Web-Based Telemicroscopy

Hadida-Hassan, Martin; Young, Stephen J.; Peltier, Steven T.; Wong, Mona; LaMont, S. P.; Ellisman, Mark H.

doi:10.1006/jsbi.1999.4095

Cited by 59 publications

(34 citation statements)

References 20 publications

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“…We have taken advantage of these capabilities to satisfy our need for 24-h access to the laser microscope and to facilitate collaboration and networking with investigators from other locations around the world. The remote operation of microscope systems has been demonstrated for electron microscopes (Chumbley et al, 2002;Hadida-Hassan et al, 1999;Takaoka et al, 2000;Yamada et al, 2003), for light microscope evaluation of fixed samples (Kaplan et al, 2002;Molnar et al, 2003), and for laser scanning confocal microscopy (Youngblom et al, 2001). Though there has been significant progress in developing realtime microscopy and radiological image sharing over the internet, this has not been extended to the domain of real-time interventional manipulation of live cells, tissues, and organelles.…”

Section: Introductionmentioning

confidence: 99%

Internet‐based robotic laser scissors and tweezers microscopy

Botvinick

Berns

2005

Microscopy Res & Technique

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We have engineered a robotic laser ablation and tweezers microscope that can be operated via the internet using most internet accessible devices, including laptops, desktop computers, and personal data assistants (PDAs). The system affords individual investigators the ability to conduct micromanipulation experiments (cell surgery or trapping) from remote locations (i.e., between the US and Australia). This system greatly expands the availability of complex and expensive research technologies via investigator-networking over the internet. It serves as a model for other ''internet-friendly'' technologies leading to large scale networking and data-sharing between investigators, groups, and institutions on a global scale. The system offers three unique features: (1) the freedom to operate the system from any internet-capable computer, (2) the ability to image, ablate, and/or trap cells and their organelles by ''remote-control,'' and (3) the security and convenience of controlling the system in the laboratory on the user's own personal computer and not on the host machine. Four ''proof of principle'' experiments were conducted: (1) precise control of microscope movement and live cell visualization, (2) subcellular microsurgery on the microtubule organizing center of live cells viewed under phase contrast and fluorescence microscopy, (3) precise targeting of multiple sites within single red blood cells, and (4) optical trapping of 10 lm diameter polystyrene microspheres. Microsc. Res. Tech. 68:65-74, 2005. V

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Section: Introductionmentioning

confidence: 99%

Internet‐based robotic laser scissors and tweezers microscopy

Botvinick

Berns

2005

Microscopy Res & Technique

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“…Recently, remote operation systems for transmission electron microscopes (TEMs) via network connections have been developed by some research groups in the field of structural biology [6,7]. For these purposes, remote links must provide high-rate transmission with short latencies in order to make the instrument available as a research tool [10].…”

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confidence: 99%

Progressive Image Transmission in Telemicroscopy: A Quantitative Approach for Electron Microscopy Images of Biological Specimens

Ruiz¹

2002

Real-Time Imaging

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T his work presents a rigorous and objective evaluation of two progressive image transmission techniques in a framework of telemicroscopy of biological specimens. First of all, a Lossless Progressive Image Codec (LPIC) which is based on a specific wavelet transform and an efficient encoding method is introduced. This system is then compared to the standard Progressive-JPEG (P-JPEG) for the specific task of detecting biological specimens in the images progressively received by a biologist controlling a remote transmission electron microscope (TEM). Both methods have been quantitatively compared by means of a taskoriented methodology that guarantees an objective comparison for the task at hand. Such a methodology is based upon a wide ensemble of artificial images resembling real electron microscopy images, as well as on a thorough set of figures of merit assessing the quality of the reconstructed images. The results that have been obtained allow to claim with statistical significance that our method outperforms the standard P-JPEG throughout the transmission process. Finally, both the progressive transmission methods LPIC and P-JPEG were applied over real electron microscopy images from different subfields of structural biology, and the results exhibited a similar behavior to that obtained for artificial images. Visualization results manifest that LPIC exhibits excellent levels of details from the very beginning, whereas P-JPEG undergoes severe blocking artifacts along the first stages of the transmission. Therefore, the conclusion of the work is that the superiority of the progressive image transmission method introduced here over P-JPEG becomes manifest for the task of detecting biological specimens in the progressively transmitted images.

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“…Its web-based tool [3] and EMWorkspace [4] are innovative and similar to our system. However, they have some major drawbacks.…”

Section: Other Researchmentioning

confidence: 99%

HVEM Control System Based on Grid: A Cornerstone of e-Biology

Han

Kim

Jung

et al. 2007

Computational Science – ICCS 2007

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Abstract. This paper proposes HVEM Control System, which is the cornerstone for teleinstrumentation infrastructure. The proposed system is oriented for people whose primary work is to get access to a remotely located instrument, a High-Voltage Electron Microscope (HVEM). Our system is implemented to fulfill all the necessary requirements in allowing the user to 1) control every single part of HVEM in a fine-grained manner, 2) check the HVEM and observe various states of sample, and 3) take their high resolution images of the sample. In that aspect, this paper suggests an HVEM Control System designed on the concept of the Grid and Web Service which satisfies various types of user groups, explains novel characteristics of our system.

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Web-Based Telemicroscopy

Cited by 59 publications

References 20 publications

Internet‐based robotic laser scissors and tweezers microscopy

Internet‐based robotic laser scissors and tweezers microscopy

Progressive Image Transmission in Telemicroscopy: A Quantitative Approach for Electron Microscopy Images of Biological Specimens

HVEM Control System Based on Grid: A Cornerstone of e-Biology

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