Weissella koreensis and Pediococcus pentosaceus bacterial ghosts induce inflammatory responses as immunostimulants

Lee, Jieun; Lee, Kwang-Su; Lee, Junwon; Lee, Ki-Sung; Park, Shin‐Young

doi:10.1016/j.bbrc.2023.07.049

Cited by 2 publications

(3 citation statements)

References 26 publications

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“…P. pentosaceus CACC616 showed excellent probiotic properties compared to the LGG control, as well as bacterial safety for using the probiotics such as the producing ability of biogenic amines, antibiotic resistance, and hemolytic ability. Lee et al (2023) observed the immune modulation effects of P. pentosaceus as immunostimulants, elevating the mRNA levels of cytokines IL-10, IL-6, and IL-1β [35,36]. These studies corroborate our findings; therefore, P. pentosaceus CACC616 can be applied as a potential probiotic.…”

Section: Discussionsupporting

confidence: 88%

“…pentosaceus-supplemented diets significantly improved the growth performance of pigs and reduced fecal noxious gas and diarrhea incidence [14,17]. P. pentosaceus can modulate immunity and ameliorate gut inflammation by maintaining gut epithelial integrity [36,37]. Our results corroborated these findings, as fecal calprotectin, an indicator of intestinal inflammation, was significantly reduced in the CACC616 group.…”

Section: Discussionsupporting

confidence: 81%

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Assessing the Probiotic Effects of Pediococcus pentosaceus CACC616 in Weaned Piglets

Park,

Song,

Park

et al. 2023

Microorganisms

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During weaning, piglets experience various stressor events that disrupt their gut microbiota and immune balance, decrease growth parameters, and increase mortality rates. In this study, we assessed the efficacy of Pediococcus pentosaceus CACC616 as a probiotic supplement. We characterized this strain and evaluated its effect on improving growth performance, modulating gut microbiota composition, and reducing noxious odor components in weaned piglets compared to a non-supplementary diet (control). During the 26-day period, 40 crossbred weaned piglets were randomly assigned to pens with 20 animals each in two groups: control and treatment groups with CACC616. On day 26, the treatment group exhibited a lower feed conversion ratio (FCR) and a significant alteration in gut microbial composition, correlating with improved growth parameters and gut health (p < 0.05). The treatment group also exhibited significantly reduced digestibility- and intestinal-environment-related noxious odor components (p < 0.05). The CACC616 strain effectively reduced pathogenic genera numbers, including Campylobacter, Mogibacterium, Escherichia–Shigella, and Desulfovibrio spp., with the treatment group exhibiting lower fecal calprotectin levels than the control group (p < 0.05). Overall, this study revealed that the functional probiotic CACC616 contributes to enhanced FCR and effectively modulates weaned piglets’ inflammation and intestinal microbiota.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 81%

Assessing the Probiotic Effects of Pediococcus pentosaceus CACC616 in Weaned Piglets

Park,

Song,

Park

et al. 2023

Microorganisms

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“…Renowned for their high load-bearing capacity, composite preservation, stability at room temperature, scalability, and adaptability, BGs find application across diverse domains. They serve as promising vaccine candidates [3][4][5], as drug delivery carriers [6,7], to present antigens [8], and as immune stimulants to induce inflammatory response [9]. Moreover, BGs possess innate immunostimulatory properties, eliminating the need for additional adjuvants and holding potential as natural adjuvants for future vaccine formulations and cancer treatments [10,11].…”

Section: Introductionmentioning

confidence: 99%

Construction and Mechanism Exploration of Highly Efficient System for Bacterial Ghosts Preparation Based on Engineered Phage ID52 Lysis Protein E

Ma,

Wang,

Hong

et al. 2024

Vaccines

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Bacterial ghosts (BGs) are hollow bacterial cell envelopes with intact cellular structures, presenting as promising candidates for various biotechnological and biomedical applications. However, the yield and productivity of BGs have encountered limitations, hindering their large-scale preparation and multi-faceted applications of BGs. Further optimization of BGs is needed for the commercial application of BG technology. In this study, we screened out the most effective lysis protein ID52-E-W4A among 13 mutants based on phage ID52 lysis protein E and optimized the liquid culture medium for preparing Escherichia coli Nissle 1917 (EcN). The results revealed a significantly higher lysis rate of ID52-E-W4A compared to that of ID52-E in the 2xYT medium. Furthermore, EcN BGs were cultivated in a fermenter, achieving an initial OD600 as high as 6.0 after optimization, indicating enhanced BG production. Moreover, the yield of ID52-E-W4A-induced BGs reached 67.0%, contrasting with only a 3.1% yield from φX174-E-induced BGs. The extended applicability of the lysis protein ID52-E-W4A was demonstrated through the preparation of Salmonella pullorum ghosts and Salmonella choleraesuis ghosts. Knocking out the molecular chaperone gene slyD and dnaJ revealed that ID52-mediated BGs could still undergo lysis. Conversely, overexpression of integral membrane enzyme gene mraY resulted in the loss of lysis activity for ID52-E, suggesting that the lysis protein ID52-E may no longer rely on SlyD or DnaJ to function, with MraY potentially being the target of ID52-E. This study introduces a novel approach utilizing ID52-E-W4A for recombinant expression, accelerating the BG formation and thereby enhancing BG yield and productivity.

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Weissella koreensis and Pediococcus pentosaceus bacterial ghosts induce inflammatory responses as immunostimulants

Cited by 2 publications

References 26 publications

Assessing the Probiotic Effects of Pediococcus pentosaceus CACC616 in Weaned Piglets

Assessing the Probiotic Effects of Pediococcus pentosaceus CACC616 in Weaned Piglets

Construction and Mechanism Exploration of Highly Efficient System for Bacterial Ghosts Preparation Based on Engineered Phage ID52 Lysis Protein E

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