Weissella cibaria WIKIM28 ameliorates atopic dermatitis-like skin lesions by inducing tolerogenic dendritic cells and regulatory T cells in BALB/c mice

Lim, Seul Ki; Kwon, Min‐Sung; Lee, Jieun; Oh, Young J.; Jang, Ja-Young; Lee, Jonghee; Park, Hae Woong; Nam, Young-Do; Seo, Myung-Ji; Roh, Seong Woon; Choi, Hak-Jong

doi:10.1038/srep40040

Cited by 77 publications

(76 citation statements)

References 24 publications

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“…Probiotic bacteria have also been investigated in murine models of atopic dermatitis (AD). Here, oral supplementation with Weissela cibaria WIKIM28 isolated from gat kimchi improved skin symptoms and reduced Th2 responses, whereas amount of regulatory T cells (Treg) and IL‐10 secretion was increased . An increase of Treg cells was also demonstrated in a similar AD model using a mixture of seven Bifidobacteria and lactic acid bacteria presented in drinking water which led to reduced Th2 cytokine levels and ameliorated experimental disease phenotype .…”

Section: Bacterial Influences On Immune Responses and Lung Epithelialmentioning

confidence: 88%

Influences of environmental bacteria and their metabolites on allergies, asthma, and host microbiota

Jatzlauk

Bartel

Heine

et al. 2017

Allergy

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The prevalence of allergic diseases and asthma has dramatically increased over the last decades, resulting in a high burden for patients and healthcare systems. Thus, there is an unmet need to develop preventative strategies for these diseases. Epidemiological studies show that reduced exposure to environmental bacteria in early life (eg, birth by cesarean section, being formula-fed, growing up in an urban environment or with less contact to various persons) is associated with an increased risk to develop allergies and asthma later in life. Conversely, a reduced risk for asthma is consistently found in children growing up on traditional farms, thereby being exposed to a wide spectrum of microbes. However, clinical studies with bacteria to prevent allergic diseases are still rare and to some extent contradicting. A detailed mechanistic understanding of how environmental microbes influence the development of the human microbiome and the immune system is important to enable the development of novel preventative approaches that are based on the early modulation of the host microbiota and immunity. In this mini-review, we summarize current knowledge and experimental evidence for the potential of bacteria and their metabolites to be used for the prevention of asthma and allergic diseases.

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Section: Bacterial Influences On Immune Responses and Lung Epithelialmentioning

confidence: 88%

Influences of environmental bacteria and their metabolites on allergies, asthma, and host microbiota

Jatzlauk

Bartel

Heine

et al. 2017

Allergy

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“…Les mécanismes permettant l'atténuation des symptômes de la DA ont été étudiés par ces mêmes équipes de recherche et l'implication de cellules dendritiques (CD) tolérogènes a été mise en évidence [5][6][7][8]. En effet, lorsque des CD de souris atteintes de DA sont traitées par des probiotiques, une augmentation des marqueurs spécifiques des CD tolérogènes est observée [5][6][7][8].…”

Section: L'induction De Cellules Dendritiques Tolérogènes Par Les Prounclassified

Traiter la dermatite atopique par les probiotiques

Lopez-Ramirez

Masse

2019

Med Sci (Paris)

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Dans le cadre du module d’enseignement Communication Scientifique et Littérature du Master Biologie Moléculaire et Cellulaire de Lyon, les étudiants des parcours M2 Génopath et Biologie de la Peau se sont formés à l’écriture scientifique sur un sujet libre. Suite à un travail préparatoire avec l’équipe pédagogique, chaque étudiant a rédigé, conseillé par un chercheur, une Nouvelle. Le parcours M2 Génopath s’adresse aux étudiants scientifiques et médecins et les forme à la recherche fondamentale dans les domaines de la génétique, de la biologie cellulaire et de leurs applications biomédicales. Le parcours M2 Biologie de la Peau est une formation unique en France, et forme des spécialistes de la recherche en biologie cutanée qui s’inséreront dans les services de recherche et développement hospitalier ou de l’industrie dermo-cosmétique et dermo-pharmaceutique.

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“…There is clinical evidence that Weissella cibaria , a lactic acid bacterium that is often included in fermented vegetable preparations, has health benefits (Amandine & Remize, ; Kang, Piao, Shin, Lee, & Oh, ; Kwak, Cho, Noh, & Om, ). The W. cibaria JW15 strain (JW15), which is found in kimchi, has anti‐inflammatory and immune modulating effects (Ahn et al., ; Lim et al., ; Nathan, Courtney, Amber Walters, & Chastona, ; Park, Kang, Kim, Lee, & Lee, ). The findings of these studies support the usefulness of JW15 as a probiotic for improving health.…”

Section: Introductionmentioning

confidence: 99%

Optimal conditions for the encapsulation of Weissella cibaria JW15 using alginate and chicory root and evaluation of capsule stability in a simulated gastrointestinal system

Kim

Nam

et al. 2020

Journal of Food Science

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The delivery of active probiotic cells in capsules can reduce probiotic cell loss induced by detrimental external factors during digestion. In this study, we determined the optimal conditions for the encapsulation of Weissella cibaria JW15 (JW15) within calcium and polyethylene glycol (PEG)-alginate with chicory root extract powder (CREP). JW15 was encapsulated as the core material (10 9 cells/mL, 2 mL/min), and a solution containing a mixture of 1.5% sodium alginate and 1% CREP was extruded into a receiving bath with 0.1 M calcium chloride (CaCl 2 ) and 0.05% PEG. Capsule morphology and size were measured using optical microscopy. The optimal air pressure and frequency vibration for capsules containing alginate only (Al) were 200 mbar and 200 Hz, respectively and 100 mbar and 350 Hz for capsules containing alginate with CREP (Ch), respectively. The voltage for both capsules types was fixed at 1.35 kV. Then, the capsules were incubated in a simulated gastrointestinal (GI) system for 6 hr at 37°C. The addition of PEG in a CaCl 2 hardening solution led to degradation of the Ch capsule (Ch-PEG) and the release of cells into the small intestine vessel in the simulated GI system. By contrast, the cells were trapped within the Al capsules. Based on these data, effective encapsulation using alginate with CREP and PEG can enable JW15 to be released at a targeted anatomical site of activity within the GI system, thereby, enhancing the efficacy of probiotic cells. These protective effects can be leveraged during the development of probiotic products.Practical Application: Weissella cibaria JW15 (10 9 cells/mL) was encapsulated in biodegradable and biocompatible capsules, prepared by mixing 1.5% alginate with 1% chicory root extract powder (CREP) in 0.1 M CaCl 2 and 0.05% PEG using an encapsulator. The optimal processing parameters were as follows: pressure, 100 mbar; vibration frequency, 350 Hz; voltage, 1.35 kV; and core flow rate, 2 mL/min. When the resulting capsules were subjected to a simulated gastrointestinal system for 6 hr, the cells were released into the small intestine, and up to 95% cell viability was preserved. These results suggest that capsules made from alginate with CREP and formulated using calcium and PEG are a promising delivery system for probiotic cells. Further reproduction without permission is prohibitedThe encapsulation efficiency (EE) of the probiotics was calculated using the following expression (Haghshenas et al., 2015): EE (%) = (Xt/Xi) ×100, where Xt is the viability of JW15 in

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Weissella cibaria WIKIM28 ameliorates atopic dermatitis-like skin lesions by inducing tolerogenic dendritic cells and regulatory T cells in BALB/c mice

Cited by 77 publications

References 24 publications

Influences of environmental bacteria and their metabolites on allergies, asthma, and host microbiota

Influences of environmental bacteria and their metabolites on allergies, asthma, and host microbiota

Traiter la dermatite atopique par les probiotiques

Optimal conditions for the encapsulation of Weissella cibaria JW15 using alginate and chicory root and evaluation of capsule stability in a simulated gastrointestinal system

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