Western Blot Analysis of Antibodies to Leishmania infantum Antigens: Potential of the 14-KD and 16-KD Antigens for Diagnosis and Epidemiologic Purposes

Mary, Charles; Lamouroux, D; Dunan, S.; Quilici, M

doi:10.4269/ajtmh.1992.47.764

Cited by 124 publications

(112 citation statements)

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“…Sera from healthy individuals were analyzed by enzyme-linked immunosorbent assay (ELISA) using a lysate of Leishmania infantum as antigen and immunoblotting as previously described. 3 Immunoblots were scored as positive when antibodies to the 14-kD and/or 16-kD antigens of Leishmania were present.…”

Section: Methodsmentioning

confidence: 99%

“…The ability of in vitro assays to detect such silent infections differs among the different disease-endemic areas for VL and the technical means used. 3,4 Increased sensitivity for Leishmania detection has been achieved by means of the polymerase chain reaction (PCR), notably by targeting kinetoplast DNA. Analysis of the results obtained with different assays indicates that in the Mediterranean area the parasitic load in asymptomatic carriers is lower than that observed when the disease is diagnosed.…”

Section: Introductionmentioning

confidence: 99%

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Reference Values for Leishmania Infantum Parasitemia in Different Clinical Presentations: Quantitative Polymerase Chain Reaction for Therapeutic Monitoring and Patient Follow-Up

Mary

Faraut²,

Drogoul³

et al. 2006

The American Journal of Tropical Medicine and Hygiene

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Abstract. Quantification of Leishmania infantum DNA in blood samples by an ultrasensitive quantitative polymerase chain reaction (QPCR) detected parasitemias in different clinical presentations. We observed a large range of parasitemias, more than 9 log values, and could determine the threshold between asymptomatic carriage and disease in the Mediterranean area (approximately one parasite/mL of blood. Based on kinetoplast DNA amplification, this assay had a sensitivity of 0.001 parasite DNA equivalents/mL and detected asymptomatic carriage of Leishmania. It detected parasite DNA in 58% of healthy subjects, while an immunoblot detected specific antibodies in only 16%. For initial diagnosis of disease, this quantitative PCR with blood samples constitutes a non-invasive alternative to bone marrow aspiration. Its main applications are monitoring of drug therapy and follow-up of immunodeficient patients for biologic confirmation of relapses.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reference Values for Leishmania Infantum Parasitemia in Different Clinical Presentations: Quantitative Polymerase Chain Reaction for Therapeutic Monitoring and Patient Follow-Up

Mary

Faraut²,

Drogoul³

et al. 2006

The American Journal of Tropical Medicine and Hygiene

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“…The western blot technique provides detailed antibody responses to various leishmanial antigens [69,70], and has been found to be more sensitive than the IFA test and ELISA, especially in co-infected HIV patients with VL [71][72][73], but the drawbacks of the technique (equipment and time requirements, cumbersomeness, and cost) limit its use to research laboratories.…”

Section: Immunoblotting (Western Blot)mentioning

confidence: 99%

Diagnosis of leishmaniasis

Elmahallawy

Martínez

Rodríguez‐Grangér

et al. 2014

J Infect Dev Ctries

152

119

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Leishmaniasis is a clinically heterogeneous syndrome caused by intracellular protozoan parasites of the genus Leishmania. The clinical spectrum of leishmaniasis encompasses subclinical ( not apparent), localized (skin lesion), and disseminated (cutaneous, mucocutaneous, and visceral) infection. This spectrum of manifestations depends on the immune status of the host, on the parasite, and on immunoinflammatory responses. Visceral leishmaniasis causes high morbidity and mortality in the developing world. Reliable laboratory methods become mandatory for accurate diagnosis, especially in immunocompromised patients such as those infected with HIV. In this article, we review the current state of the diagnostic tools for leishmaniasis, especially the serological test.

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“…This percentage is inversely proportional to CD4 cell depletion. Anti-Leishmania antibodies in HIV-positive patients are 50 times less than those in HIV-negative patients 91 . Therefore, there may be many false negative tests.…”

mentioning

confidence: 85%

Repair of Impaired Host Peroxisomal Properties Cropped Up Due to Visceral Leishmaniasis May Lead to Overcome Peroxisome Related Genetic Disorder Which May Develop Later After Treatment

Datta¹,

Gupta²,

Raychaudhury³

2011

Advances in the Study of Genetic Disorders

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Western Blot Analysis of Antibodies to Leishmania infantum Antigens: Potential of the 14-KD and 16-KD Antigens for Diagnosis and Epidemiologic Purposes

Cited by 124 publications

References 0 publications

Reference Values for Leishmania Infantum Parasitemia in Different Clinical Presentations: Quantitative Polymerase Chain Reaction for Therapeutic Monitoring and Patient Follow-Up

Reference Values for Leishmania Infantum Parasitemia in Different Clinical Presentations: Quantitative Polymerase Chain Reaction for Therapeutic Monitoring and Patient Follow-Up

Diagnosis of leishmaniasis

Repair of Impaired Host Peroxisomal Properties Cropped Up Due to Visceral Leishmaniasis May Lead to Overcome Peroxisome Related Genetic Disorder Which May Develop Later After Treatment

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