West Nile Virus

doi:10.1159/000353698

Cited by 17 publications

(9 citation statements)

References 172 publications

(251 reference statements)

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“…The intensified surveillance for WNV infections in humans and animals resulted in the detection and isolation of many pathogenic isolates of WNV, classified in at least seven different lineages [2,3]. However, two major WNV lineages, lineage 1 (L1) and lineage 2 (L2), are responsible for the outbreaks observed in the past years.…”

Section: Wnv Virology and Clinical Perspectivementioning

confidence: 99%

West Nile Virus in Europe and Safety of Blood Transfusion

Pisani

Cristiano

Pupella

et al. 2016

Transfus Med Hemother

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West Nile virus (WNV) has become an increasing issue in the transfusion setting since 2002, when it was firstly shown in the USA that it can be transmitted through blood transfusion. Since then, several precautionary measures have been introduced in Europe in order to reduce the possible risk of transmission via transfusion/solid organ transplantation. In addition, the epidemiological surveillance has been tightened and the network for communication of human WNV cases strengthened. This review will focus on WNV circulation and the safety of blood in Europe.

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Section: Wnv Virology and Clinical Perspectivementioning

confidence: 99%

West Nile Virus in Europe and Safety of Blood Transfusion

Pisani

Cristiano

Pupella

et al. 2016

Transfus Med Hemother

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“…Durch Arthropoden übertragbare Viren (Arboviren) und insbesondere Viren aus dem Genus Flavivirus der Flaviviridae haben sich in den vergangenen Jahrzehnten in vielen subtropischen und tropischen Ländern ausgebreitet [1]. Diese schließen Dengueviren (DENV), Chikungunyavirus (CHIKV), West-Nil-Virus (WNV) und Usutuvirus (USUV) ein [1][2][3][4]. Für DENV und CHIKV bilden der Mensch und andere Primaten das Virusreservoir.…”

Section: Wissensstand üBer Den Erregerunclassified

“…Aus diesen und weiteren Befunden, die bei Ausbrüchen gewonnen wurden, lässt sich ableiten, dass die Inkubationszeit etwa zwei bis sieben Tage beträgt und damit vergleichbar zum Infektionsverlauf anderer Flavivirus-Infektionen wie Dengue oder West-Nil-Fieber ist [2,3,5,41,42]. Man geht davon aus, dass etwa 80 % der Personen, die sich mit ZIKV infizieren, keine Symptome entwickeln.…”

Section: Infektion Und Infektionskrankheitunclassified

Zikavirus (ZIKV)

2016

Bundesgesundheitsbl

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“…In humans, most of WNV infections remain asymptomatic, but approximately 20% of the individuals develop WNF. In a small percentage (<1%) of those infected, severe outcome with neurological symptoms occurs [2]. In contrast, in the USA West Nile neuro-invasive disease (WNND) is described in 61% of reported WNV disease cases in the year 2014 [3].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, in the USA West Nile neuro-invasive disease (WNND) is described in 61% of reported WNV disease cases in the year 2014 [3]. This led to a reassessment of previous WNV outbreaks in Europe and an intensification of the epidemiological surveillance [2]. An increasing number of WNV infections in humans in the Mediterranean Basin (Israel, Italy, Greece, and North Africa), Romania, Hungary, Austria, Russia, and several Central Asian countries has been reported in recent years ( www.ECDC.europa.eu ; [4,5]).…”

Section: Introductionmentioning

confidence: 99%

Implementation of NAT Screening for West Nile Virus and Experience with Seasonal Testing in Germany

Dreier

Vollmer

Hinse

et al. 2015

Transfus Med Hemother

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Background: West Nile virus (WNV) can be transmitted by transfusion through infected blood components. In Germany, a 28-day deferral for blood donors of therapeutic blood components who had spent at least 2 days in WNV-endemic areas from June 1 to November 30, 2014 was enforced. Otherwise, screening of blood donors for WNV RNA or the application of pathogen reduction techniques are appropriate alternatives. Methods: In the present study, we evaluated NAT screening for the detection of WNV in blood components. A total of 58 minipools consisting of 357 individual blood donors were screened for the presence of WNV RNA employing an automated high-volume extraction method using the RealStar WNV RT-PCR Kit. Additionally, different WNV reference reagents were quantified to prove the status quo of standardization. Four different WNV real-time NAT kits were compared using samples of an external quality assessment panel. Results: The 95% lower detection limit of the WNV MP-NAT was determined to 30.2 copies/ml (95% CI 24.2-45.4 copies/ml). No WNV RNA-positive minipool was detected. Quantification of WNV reference reagents revealed shortcomings in standardization. Comparison of several WNV NAT assays showed considerable differences in assay sensitivities and particularly a missing detection of WNV lineage 2. Implementation of seasonal WNV MP-NAT screening was demonstrated. Conclusion: Actually, WNV infections in Germany are rare events introduced by returning travelers, but surveillance of these emerging infections is important for safety in blood supply. The validation study pointed out the need for standardization of WNV NAT because of current lack of an international standard for WNV RNA.

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West Nile Virus

Cited by 17 publications

References 172 publications

West Nile Virus in Europe and Safety of Blood Transfusion

West Nile Virus in Europe and Safety of Blood Transfusion

Zikavirus (ZIKV)

Implementation of NAT Screening for West Nile Virus and Experience with Seasonal Testing in Germany

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