2017
DOI: 10.1128/jvi.02396-16
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West Nile Virus NS1 Antagonizes Interferon Beta Production by Targeting RIG-I and MDA5

Abstract: West Nile virus (WNV) is a mosquito-borne flavivirus that causes epidemics of encephalitis and viscerotropic disease worldwide. This virus has spread rapidly and has posed a significant public health threat since the outbreak in New York City in 1999. The interferon (IFN)-mediated antiviral response represents an important component of virus-host interactions and plays an essential role in regulating viral replication. Previous studies have suggested that multifunctional nonstructural proteins encoded by flavi… Show more

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Cited by 66 publications
(67 citation statements)
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References 61 publications
(83 reference statements)
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“…During virus invasion, multiple signaling pathways can be activated to facilitate the high expression of type I IFN and downstream antiviral genes, mainly including RLRs and TLRs (29,30). Previously, it has been extensively shown that flaviviruses, such as dengue virus (DENV), hepatitis C virus, and West Nile virus, can target the RLR pathway, thus blocking the key processes of type I IFN signal transduction and antagonizing the innate immunity with different molecular mechanisms (22,23,31). To explore the potential correlation between NS3/NS2B3-mediated IFNb antagonism and RLR signaling pathway, we constructed Flag-tagged RIG-I-2CARD, MAVS, MITA, TBK1, IRF3-5D expression plasmids and then cotransfected them with HA-tagged NS3 or NS2B3 into HEK293T cells.…”
Section: Zikv Ns3 and Ns2b3 Attenuate Ifn-b Expressionmentioning
confidence: 99%
“…During virus invasion, multiple signaling pathways can be activated to facilitate the high expression of type I IFN and downstream antiviral genes, mainly including RLRs and TLRs (29,30). Previously, it has been extensively shown that flaviviruses, such as dengue virus (DENV), hepatitis C virus, and West Nile virus, can target the RLR pathway, thus blocking the key processes of type I IFN signal transduction and antagonizing the innate immunity with different molecular mechanisms (22,23,31). To explore the potential correlation between NS3/NS2B3-mediated IFNb antagonism and RLR signaling pathway, we constructed Flag-tagged RIG-I-2CARD, MAVS, MITA, TBK1, IRF3-5D expression plasmids and then cotransfected them with HA-tagged NS3 or NS2B3 into HEK293T cells.…”
Section: Zikv Ns3 and Ns2b3 Attenuate Ifn-b Expressionmentioning
confidence: 99%
“…Many viruses have developed different mechanisms to escape the antiviral response, including counteracting the activity of ISG15 (Guerra, Cáceres, Knobeloch, Horak, & Esteban, ; Yuan & Krug, ). Several studies have addressed NNV persistent infections in many fish, indicating NNV has developed some mechanisms to antagonize host innate antiviral responses (Lu et al, ; Zhang et al, ). We speculated that RGNNV might counteract the antiviral activity of ISG15 to escape the host antiviral response in LJB cells.…”
Section: Discussionmentioning
confidence: 99%
“…In Figs. 3 and 4, the HEK293 T cells were seeded in 24-well plates and transfected with the Flag-nsp16, Myc-nsp10, IFN-β-luc reporter, the LacZ and poly I:C or Flag-RIG-I(N) or Flag-MDA5(N) (Zhang et al, 2017) for 24 h and then the cells were lysed for luciferase assays and western blotting.…”
Section: Luciferase Assaymentioning
confidence: 99%