Wen-pi-tang-Hab-Wu-ling-san attenuates kidney ischemia/reperfusion injury in mice

Expression and distribution of heat shock proteins in the heart of transported pigs

“…Several studies in animal models have documented the cytoprotective activity of Hsps against ischemia and reperfusion-associated damage in the heart (Wu and Tanguay 2006;Seok et al 2007). By immunohistochemical analysis in the endothelial cells and in the wall of the heart blood vessels, Hsp70 and Hsp27 were intracytoplasmically detected.…”

“…Several studies have shown that earlier experience with the stressor helps to improve induction (Donnelly et al 1992;Marber et al 1993;Yellon et al 1992;Walker et al 1993;van Ginneken et al 2006), transfection (Suzuki et al 1997), or overexpression of Hsp proteins if the stressor occurs again (Marber et al 1995;Ooie et al 2005;Gauthaman et al 2005;Chiu et al 2003). It is known that Hsp induction, and particularly Hsp70, can protect the myocardium from ischemia and reduces the infarction size in mice (Wu and Tanguay 2006;Seok et al 2007). Our immunoblot results show that all five Hsps are detected in the control pigs.…”

Increased superoxide formation induced by irradiation preconditioning triggers kidney resistance to ischemia-reperfusion injury in mice

“…Histological damage levels were scored as described previously (14,39). Briefly, 50 tubules in the outer medulla of the kidney were analyzed by using a score as follows: 0, no damage; 1, mild damage with rounding of epithelial cells and dilated tubular lumen; 2, severe damage with flattened epithelial cells, loss of nuclear staining, dilated lumen, and congestion of lumen; and 3, destroyed tubules with flat epithelial cells lacking nuclear staining and congestion of lumen.…”

A review of oxidative stress in acute kidney injury: protective role of medicinal plants-derived antioxidants

“…Like ROS/RNS, resulting lipid peroxides are dangerous to cells due to its ability to sustain free radical cascades, eventually leading to increased membrane rigidity and abnormalities of endothelial function which may be involved in the pathophysiology of AKI (Pieper et al, 1997;Farooqui & Horrocks 1998). Moreover, OS-related AKI was significantly reduced by endogenous antioxidants (Seok et al, 2007;Ghule et al, 2011). GR-deficient mice were used to demonstrate ROS-mediated renal proximal tubule injury (Rogers et al, 2006).…”