Wetting transitions within membrane compartments

Guo, Kunkun; Xiao, Wenjia; Yoshikawa, Kenichi

doi:10.1039/c4sm00515e

Cited by 4 publications

(14 citation statements)

References 24 publications

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“…( 2)-( 4) show that the effective contact angles along with the contact line of the three phases are also changed, which will be elucidated in a later work. 30 As expected, if polymer a and b have the same parameters z p , the membrane in contact with phase a is not distinguished from that in contact that with b any more. Thus, the interface between the membrane and a or b would be determined by the interaction energy of polymer a and b within the limited space of the vesicle membrane.…”

Section: Resultssupporting

confidence: 58%

“…With the changes in f a,0 / f b,0 and h bm , the interfaces along the three phases a, b and the membrane are found to be altered as well, and a complete study of the effective contact angles will be presented in a later work. 30 In order to explicitly elucidate the shapes of vesicles encapsulating two phases, morphological phase diagrams are presented as a function of the reduced volume v at varying physical parameters, including the concentration and interaction parameter between the membrane and polymer b. The phase boundaries of vesicles encapsulating two phases between oblates and prolates, and oblates and stomatocytes, are found to move toward higher reduced volumes v, and oblates occupy a much wider range of reduced volumes, compared with 'neat' vesicles.…”

Section: Discussionmentioning

confidence: 99%

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Shapes of vesicles encapsulating two aqueous phases

Xiao

Guo

2014

Soft Matter

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Motivated by recent experiments, vesicles encapsulating two aqueous phases are theoretically explored using a combination of Helfrich curvature elasticity theory for fluid membranes and self-consistent field theory for polymers. The spatial distributions of two polymers, α and β, have been obtained, and two thermodynamic phases occur, as expected. Stable or metastable shapes of fluid and closed vesicles have also been achieved. Due to the impenetrability of the membrane to polymer, the available spaces of polymers α and β are limited and the conformational entropies for the polymers are reduced. Different chain segments that possess different permeability to the membrane would induce different inhomogeneous entropic pressures on the membrane, thereby leading to shape transformations of the vesicles. In the present study, the typical shapes of vesicles encapsulating two phases are studied as functions of the concentrations of polymers α and β, and the interactions between the chain segments and the membrane. The two phases formed by polymers α and β are also found to be altered and have been discussed in detail. In addition, morphological phase diagrams are presented as a function of the reduced volume, v. The phase boundaries between oblates and prolates, and oblates and stomatocytes of vesicles encapsulating two phases are found to move toward the higher reduced volume, and oblates occupy a much wider range of the reduced volume compared with 'neat' vesicles.

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Section: Resultssupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Shapes of vesicles encapsulating two aqueous phases

Xiao

Guo

2014

Soft Matter

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“…2b )[ 36 ]. This parameter, θ in , is a material property (unaffected by factors such as vesicle shape) if spontaneous curvature is negligible[ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Interactions between Phase-Separated Liquids and Membrane Surfaces

Botterbusch

Baumgart

2021

Applied Sciences

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Liquid-liquid phase separation has recently emerged as an important fundamental organizational phenomenon in biological settings. Most studies of biological phase separation have focused on droplets that “condense” from solution above a critical concentration, forming so-called “membraneless organelles” suspended in solution. However, membranes are ubiquitous throughout cells, and many biomolecular condensates interact with membrane surfaces. Such membrane-associated phase-separated systems range from clusters of integral or peripheral membrane proteins in the plane of the membrane to free, spherical droplets wetting membrane surfaces to droplets containing small lipid vesicles. In this review, we consider phase-separated liquids that interact with membrane surfaces and we discuss the consequences of those interactions. The physical properties of distinct liquid phases in contact with bilayers can reshape the membrane, and liquid-liquid phase separation can construct membrane-associated protein structures, modulate their function, and organize collections of lipid vesicles dynamically. We summarize the common phenomena that arise in these systems of liquid phases and membranes.

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“…Ma et al (10) and Choi et al (11) confined aqueous two-phase systems (ATPSs) in microdroplets and fabricated microgels by selective polymerization. In such a confined space, phase separation accompanies wetting of a polymer to the substrate (12)(13)(14)(15). Although the selective polymerization of phase-separated polymers in microdroplets has a great potential to produce variously shaped microgels, the dynamical coupling among the phase separation, wetting, and gelation of polymers in a confined space remains unclear (16).…”

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confidence: 99%

Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation

Yanagisawa

Nigorikawa

Sakaue

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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We report the spontaneous patterning of polymer microgels by confining a polymer blend within microspheres. A poly(ethylene glycol) (PEG) and gelatin solution was confined inside water-in-oil (W/O) microdroplets coated with a layer of zwitterionic lipids: dioleoylphosphatidylethanolamine (PE) and dioleoylphosphatidylcholine (PC). The droplet confinement affected the kinetics of the phase separation, wetting, and gelation after a temperature quench, which determined the final microgel pattern. The gelatin-rich phase completely wetted to the PE membrane and formed a hollow microcapsule as a stable state in the PE droplets. Gelation during phase separation varied the relation between the droplet size and thickness of the capsule wall. In the case of the PC droplets, phase separation was completed only for the smaller droplets, wherein the microgel partially wetted the PC membrane and had a hemisphere shape. In addition, the temperature decrease below the gelation point increased the interfacial tension between the PEG/ gelatin phases and triggered a dewetting transition. Interestingly, the accompanying shape deformation to minimize the interfacial area was only observed for the smaller PC droplets. The critical size decreased as the gelatin concentration increased, indicating the role of the gel elasticity as an inhibitor of the deformation. Furthermore, variously patterned microgels with spherically asymmetric shapes, such as discs and stars, were produced as kinetically trapped states by regulating the incubation time, polymer composition, and droplet size. These findings demonstrate a way to regulate the complex shapes of microgels using the interplay among phase separation, wetting, and gelation of confined polymer blends in microdroplets. microgels | aqueous two-phase systems | sol-gel phase separation | hydrogels | emulsions T he regulation of the 3D shapes of biopolymer gels at the mesoscale has numerous applications in the biomedical, cosmetic, and food materials industries, among others (1). Recently, top-down and bottom-up approaches have been reported to control the mesoscopic patterns of polymer gels. For example, photolithography and two-photon polymerization allow the regulation of gel patterns at the mesoscale (2-4). The advanced design of the molecules enables polymerization with a self-assembly and produces nonspherical microgels: spherical particles with a cavity, capsules, and cubic particles (5-7). However, these methods require highly specialized equipment and are generally difficult to adapt for biopolymer gels.Dynamical coupling between phase separation and sol-gel transition in polymer blends has also been investigated for the spontaneous formation of spherical microgels and a porous gel (8, 9). Ma et al. (10) and Choi et al. (11) confined aqueous two-phase systems (ATPSs) in microdroplets and fabricated microgels by selective polymerization. In such a confined space, phase separation accompanies wetting of a polymer to the substrate (12-15). Although the selective polymerization of phase-se...

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Wetting transitions within membrane compartments

Cited by 4 publications

References 24 publications

Shapes of vesicles encapsulating two aqueous phases

Shapes of vesicles encapsulating two aqueous phases

Interactions between Phase-Separated Liquids and Membrane Surfaces

Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation

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