Well-defined polyester-grafted silica nanoparticles for biomedical applications: Synthesis and quantitative characterization

Abstract

Polyester-based composites with silica nanoparticles fillers are promising candidates as biomaterials due to improved mechanical and biological properties. However, nanofillers use generally leads to an inhomogeneous distribution inside the polymer matrix because of agglomeration, decreasing composites overall performances. In view of improving nanofillers dispersion, we developed a synthesis and characterization method to design poly(D, L-lactide)-grafted silica nanoparticles using "grafting to" method and to quantify the amount of grafted poly(D,Llactide). Firstly, well-defined N-hydroxysuccinimide ester poly(D,L-lactide)s were synthesized through a new pathway. Then, amino-functionalized silica nanoparticles were grafted with those customized polyesters yielding an amide covalent bond between both reagents. Such PDLLA-grafted nanoparticles were precisely characterized and the grafting amount was quantified using a dual approach based on TGA and FTIR analysis. The synthesis and the characterization methods developed constitute a robust and reproducible way to design well-defined polymer-grafted silica nanoparticles that could be used as nanofillers in polymer matrix nanocomposites for biomedical applications.

Poly(d,l-lactide)-Grafted Bioactive Glass Nanoparticles: From Nanobricks to Freeze-Cast Scaffolds for Bone Substitution

“…Two different kinds of polymers were synthesized. N -Hydroxysuccinimide ester poly­( d,l -lactide) (PDLLA–COO–NHS, PDLLA_1 ), used for PDLLA-grafted BGNp synthesis, was obtained by postpolymerization modification of poly­( d,l -lactide) following a protocol (Scheme S1) already described in a previous work . High-molecular-weight poly­( d,l -lactide), PDLLA_2 , added to the nanocomposite suspension before a freeze-casting process, was prepared by ring-opening polymerization (ROP) in bulk.…”

“…PDLLA-grafted bioglass nanoparticles ( BGNp-PDLLA_1 ) were synthesized by adapting a grafting protocol described in previous work . Briefly, amino-functionalized bioglass nanoparticles were obtained by a reaction with 3-aminopropyl triethoxysilane (APTES) in anhydrous toluene under an inert atmosphere.…”

Analyzing the surface of functional nanomaterials—how to quantify the total and derivatizable number of functional groups and ligands

“…For example, TGA-MS has been used to determine the amount of aromatic molecules adsorbed on CNT [251] and to identify the organic compounds extractable from various NM [252]. Moreover, TGA has been used in multi-method approaches for quantitative analysis to enhance the reliability of the results by comparing them with information from FTIR, NMR, ICP-MS/OES, and XPS measurements [164,175,186,[253][254][255][256]. And just recently, TGA has been applied to quantify adsorbed citrate molecules on the surface of gold NP, which provided new insights into mechanistic details of the well-known Turkevich gold NP synthesis method [257].…”

Mesoporous silica nanoparticles, methods of preparation and use of bone tissue engineering

“…Silicates stimulate type I collagen formation and osteoblastic differentiation. Silicates are needed for the formation and calcification of bone tissue in metabolic processes [69]. In bone, it links to glycosaminoglycans and it is needed to form between collagen and proteoglycans.…”

“…Therefore, those with unique surface properties and porosity emerged as bioactive materials for bone regeneration [42]. It is also known that the silicate used as an additive improves the mechanical properties and structure of the final product [69].…”