT assay. Apoptosis percentage was assessed by flow cytometry in the treated and untreated cancer cells. Moreover, the expression of three apoptotic genes (BAX, BCL2, and CASP3) was evaluated by Real-Time PCR in the treated cancer cells. Results: Our results indicated that hydroxytyrosol exerted an appropriate anti-proliferation activity on both MDA-MB-231 and MCF-7 cancer cells in a dose- and time-dependent manner. We observed a significant increase in apoptosis percentage in both treated cancer cells compared with untreated controls. In addition, hydroxytyrosol upregulated pro-apoptotic BAX and CASP3 genes while downregulated anti-apoptotic BCL2 gene. Conclusions: The findings of the present study suggested an appropriate anti-proliferation effect by hydroxytyrosol that may be due to apoptosis induction through modification of expression of apoptotic genes in breast cancer cells.

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“…Combination therapy is one of the effective therapeutic strategies that simultaneously target multiple therapeutic anticancer pathways, such as apoptosis induction, cell cycle arrest, potential metastasis reduction, and cancer stem cell inhibition. [6,7] Doxorubicin (DOX) is one of the most common chemotherapeutic agents for several solid cancers, whether alone or in combination with other drugs or therapeutic approaches. This chemotherapy agent belongs to the anthracycline antibiotics family and is extracted from a type of mutant Streptomyces peucetius strain.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Oleanolic acid increases the anticancer potency of doxorubicin in pancreatic cancer cells

Esmaeili,

Nasrollahzadeh Sabet,

Mosaed

et al. 2023

J Biochem & Molecular Tox

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Combination therapy is a novel cancer therapy approach that combines two or more chemotherapy drugs. This treatment modality enhances the efficacy of chemotherapy by targeting key pathways in an additive or synergistic manner. Therefore, we investigated the efficacy of combination therapy by widely used chemotherapy drug doxorubicin (DOX) and oleanolic acid (OA) to induction of apoptosis for pancreatic cancer (PC) therapy. The effects of DOX, OA, and their combination (DOX‐OA) were investigated on proliferation and viability of PC cell line (PANC‐1) by MTT assay. Moreover, migration and invasion of the cancer cells were evaluated by trans‐well migration assay and wound healing assay. Flow cytometry and DAPI (4′,6‐diamidino‐2‐phenylindole) staining were employed to investigate apoptosis quantification and qualification of the treated cancer cells. Finally, mRNA expression of apoptosis‐related genes was assessed by quantitative real‐time polymerase chain reaction. Our results demonstrated that the proliferation and metastasis potential of PC cells significantly decreased after treatment by DOX, OA, and DOX‐OA. Moreover, we observed an increase in apoptosis percentage in the treated cancer cells. The apoptosis‐related gene expression was modified to increase the apoptosis rate in all of the treatment groups. However, the anticancer potency of DOX‐OA combination was significantly more than that of DOX and OA treatments alone. Our study suggested that DOX‐OA combination exerts more profound anticancer effects against PC cell lines than DOX or OA monotherapy. This approach may increase the efficiency of chemotherapy and reduce unintended side effects by lowering the prescribed dose of DOX.

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Wharton jelly stem cells inhibits AGS gastric cancer cells through induction of apoptosis and modification of MAPK and NF-κB signaling pathways

Cited by 7 publications

References 28 publications

Anti-proliferative Effect of Satureja sahandica Extraction by Co-administration of Layered Double Hydroxide (LDH) Nanosheets on HepG2 Hepatocellular Carcinoma Cell Line

Anti-proliferative Effect of Satureja sahandica Extraction by Co-administration of Layered Double Hydroxide (LDH) Nanosheets on HepG2 Hepatocellular Carcinoma Cell Line

Anti-proliferative Effects of Hydroxytyrosol Against Breast Cancer Cell Lines Through Induction of Apoptosis

Oleanolic acid increases the anticancer potency of doxorubicin in pancreatic cancer cells

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