Wet adhesive hydrogels to correct malacic trachea (tracheomalacia) A proof of concept

Uslu, Ece; Rana, Vijay Kumar; Anagnostopoulos, Sokratis; Karami, Peyman; Bergadano, Alessandra; Courbon, Cecile; Gorostidi, François; Sandu, Kishore; Stergiopulos, Nikolaos; Pioletti, Dominique P.

doi:10.1016/j.isci.2023.107168

Cited by 2 publications

(10 citation statements)

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“…The presence of this surface lubrication can affect the adhesion strength of the hydrogel. , That is why we observed lower adhesion values on these tissues with both hydrogel formulations. The adhesion performance of the developed hydrogels was also compared to a commercially available tissue adhesive called TISSEEL(Baxter), specifically on rabbit trachea surface . The results showed that w/o PEG-NHS hydrogel and w/ PEG-NHS hydrogel exhibited six-times and nine-times higher shear adhesion strength than TISSEEL, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, stent materials are too rigid to accommodate the trachea’s natural movement during breathing, hindering the tissue growth . To address these challenges, we proposed a new approach in our recent study: using an adhesive hydrogel patch to support the weakened trachea and prevent collapse . To this end, we performed ex vivo studies with rabbit tracheas (consisting of a 10 cm long trachea and the larynx) to examine the potential use of the HEAam-based adhesive hydrogels for tracheomalacia treatment.…”

Section: Resultsmentioning

confidence: 99%

“…HEAam-based hydrogel without PEG-NHS was developed using a method published earlier by our group. 12 In brief, HEAam (20−50% w/v), PEGDMA (1 to 3% w/v), and photoinitiator (PI) LAP (0.05% w/v) were dissolved in PBS using vortex in the absence of light. The precursor solution was poured into a 15 × 15 × 0.7 mm 3 custom-made Teflon molds and covered with plastic slides.…”

Section: ■ Experimental Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Recently, we proposed a series of adhesive hydrogels based upon hydroxyethyl acrylamide (HEAam) and showed their potential use to correct tracheomalacia in a proof-of-concept study. 12 These hydrogels exhibited excellent adhesive properties on the rabbit trachea surface. However, these hydrogels lack durability under wet conditions, a common problem in adhesive hydrogels, which results in deterioration in both adhesive strength and mechanical characteristics over time.…”

Section: ■ Introductionmentioning

confidence: 99%

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Enhancing Robustness of Adhesive Hydrogels through PEG-NHS Incorporation

Uslu,

Rana,

Guo

et al. 2023

ACS Appl. Mater. Interfaces

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Tissue wounds are a significant challenge for the healthcare system, affecting millions globally. Current methods like suturing and stapling have limitations as they inadequately cover the wound, fail to prevent fluid leakage, and increase the risk of infection. Effective solutions for diverse wound conditions are still lacking. Adhesive hydrogels, on the other hand, can be a potential alternative for wound care. They offer benefits such as firm sealing without leakage, easy and rapid application, and the provision of mechanical support and flexibility. However, the in vivo durability of hydrogels is often compromised by excessive swelling and unforeseen degradation, which limits their widespread use. In this study, we addressed the durability issues of the adhesive hydrogels by incorporating acrylamide polyethylene glycol N-hydroxysuccinimide (PEG-NHS) moieties (max. 2 wt %) into hydrogels based on hydroxy ethyl acrylamide (HEAam). The results showed that the addition of PEG-NHS significantly enhanced the adhesion performance, achieving up to 2-fold improvement on various soft tissues including skin, trachea, heart, lung, liver, and kidney. We further observed that the addition of PEG-NHS into the adhesive hydrogel network improved their intrinsic mechanical properties. The tensile modulus of these hydrogels increased up to 5-fold, while the swelling ratio decreased up to 2-fold in various media. These hydrogels also exhibited improved durability under the enzymatic and oxidative biodegradation induced conditions without causing any toxicity to the cells. To evaluate its potential for clinical applications, we used PEG-NHS based hydrogels to address tracheomalacia, a condition characterized by inadequate mechanical support of the airway due to weak/malacic cartilage rings. Ex vivo study confirmed that the addition of PEG-NHS to the hydrogel network prevented approximately 90% of airway collapse compared to the case without PEG-NHS. Overall, this study offers a promising approach to enhance the durability of adhesive hydrogels by the addition of PEG-NHS, thereby improving their overall performances for various biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Experimental Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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