WGBS combined with RNA-seq analysis revealed that Dnmt1 affects the methylation modification and gene expression changes during mouse oocyte vitrification

Ma, Yuzhen; Long, Chunshen; Liu, Gang; Bai, Hongmei; Ma, Li; Bai, Taji; Zuo, Yongchun; Li, Shubin

doi:10.1016/j.theriogenology.2021.09.032

Cited by 14 publications

(12 citation statements)

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“…The developmental competence of the oocyte depends on a well-orchestrated combination of storage and degradation of RNA, in which both over- or under-degradation may affect embryonic development [ 63 , 64 ]. Oocyte vitrification has been mainly linked with the downregulation of mRNAs [ 48 , 50 , 52 , 53 ], which might be correlated with the decrease in stored mRNA found in human oocytes after vitrification [ 74 ]. This is in line with our results for the VIM group, but not for VMAT, in which an equal number of mRNAs were up- and downregulated, compared to the fresh control group.…”

Section: Discussionmentioning

confidence: 99%

“…Comparisons in humans and mice between fresh and vitrified in vivo MII oocytes resulted in pronounced discrepancies between studies. Whereas a first study in mice [ 51 ] showed minor or no changes in the transcriptome, a recent study reported 4747 DE genes [ 50 ]. Similarly, in humans, Monzo et al (2012) [ 48 ] found 608 DE genes using GeneChip arrays, and later, Huo et al (2021) [ 75 ] and Barberet et al (2022) [ 49 ] found notable differences, with a total of 1987 and 108 DE genes, respectively, using single-cell RNA-seq.…”

Section: Discussionmentioning

confidence: 99%

“…Many approaches, such as the use of antioxidants [ 8 ], ice-blockers [ 41 ], different mixtures of cryoprotectants [ 7 , 42 ], and an increased cooling rate [ 3 ] have been tried to optimize oocyte vitrification, but the achieved outcomes are still unsatisfactory. Different studies have reported differential expression in development-related genes after vitrification [ 43 , 44 , 45 , 46 ] and the transcriptomes of vitrified vs. fresh oocytes have been reported in humans [ 47 , 48 , 49 ], mice [ 50 , 51 ], cattle [ 52 , 53 , 54 ], and porcine [ 55 ]. To the best of our knowledge, no transcriptomics studies have been performed on the vitrified oocytes of horses.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation

Angel‐Velez

Meese

Hedia

et al. 2023

IJMS

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In the last decade, in vitro embryo production in horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. Cryopreservation impairs the oocyte developmental potential, which may be reflected in the messenger RNA (mRNA) profile. Therefore, this study aimed to compare the transcriptome profiles of metaphase II equine oocytes vitrified before and after in vitro maturation. To do so, three groups were analyzed with RNA sequencing: (1) fresh in vitro matured oocytes as a control (FR), (2) oocytes vitrified after in vitro maturation (VMAT), and (3) oocytes vitrified immature, warmed, and in vitro matured (VIM). In comparison with fresh oocytes, VIM resulted in 46 differentially expressed (DE) genes (14 upregulated and 32 downregulated), while VMAT showed 36 DE genes (18 in each category). A comparison of VIM vs. VMAT resulted in 44 DE genes (20 upregulated and 24 downregulated). Pathway analyses highlighted cytoskeleton, spindle formation, and calcium and cation ion transport and homeostasis as the main affected pathways in vitrified oocytes. The vitrification of in vitro matured oocytes presented subtle advantages in terms of the mRNA profile over the vitrification of immature oocytes. Therefore, this study provides a new perspective for understanding the impact of vitrification on equine oocytes and can be the basis for further improvements in the efficiency of equine oocyte vitrification.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation

Angel‐Velez

Meese

Hedia

et al. 2023

IJMS

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“…It was found that oocyte vitrification can significantly affect the imprinted genes of oocytes and embryos in mouse, bovine and other animals (Table 1 ). After the vitrification and warming of mouse MII oocytes, the expression of the imprinted gene Kcnq1ot1 decreased significantly [ 98 ]. Our previous study found that after vitrification of bovine MII oocytes, the expression of imprinted genes Peg10, Kcnq1ot1 and Xist in blastocysts obtained by ICSI increased abnormally [ 99 ].…”

Section: Influences Of the Vitrification Technique On Imprinted Genes...mentioning

confidence: 99%

“…Studies have shown that vitrification of mouse oocytes leads to a decrease in the overall DNA methylation level in oocytes and early embryos [ 104 ]. Single-cell whole-genome methylation sequencing (scWGBS) combined with single-cell RNA sequencing (scRNA-seq) also showed that, after the vitrification and warming of mouse MII oocytes, the overall methylation level of the genome decreased abnormally and the expression of the DNA methyltransferase Dnmt1 decreased significantly [ 98 ]. DNMTs expression in vitrified oocytes and the expression of Dnmt3b in blastocysts derived from vitrified oocytes were significantly reduced [ 102 ].…”

Section: Influences Of the Vitrification Technique On Imprinted Genes...mentioning

confidence: 99%

Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes

Chen

Zhang

et al. 2022

Clin Epigenet

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Cryopreservation has important application in assisted reproductive technology (ART). The vitrification technique has been widely used in the cryopreservation of oocytes and embryos, as a large number of clinical results and experimental studies have shown that vitrification can achieve a higher cell survival rate and preimplantation development rate and better pregnancy outcomes. Ovarian tissue vitrification is an alternative method to slow freezing that causes comparatively less damage to the original follicular DNA. At present, sperm preservation mainly adopts slow freezing or rapid freezing (LN2 vapor method), although the vitrification method can achieve higher sperm motility after warming. However, due to the use of high-concentration cryoprotectants and ultra-rapid cooling, vitrification may cause strong stress to gametes, embryos and tissue cells, resulting in potentially adverse effects. Imprinted genes are regulated by epigenetic modifications, including DNA methylation, and show single allele expression. Their accurate regulation and correct expression are very important for the placenta, fetal development and offspring health. Considering that genome imprinting is very sensitive to changes in the external environment, we comprehensively summarized the effect of cryopreservation—especially the vitrification method in ART—on imprinted genes. Animal studies have found that the vitrification of oocytes and embryos can have a significant impact on some imprinted genes and DNA methylation, but the few studies in humans have reported almost no influence, which need to be further explored. This review provides useful information for the safety assessment and further optimization of the current cryopreservation techniques in ART.

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Animal Models for Human Oocyte, Embryo, and Ovarian Tissue Cryopreservation

Leonel,

de Miranda Vasconcellos Vilela,

Amorim

2024

Cryopreservation in Assisted Reproduction

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WGBS combined with RNA-seq analysis revealed that Dnmt1 affects the methylation modification and gene expression changes during mouse oocyte vitrification

Cited by 14 publications

References 58 publications

Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation

Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation

Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes

Animal Models for Human Oocyte, Embryo, and Ovarian Tissue Cryopreservation

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