Western blotting is useful in the salivary diagnosis ofHelicobacter pyloriinfection

Abstract

Background-The salivary diagnosis ofHelicobacter pylori infection oVers attractive possibilities for the epidemiological study of infection in children. Salivary enzyme linked immunosorbent assay (ELISA) is less reliable then serum ELISA, owing to variable transudation of immunoglobulin. In addition, children are more diYcult to study because of lower specific serum antibody concentrations to H pylori. The performance of salivary western blotting in comparison with serum western blotting and serum ELISA was investigated in school children. Subjects and methods-Paired serum and saliva specimens were obtained from 665 school children aged 9-11 in 10 British towns. All saliva and serum specimens were first analysed by ELISA; subsequently, western blotting of both specimens was performed on 31 and 34 specimens, respectively, to establish the criteria for positivity for western blotting. The remaining 121 specimens were then tested blindly and saliva was compared with the serum. Results-The sensitivity and specificity of salivary ELISA in the 665 specimens was 32 of 50 (64%) and 530 of 691 (87%), respectively, when compared with serum ELISA. The western blotting validation was performed on 28 subjects with positive serum and positive salivary ELISA, 28 saliva positives with negative serum, 16 saliva negatives with positive serum, and 50 doubly negative subjects. Compared with serum western blots, the sensitivity and specificity of salivary western blots was 38 of 47 (81%) and 68 of 75 (91%), respectively. Using serum ELISA as the gold standard, the sensitivity and specificity were 32 of 44 (73%) and 72 of 78 (92%), respectively, the specificity being significantly higher than salivary ELISA (p < 0.001). Conclusion-Salivary western blotting forIgG is useful in the diagnosis of H pylori infection and is superior to ELISA. It also permits the identification of pathogenic strains. (J Clin Pathol 2000;53:314-317)

Diagnosis ofHelicobacter pyloriInfection in Children: Comparison of a Salivary Immunoglobulin G Antibody Test with the [13C]Urea Breath Test

“…Data obtained from children referred for endoscopy show comparable sensitivity (93%) and specificity (82%) when tested against histology and the urease test (10). Two studies investigated asymptomatic children by using either a serology-based test (sensitivity, 64%; specificity, 87%) or the [ 13 C]UBT (sensitivity, 65%; specificity, 95%) (1,12). The results obtained with the HM-CAP ELISA kit in this study for asymptomatic children (sensitivity, 81%; specificity, 95%) compared to the [ 13 C]UBT are promising.…”

“…Noninvasive diagnosis of H. pylori infection can be done by measuring specific anti-H. pylori immunoglobulin G (IgG) antibodies in serum or saliva with an enzyme-linked immunosorbent assay (IgG-ELISA), with the [ 13 C]urea breath test ([ 13 C]UBT), and with an enzyme immunoassay (HpSA) for antigens in stools (3, 12-14, 16-18, 21-25). Whole saliva as a test sample is easily accessible, and despite some less encouraging results with H. pylori testing (9,19,23), some studies seem to be promising (1,6,10,11,15).…”

Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009

“…Six papers evaluated the presence of antibodies in oral specimens using a variety of commercially available kits and collection devices [3,5,38,41,77,78,107]. At least two of the studies sampled asymptomatic preschool children (in day-care or random samples) and used UBT as the reference standard [77,78].…”

Valor Diagnóstico da Saliva em Doenças Orais e Sistêmicas: Uma Revisão de Literatura

“…É sabido que os métodos invasivos demandam altos custos e que os métodos não invasivos tais como os exames sorológicos do sangue e o teste da respiração da uréia estão se tornando mais popular. O método PCR provou ser altamente sensível e específico e é considerado como o método da escolha para detectar o DNA do Helicobacter pylori na boca (BALLAM et al, 2000;BERROTERÁN et al, 2002).…”