Weak acids as an alternative anti-microbial therapy

Kundukad, Binu; Udayakumar, Gayathri; Grela, Erin; Kaur, Dhamanpreet; Rice, Scott A.; Kjelleberg, Staffan; Doyle, Patrick S.

doi:10.1016/j.bioflm.2020.100019

Cited by 48 publications

(35 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, lactic acid was a major compound responsible for the inhibitory effects observed. The efficacy of weak acids to control the growth of microbial pathogens was reported previously (Shokri 2011 ; Sharma and Srivastava 2014 ) and it was more significant when the pH was < pKa value (Kundukad et al 2020 ). Although the pH of both lactic and acetic acid in our study was adjusted to pH 3.33 (< pKa), no activity was observed for acetic acid.…”

Section: Discussionsupporting

confidence: 53%

Lactobacillus plantarum strain HT-W104-B1: potential bacterium isolated from Malaysian fermented foods for control of the dermatophyte Trichophyton rubrum

Danial

Medina

Magan

2021

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The objective was to screen and evaluate the anti-fungal activity of lactic acid bacteria (LABs) isolated from Malaysian fermented foods against two Trichophyton species. A total of 66 LAB strains were screened using dual culture assays. This showed that four LAB strains were very effective in inhibiting growth of T. rubrum but not T. interdigitale. More detailed studies with Lactobacillus plantarum strain HT-W104-B1 showed that the supernatant was mainly responsible for inhibiting the growth of T. rubrum. The minimum inhibitory concentration (MIC), inhibitory concentration, the 50% growth inhibition (IC50) and minimum fungicide concentration (MFC) were 20 mg/mL, 14 mg/mL and 30 mg/mL, respectively. A total of six metabolites were found in the supernatant, with the two major metabolites being L-lactic acid (19.1 mg/g cell dry weight (CDW)) and acetic acid (2.2 mg/g CDW). A comparative study on keratin agar media showed that the natural mixture in the supernatants predominantly contained L-lactic and acetic acid, and this significantly controlled the growth of T. rubrum. The pure two individual compounds were less effective. Potential exists for application of the natural mixture of compounds for the treatment of skin infection by T. rubrum.

show abstract

Section: Discussionsupporting

confidence: 53%

Lactobacillus plantarum strain HT-W104-B1: potential bacterium isolated from Malaysian fermented foods for control of the dermatophyte Trichophyton rubrum

Danial

Medina

Magan

2021

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…The droplets are left to incubate at room temperature for 24 h, during which time the bacteria consolidate at the center of the droplet. We then inject known volumes of citric acid solution, a weak acid drug (49,50), and a dead stain into each of the arrayed droplets ( Fig. 3B) and obtain a range of final drug concentrations.…”

Section: Relative Frequencymentioning

confidence: 99%

Embedded droplet printing in yield-stress fluids

Nelson

Kundukad

Wong

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Microfluidic tools and techniques for manipulating fluid droplets have become core to many scientific and technological fields. Despite the plethora of existing approaches to fluidic manipulation, non-Newtonian fluid phenomena are rarely taken advantage of. Here we introduce embedded droplet printing—a system and methods for the generation, trapping, and processing of fluid droplets within yield-stress fluids, materials that exhibit extreme shear thinning. This technique allows for the manipulation of droplets under conditions that are simply unattainable with conventional microfluidic methods, namely the elimination of exterior influences including convection and solid boundaries. Because of this, we believe embedded droplet printing approaches an ideal for the experimentation, processing, or observation of many samples in an “absolutely quiescent” state, while also removing some troublesome aspects of microfluidics including the use of surfactants and the complexity of device manufacturing. We characterize a model material system to understand the process of droplet generation inside yield-stress fluids and develop a nascent set of archetypal operations that can be performed with embedded droplet printing. With these principles and tools, we demonstrate the benefits and versatility of our method, applying it toward the diverse applications of pharmaceutical crystallization, microbatch chemical reactions, and biological assays.

show abstract

“…1). So far, the highest antimicrobial activity of CA has been demonstrated at low pH, mainly between the first and second pK a values (Young and Foegeding 1993;Buchanan and Golden 1994;Kundukad et al 2020). Knowledge of the mechanism of CA's antimicrobial action remains limited but it is believed that at low pH, CA in its un-charged, un-dissociated state (CAH 3 ) is able to freely cross the microbial membrane.…”

Section: Introductionmentioning

confidence: 99%

Impact of pH on citric acid antimicrobial activity against Gram‐negative bacteria

Burel

Kala

Purevdorj-Gage

2020

Lett Appl Microbiol

View full text Add to dashboard Cite

Significance and Impact of the Study: Citric acid (CA) is an antimicrobial molecule with three ionization states that exist in an equilibrium directly governed by pH. Traditionally, non-ionized CA was considered more antimicrobial, presumably due to the combined effects of the molecule and the acidic environment in which it occurs. By decoupling the antimicrobial impact of pH and CA on Gram-negative bacteria, it is demonstrated, for the first time, that the fully ionized CA species alone was the most effective at destroying the bacteria. The results from SEM imaging and surface-charge measurements provide further insight into the antimicrobial mode of action of CA against bacteria.

show abstract

Weak acids as an alternative anti-microbial therapy

Cited by 48 publications

References 54 publications

Lactobacillus plantarum strain HT-W104-B1: potential bacterium isolated from Malaysian fermented foods for control of the dermatophyte Trichophyton rubrum

Lactobacillus plantarum strain HT-W104-B1: potential bacterium isolated from Malaysian fermented foods for control of the dermatophyte Trichophyton rubrum

Embedded droplet printing in yield-stress fluids

Impact of pH on citric acid antimicrobial activity against Gram‐negative bacteria

Contact Info

Product

Resources

About