Well-defined nanostructured surface-imprinted polymers for the highly selective enrichment of low-abundance protein in mammalian cell extract

Tang, Ya-Ting; Yao, Yanhuan; Yang, Xingxing; Zhu, Ting; Huang, Yapeng; Chen, Haiyang; Wang, Ying; Mi, Huaifeng

doi:10.1039/c6nj01500j

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…For instance, Fan and coworkers have described a peptide extraction approach based on nanoporous silica thin films to successfully detect low concentrations of hepcidin from human body fluids collected from 119 healthy volunteers and 19 inflammation patients [55]. Recently, Tang and coworkers reported an approach for the adsorption and enrichment of low-abundance protein in mammalian cell extract by using nanostructured surface-imprinted polymers with sufficiently thin shells and high surface-to-volume ratios [56].…”

Section: Enrichment Of Low-abundance Lmw Proteins Basedmentioning

confidence: 99%

Strategies for Characterization of Low-Abundant Intact or Truncated Low-Molecular-Weight Proteins From Human Plasma

Cai

Yang

2017

The Enzymes

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Low-molecular-weight region (LMW, MW≤30kDa) of human serum/plasma proteins, including small intact proteins, truncated fragments of larger proteins, along with some other small components, has been associated with the ongoing physiological and pathological events, and thereby represent a treasure trove of diagnostic molecules. Great progress in the mining of novel biomarkers from this diagnostic treasure trove for disease diagnosis and health monitoring has been achieved based on serum samples from healthy individuals and patients and powerful new approaches in biochemistry and systems biology. However, cumulative evidence indicates that many potential LMW protein biomarkers might still have escaped from detection due to their low abundance, the dynamic complexity of serum/plasma, and the limited efficiency of characterization approaches. Here, we provide an overview of the current state of knowledge with respect to strategies for the characterization of low-abundant LMW proteins (small intact or truncated proteins) from human serum/plasma, involving prefractionation or enrichment methods to reduce dynamic range and mass spectrometry-based characterization of low-abundant LMW proteins.

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Section: Enrichment Of Low-abundance Lmw Proteins Basedmentioning

confidence: 99%

Strategies for Characterization of Low-Abundant Intact or Truncated Low-Molecular-Weight Proteins From Human Plasma

Cai

Yang

2017

The Enzymes

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Novel synthetic method for tubular hypercrosslinked polymer nanofibers and its mechanism

Wang¹,

Fan²,

Zhang³

2023

Fabrication and Functionalization of Advanced Tubular Nanofibers and Their Applications

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Synthesis of surface imprinted polymers based on wrinkled flower-like magnetic graphene microspheres with favorable recognition ability for BSA

Yang

Cui

et al. 2021

Journal of Materials Science & Technology

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Well-defined nanostructured surface-imprinted polymers for the highly selective enrichment of low-abundance protein in mammalian cell extract

Abstract: A new approach for the adsorption and enrichment of natural low-abundance protein by using nanostructured surface-imprinted polymers is presented.

Cited by 3 publications

References 42 publications

Strategies for Characterization of Low-Abundant Intact or Truncated Low-Molecular-Weight Proteins From Human Plasma

Strategies for Characterization of Low-Abundant Intact or Truncated Low-Molecular-Weight Proteins From Human Plasma

Novel synthetic method for tubular hypercrosslinked polymer nanofibers and its mechanism

Synthesis of surface imprinted polymers based on wrinkled flower-like magnetic graphene microspheres with favorable recognition ability for BSA

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