Weakly acidic carboxy group-grafted β-cyclodextrin-threaded acid-degradable polyrotaxanes for modulating protein interaction and cellular internalization

Abstract

Yui (2021): Weakly acidic carboxy group-grafted β-cyclodextrin-threaded acid-degradable polyrotaxanes for modulating protein interaction and cellular internalization, Science and Technology of Advanced Materials,

Terminal Structure of Triethylene Glycol-Tethered Chains on β-Cyclodextrin-Threaded Polyrotaxanes Dominates Temperature Responsivity and Biointeractions

“…An acid-degradable PRX composed of β-CDs threaded with a poloxamer (PEG- b -poly­(propylene glycol) (PPG)- b -PEG, Pluronic P123; M n,Pluronic P123 = 5570, the number of monomer repeating units in the PEG and PPG segments are 19.5 × 2 ( M n,PEG = 860 × 2) and 66.3 ( M n,PPG = 3850), respectively) chain, wherein the terminals of the axial polymer were capped with acid-labile N -(triphenylmethyl)­glycine, was synthesized and characterized based on our previous study . The number of threading β-CDs in the PRX and the number-average molecular weight ( M n ) of the PRX were 12.3 and 20,200, respectively.…”

“…The quartz crystal microbalance with dissipation (QCM-D) monitoring was performed at 25 °C using a QSence E1 (Biolin Scientific AB, Gothenburg, Sweden). Lipid bilayer-coated sensor chip surfaces were prepared on a QSX 303 SiO 2 sensor (Biolin Scientific AB) using 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC; Avanti Polar Lipids, Alabaster, USA) as described previously . For the lipid-coated sensor chips, tris­(hydroxymethyl)­aminomethane (Tris) buffer (10 mM Tris-HCl, 150 mM NaCl, pH 7.4) solution containing TEG–PRXs (1 mM threaded β-CD) was injected for 5 min at a flow rate of 100 μL/min at 25 °C using a peristaltic pump (Reglo Digital M2-2/12, Ismatec, Wertheim, Germany).…”

“…Lipid bilayer-coated sensor chip surfaces were prepared on a QSX 303 SiO 2 sensor (Biolin Scientific AB) using 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC; Avanti Polar Lipids, Alabaster, USA) as described previously. 32 For the lipidcoated sensor chips, tris(hydroxymethyl)aminomethane (Tris) buffer (10 mM Tris-HCl, 150 mM NaCl, pH 7.4) solution containing TEG−PRXs (1 mM threaded β-CD) was injected for 5 min at a flow rate of 100 μL/min at 25 °C using a peristaltic pump (Reglo Digital M2-2/12, Ismatec, Wertheim, Germany). The buffer flow was subsequently stopped, and the lipid-coated sensor chips were incubated for 10 min to allow the TEG−PRXs to be adsorbed onto the lipid surfaces.…”

“…Protein-immobilized sensor chip surfaces were prepared on QSX 301 gold sensor chips (Biolin Scientific AB) using bovine serum albumin (BSA; Fujifilm Wako Pure Chemical) as described previously. 32 The TEG−PRX solutions (1 mM threaded β-CD in PBS) were injected for 30 min at a flow rate of 50 μL/min at 25 °C, followed by a 60 min incubation, to allow adsorption of the TEG− PRXs onto the BSA-immobilized surfaces. Finally, the sensor chip surfaces were rinsed with PBS for 20 min at a flow rate of 50 μL/min, and the ΔF and ΔD values of the third overtone were recorded.…”

Regulation of stem cell fate and function by using bioactive materials with nanoarchitectonics for regenerative medicine

“…In order to develop functional materials, it is important to prepare the materials through fundamental organic chemistry [ 1–3 ], inorganic chemistry [ 4–6 ], polymer chemistry [ 7–9 ], supramolecular chemistry [ 10–12 ], material science [ 13–16 ], and biologically related science [ 17–19 ]. Besides the materials themselves, the internal structure of the materials and the organized mode of the components have a significant impact on the efficiency and selectivity of their functions.…”