Weighted linear-least-squares analysis of EXSY data from multiple 1D selective inversion experiments

Perrin, Charles L.; Engler, Richard E.

doi:10.1016/0022-2364(90)90143-w

Cited by 14 publications

(19 citation statements)

References 13 publications

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“…Magnetization transfer studies were carried out as described previously (O'Connell et al, 1994) by selectively inverting the intraand extracellular 9F resonances of the glucosyl fluorides sequentially (Robinson et al, 1985) and monitoring the magnetization with a 900 pulse at delays of 0.001, 0.15, 0.30, 0.45, 0.60, and 0.75, and 20 s. The analysis of the data was based on the approach described by Perrin and Engler (1990) in which the data from the intraand extracellular resonance inversion experiments are organized into a series of 2 X 2 matrices, M(tm); each matrix is then diagonalized, and the resulting elements are fit to a linear function of time, as described below:…”

Section: Methodsmentioning

confidence: 99%

Fluorine-19 NMR studies of glucosyl fluoride transport in human erythrocytes

London¹,

Gabel²

1995

Biophysical Journal

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Fluorine-1 9 magnetization transfer studies have been used to measure the transport rate of glucopyranosyl fluorides under equilibrium exchange conditions. Although rate constants and permeabilities could be determined for ,B-D-glucopyranosyl fluoride, the exchange rate for aD -glucopyranosyl fluoride was found to be too slow for determination using this method. The time-dependent decomposition of the ,B-glucopyranosyl fluoride also limits the accuracy of the numerical results for this species; however, it is clear that the permeabilities of the a and P forms differ significantly, i.e., P > P,. This observation is in contrast to recent observations for n-fluoro-n-deoxyglucose, for which P,, > PJ, for n = 2, 3, 4, or 6. The difference can be explained in terms of a simple alternating conformation model in which one of the conformations (with an external sugar-binding site) exhibits a preference for the form of the molecule, while the second conformation (with an internal sugar binding site) exhibits a preference for the a form. Fluorine/hydroxyl substitutions unmask these preferences by selectively reducing the binding to one of the conformations, depending on the specific site of fluorination.

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Section: Methodsmentioning

confidence: 99%

Fluorine-19 NMR studies of glucosyl fluoride transport in human erythrocytes

London¹,

Gabel²

1995

Biophysical Journal

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“…Another approach to recording and analyzing 1D EXSY data, based on Eq. 33, entails linear least-squares fitting (Perrin and Engler 1990). It involves repeating the 1D EXSY measurements using the pulse sequence of Fig.…”

Section: Inversion Transfermentioning

confidence: 99%

NMR magnetization-transfer analysis of rapid membrane transport in human erythrocytes

Shishmarev

Kuchel

2016

Biophys Rev

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Nuclear magnetic resonance (NMR) magnetization-transfer (MT) experiments provide a convenient tool for studying rapid sub-second membrane-transport processes in situ in metabolically active cells. These experiments are used with membrane-permeable substances when separate (resolved) NMR signals are observed from their populations inside and outside the cells. Here, we provide a description of the theory and practice of the most common NMR MT experiments that have been used to study membrane-transport processes in human erythrocytes (red blood cells; RBCs). The procedures, involved in the analysis of the experimental data for defining mechanisms of transport, and for estimating values of kinetic parameters in the corresponding mathematical models, are given special attention.

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“…Multiple 1D selective inversion experiments 39 or multiple 1D-EXSY experiments 40 are also termed '1D-EXSY.' Accurate rate constants are obtained using weighted linear-least-squares analysis of the data.…”

Section: D Sexsymentioning

confidence: 99%

Dynamic NMR: combined use of 1D selective EXSY and complete lineshape analysis of spectra subjected to reference deconvolution and linear prediction or the maximum entropy method

Vassilev

Dimitrov

2001

Magnetic Reson in Chemistry

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In dynamic NMR lineshape analysis and in handling other curve-fitting problems, the quality of the adjusted parameters improves if the iterative procedure is constrained by introducing additional conditions. It is demonstrated on a simple model of a two-site exchange system that in the complete lineshape analysis of spectra subjected to reference deconvolution and linear prediction or the maximum entropy method in combination with 1D EXSY experiments, the unknown parameters can be reduced to only one, the rate constant. This approach ensures excellent results for the activation parameters. It is also shown that 1D selective EXSY (1D SEXSY) experiments using E-BURP pulses could successfully be used as a substitute to the more (experimentally) demanding 2D EXSY measurements.

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Weighted linear-least-squares analysis of EXSY data from multiple 1D selective inversion experiments

Cited by 14 publications

References 13 publications

Fluorine-19 NMR studies of glucosyl fluoride transport in human erythrocytes

Fluorine-19 NMR studies of glucosyl fluoride transport in human erythrocytes

NMR magnetization-transfer analysis of rapid membrane transport in human erythrocytes

Dynamic NMR: combined use of 1D selective EXSY and complete lineshape analysis of spectra subjected to reference deconvolution and linear prediction or the maximum entropy method

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