“…The differentiation of infection structures on water or on a range of artificial media is promoted by Zn++ (15,56,58), by cystein and glutathione (32,33), by unidentified, volatile stimulants (2, 30), by pelargonaldehyde (31), by exposure to the proper conditions of light and temperature (24,46) and by contact with artificial membranes (19,20,46). Of these, treatment with ALLEN'S stimulant and the manipulation of light and temperature are by far the most effective.…”