WH1fungin a surfactin cyclic lipopeptide is a novel oral immunoadjuvant

Gao, Ziyan; Zhao, Xiuyun; Lee, Song; Li, Jingjing; Liao, Hao; Zhou, Xiaohui; Wu, Jie; Qi, Gaofu

doi:10.1016/j.vaccine.2013.04.028

Cited by 25 publications

(27 citation statements)

References 30 publications

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“…In recent years, surfactin has been characterized as anti-bacterial, anti-viral, anti-fungal, biosurfactant, immunoadjuvant, anti-tumor, antimycoplasma and hemolytic activities. The potential applications of surfactin are therapeutic applications and environmental applications (Seydlova & Svobodova 2008;Gao et al 2013;Chen et al 2015). Little information is available about surfactin application in feed of aquatic animals.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary surfactin supplementation on growth performance, intestinal digestive enzymes activities and some serum biochemical parameters of tilapia (Oreochromis niloticus) fingerlings

Zhai

Shi

Chen

2016

Italian Journal of Animal Science

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This trial was conducted to investigate the effects of supplementary surfactin on growth performance, intestinal digestive enzymes activities and some serum biochemical parameters of tilapia (Oreochromis niloticus) fingerlings. Three hundred and twenty fish were randomly divided into four treatment groups with four replicates in each group and 20 fish in each replicate. The dietary surfactin levels of four treatment groups were 0 (control group), 50, 100 and 200 mg/kg. The trial period was 7 weeks. Compared with the control group, final body weight, daily feed intake, weight gain rate, feed conversion ratio and protein efficiency ratio were improved significantly by surfactin supplementation (p < 0.05). No significant differences of survival rate were found between the control group and all surfactin supplementation groups (p > 0.05). The activities of protease and lipase in intestine of surfactin supplementation groups were significantly higher than those of the control group (p < 0.05), the amylase activities were similar among all treatment groups (p > 0.05). The activities of aspartate aminotransferase and alanine aminotransferase were decreased significantly by dietary surfactin supplementation (p < 0.05), the levels of blood urea nitrogen, triglyceride, total cholesterol and low-density lipoprotein cholesterol were also decreased significantly (p < 0.05), while lysozyme activity and the high-density lipoprotein cholesterol level were increased significantly by the surfactin supplementation (p < 0.05). In conclusion, we demonstrated a promotion of growth performance and improvement of intestinal digestive enzymes activities and some serum biochemical parameters of tilapia fingerlings by dietary surfactin supplementation.ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Effects of dietary surfactin supplementation on growth performance, intestinal digestive enzymes activities and some serum biochemical parameters of tilapia (Oreochromis niloticus) fingerlings

Zhai

Shi

Chen

2016

Italian Journal of Animal Science

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“…In vivo assay also showed surfactin can reduce the acute inflammation of septic shock induced by lipopolysaccharide (LPS) in rats [15]. We isolated and characterized a WH1fungin surfactin secreted by B. amyloliquefaciens WH1 with immunoadjuvant activities, which can induce a Th2-predominant immune response in vivo [16][17][18]. Based on anti-inflammation role of surfactin, we detect whether WH1fungin could inhibit chronic T1DM in this study.…”

Section: Introductionmentioning

confidence: 91%

“…The total IgG, IgG1 and IgG2a titers in serum were determined by an end-point ELISA assay [17,18]. Briefly, the mice serum were serially diluted from 1:100 for coating ELISA plates at 100 l per well, blocked with 2% bovine serum albumin (BSA), reacted with horseradish peroxidase (HRP) labeled rabbit anti-mouse IgG, IgG1 or IgG2a antibodies (Invitrogen, USA), developed with 3,3 ,5,5 -tetramethylbenzidine (TMB) substrate, and then the reaction was stopped by addition of 50 l, 2 M H 2 SO 4 for detecting OD 450nm value by a microplate reader (BioTek, USA).…”

Section: Determination Of Blood Glucose Serum Insulin and Antibodiesmentioning

confidence: 99%

“…The splenocytes proliferation response to concanavalin A (Con A) was assayed by a routine method [18]. Briefly, 5 × 10 5 splenocytes in 200 l RPMI 1640 medium were incubated with Con A (10 g/ml) (Sigma, USA) in 96 well tissue culture plates at 37 • C and 5% CO 2 for 48 h, then 20 l 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (5 mg/ml) was added for further incubation for 4 h. After removing the medium, 200 l dimethylsulfoxide was added and the absorbance at 490 nm was measured by a plate reader (BioRad, USA).…”

Section: T Cells Proliferation Assaymentioning

confidence: 99%

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Immunomodulation therapy of diabetes by oral administration of a surfactin lipopeptide in NOD mice

Gao

Zhao

Yang

et al. 2014

Vaccine

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“…Previously, we found surfactin exhibiting excellent oral immunoadjuvant activity by protecting protein antigens from pepsin and trypsin degradation in the GI tract, and improving the uptake of antigens by the intestinal epithelial cells (Gao et al, 2013). Otherwise, surfactin is verified safety for oral administration in animals (Hwang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

A natural lipopeptide of surfactin for oral delivery of insulin

et al. 2016

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Surfactin, a natural lipopeptide produced by Bacillus, is gaining attention for potentially biomedical and pharmaceutical applications. Here, surfactin was assayed for oral delivery of insulin (INS) by its ability to bind to and promote protein to penetrate through the cell membrane. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, surfactin was found to form co-precipitates with INS to protect it from acidic and enzymatic attack in the gastrointestinal tract. Further analysis by non-reductive electrophoresis showed surfactin could bind to INS forming heteropolymers. Analysis with circular dichroism, we found this binding significantly influenced the INS structure with decreased rigid a-helix and b-turn, but with increased flexible b-sheet and random coil. The change with more flexible structure was favorable for INS to penetrate through the cell membrane. Fluorescence spectra analysis also showed surfactin could lead Phe and Tyr in the inner of INS exposed outside, further promoting INS permeabilization by improving the hydrophobic-lipophilic interactions between INS and cell membrane. As a result, the effective permeability (Peff) of INS plus surfactin was 4.3 times of that of INS alone. In vivo assay showed oral INS with surfactin displayed excellent hypoglycemic effects with a relative bioavailability of 12.48% and 5.97% in diabetic mice and non-diabetic dogs, respectively. Summary, surfactin is potential for oral delivery of INS by its role as an effective protease inhibitor and permeability enhancer.

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WH1fungin a surfactin cyclic lipopeptide is a novel oral immunoadjuvant

Cited by 25 publications

References 30 publications

Effects of dietary surfactin supplementation on growth performance, intestinal digestive enzymes activities and some serum biochemical parameters of tilapia (Oreochromis niloticus) fingerlings

Effects of dietary surfactin supplementation on growth performance, intestinal digestive enzymes activities and some serum biochemical parameters of tilapia (Oreochromis niloticus) fingerlings

Immunomodulation therapy of diabetes by oral administration of a surfactin lipopeptide in NOD mice

A natural lipopeptide of surfactin for oral delivery of insulin

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